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Literature summary for 1.1.1.324 extracted from

  • Singh, S.K.; Patra, B.; Paul, P.; Liu, Y.; Pattanaik, S.; Yuan, L.
    BHLH iridoid synthesis 3 is a member of a bHLH gene cluster regulating terpenoid indole alkaloid biosynthesis in Catharanthus roseus (2021), Plant Direct, 5, e00305 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene 8HGO, reporter plasmids for transient protoplast assays of enzyme expression are generated by cloning the GES, G10H, 8HGO, IS, IO, 7-DLGT, and 7DLH promoters upstream of a firefly luciferase (LUC) reporter and rbcS terminator Catharanthus roseus

Protein Variants

Protein Variants Comment Organism
additional information BIS3 overexpression in Catharanthus roseus flower petal upregulates terpenoid indole alkaloid (TIA) pathway gene expression Catharanthus roseus

Localization

Localization Comment Organism GeneOntology No. Textmining
protoplast
-
 Catharanthus roseus
-
-

Organism

Organism UniProt Comment Textmining
Catharanthus roseus W8JWV8
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower low expression level Catharanthus roseus
-
leaf low expression level Catharanthus roseus
-
root high expression level Catharanthus roseus
-
stem low expression level Catharanthus roseus
-

Synonyms

Synonyms Comment Organism
8-hydroxygeraniol oxidoreductase
-
 Catharanthus roseus
8HGO
-
 Catharanthus roseus
ADH10 UniProt Catharanthus roseus
alcohol dehydrogenase 10 UniProt Catharanthus roseus

Expression

Organism Comment Expression
Catharanthus roseus the enzyme expression is activated by transcription factor BIS3, i.e. BHLH iridoid synthesis 3, a basic helix-loop-helix (bHLH) transcription factor and key regulators of plant specialized metabolites, including terpenoid indole alkaloids, in Catharanthus roseus plants. BIS3 is clustered with BIS2 and BIS1 in a bHLH gene cluster. Similar to the iridoid pathway genes, BIS3 is highly expressed in roots and induced by methyl jasmonate. Transactivation of the promoters is abolished when BIS3 is converted to a dominant repressor by fusing with the ERF-associated amphiphilic repression (EAR) sequence (BIS3-SRDX) up

General Information

General Information Comment Organism
metabolism the enzyme is involved in the iridoid pathway Catharanthus roseus