Literature summary for 1.1.1.324 extracted from

Hallahan, D.; West, J.; Wallsgrove, R.; Smiley, D.; Dawson, G.; Pickett, J.; Hamilton, J.
Purification and characterization of an acyclic monoterpene primary alcohol:NADP+ oxidoreductase from catmint (Nepeta racemosa) (1995), Arch. Biochem. Biophys., 318, 105-112.

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Application

Application	Comment	Organism
additional information	plant (4aR,7S,7aS)-nepetalactone can be used for synthesis of (4aR,7S,7aS)-nepetalactol, an aphid pheromone, useful in aphid control	Nepeta racemosa

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	79% inhibition at 1.0 mM, 87% at 10 mM	Nepeta racemosa
4-chloromercuribenzoate	complete inhibition at 1 mM and above	Nepeta racemosa
EDTA	44% inhibition at 100 mM	Nepeta racemosa
iodoacetamide	5% inhibition at 1.0 mM, 22% at 10 mM	Nepeta racemosa
NEM	55% inhibition at 1.0 mM, complete inhibition at 10 mM	Nepeta racemosa
Triton X-100	potent inhibition at 0.01-0.1%	Nepeta racemosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.018	-	NADP+	with 8-hydroxynerol, pH 9.5, 25°C	Nepeta racemosa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Nepeta racemosa	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	the enzyme contains zinc ions	Nepeta racemosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	1 * 40000 + 1 x 42000, SDS-PAGE	Nepeta racemosa
63000	-	gel filtration	Nepeta racemosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(6E)-8-hydroxygeraniol + 2 NADP+	Nepeta racemosa	-	(6E)-8-oxogeranial + 2 NADPH + 2 H+	-	?
(6E)-8-hydroxynerol + 2 NADP+	Nepeta racemosa	-	? + 2 NADPH + 2 H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Nepeta racemosa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme 1149fold from leaves to homogeneity by ultracentrifugation, ammonium sulfate fractionation, hydrophobic inetraction and anion exchange chromatography, affinity chromatography, and gel filtration	Nepeta racemosa

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Nepeta racemosa	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1417	-	purified enzyme, pH 9.5, 25°C	Nepeta racemosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(6E)-8-hydroxygeraniol + 2 NADP+	-	Nepeta racemosa	(6E)-8-oxogeranial + 2 NADPH + 2 H+	-	?
(6E)-8-hydroxygeraniol + 2 NADP+	oxidization at C1 and C8, depending on numbering of the molecule, GC-MS product analysis	Nepeta racemosa	(6E)-8-oxogeranial + 2 NADPH + 2 H+	-	?
(6E)-8-hydroxynerol + 2 NADP+	-	Nepeta racemosa	? + 2 NADPH + 2 H+	-	?
(6E)-8-hydroxynerol + 2 NADP+	oxidization at C1 and C8, depending on numbering of the molecule, GC-MS product analysis	Nepeta racemosa	? + 2 NADPH + 2 H+	-	?

Subunits

Subunits	Comment	Organism
dimer	1 * 40000 + 1 x 42000, SDS-PAGE	Nepeta racemosa

Synonyms

Synonyms	Comment	Organism
acyclic monoterpene primary alcohol:NADP+ oxidoreductase	-	Nepeta racemosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Nepeta racemosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9.5	-	-	Nepeta racemosa

Cofactor

Cofactor	Comment	Organism	Structure
additional information	no activity with NAD+	Nepeta racemosa
NADP+	dependent on	Nepeta racemosa

General Information

General Information	Comment	Organism
metabolism	the enzyme plays a role in the biosynthesis of iridoid monoterpenes, e.g. (4aR,7S,7aS)-nepetalactone	Nepeta racemosa

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Release 2023.1 (January 2023)