KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.64 | - |
benzil | pH 8.0, 30°C | Ogataea glucozyma |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea glucozyma | A0A0H4SN47 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(2-fluorophenyl)ethanone + NADPH + H+ | 76% of the rate with 1-(4-nitrophenyl)ethanone, 97% yield, 94% enantiomeric excess | Ogataea glucozyma | (1S)-1-(2-fluorophenyl)ethanol + NADP+ | - |
? | |
1-(2-nitrophenyl)ethanone + NADPH + H+ | 53% of the rate with 1-(4-nitrophenyl)ethanone, 78% yield, 98% enantiomeric excess | Ogataea glucozyma | (1S)-1-(2-nitrophenyl)ethanol + NADP+ | - |
? | |
1-(3-fluorophenyl)ethanone + NADPH + H+ | 61% of the rate with 1-(4-nitrophenyl)ethanone, 85% yield, 98% enantiomeric excess | Ogataea glucozyma | (1S)-1-(3-fluorophenyl)ethanol + NADP+ | - |
? | |
1-(4-nitrophenyl)ethanone + NADPH + H+ | 100% yield, 98% enantiomeric excess | Ogataea glucozyma | (1S)-1-(4-nitrophenyl)ethanol + NADP+ | - |
? | |
1-(furan-2-yl)ethanone + NADPH + H+ | - |
Ogataea glucozyma | (1S)-1-(furan-2-yl)ethanol + NADP+ | 48% of the activity with benzil, 95% yield, 98% enantiomeric excess | ? | |
1-(pyridin-3-yl)ethanone + NADPH + H+ | - |
Ogataea glucozyma | (S)-(1-pyridin-3yl)ethanol + NADP+ | 58% of the activity with benzil, 95% yield, 98% enantiomeric excess | ? | |
1-(pyridin-3-yl)propan-1-one + NADPH + H+ | - |
Ogataea glucozyma | (S)-1-(pyridin-3-yl)propanol + NADP+ | 60% of the activity with benzil, 95% yield, 98% enantiomeric excess | ? | |
1-(thiophen-2-yl)ethanone + NADPH + H+ | - |
Ogataea glucozyma | (1S)-1-(thiophen-2-yl)ethanol + NADP+ | 51% of the activity with benzil, 95% yield, 98% enantiomeric excess | ? | |
2-acetylbenzonitrile + NADPH + H+ | 82% of the rate with 1-(4-nitrophenyl)ethanone, 98% yield, 98% enantiomeric excess | Ogataea glucozyma | 2-[(1S)-1-hydroxyethyl]benzonitrile + NADP+ | - |
? | |
3-oxo-3-phenylpropanenitrile + NADPH + H+ | - |
Ogataea glucozyma | (3S)-3-hydroxy-3-phenylpropanenitrile + NADP+ | 55% of the activity with benzil, 95% yield, 95% enantiomeric excess | ? | |
4-acetylbenzonitrile + NADPH + H+ | 68% of the rate with 1-(4-nitrophenyl)ethanone, 70% yield, 97% enantiomeric excess | Ogataea glucozyma | 4-[(1S)-1-hydroxyethyl]benzonitrile + NADP+ | - |
? | |
benzil + NADPH + H+ | - |
Ogataea glucozyma | (S)-benzoin + NADP+ | 98% enantiomeric excess | ? | |
benzophenone + NADPH + H+ | - |
Ogataea glucozyma | diphenylmethanol + NADP+ | 66% yield | ? | |
ethyl 2-oxo-2-phenylacetate + NADPH + H+ | - |
Ogataea glucozyma | ethyl (2S)-hydroxy(phenyl)acetate + NADP+ | 115% yield, 40% enantiomeric excess | ? | |
additional information | for the enantioselective reduction of mono-substituted acetophenones, reaction rates of meta- and para-derivatives are consistent with the electronic effects described by delta-Hammett coefficients. Enantioselectivity is determined by an opposite orientation of the substrate in the binding pocket. Reduction of ortho-derivatives occurs only with substrates bearing substituents with low steric impact, and reactivity is controlled by stereoelectronic features | Ogataea glucozyma | ? | - |
? | |
additional information | the enzyme prefers space-demanding substrates, which are often converted with high stereoselectivity | Ogataea glucozyma | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KRED1-Pglu | - |
Ogataea glucozyma |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | - |
Ogataea glucozyma |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable up to | Ogataea glucozyma |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
benzil | pH 8.0, 30°C | Ogataea glucozyma |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Ogataea glucozyma |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
90% of maximum activity | Ogataea glucozyma |
8 | - |
90% of maximum activity | Ogataea glucozyma |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
benzil | pH 8.0, 30°C | Ogataea glucozyma |