Cloned (Comment) | Organism |
---|---|
gene yueD, Bacillus cereus library screening in Escherichia coli and cloning of the gene encoding the benzil reductase, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strain DH5alpha | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-benzoin + NADP+ | Bacillus cereus | stereospecific asymmetric reduction of benzil to (S)-benzoin | benzil + NADPH + H+ | - |
r | |
(S)-benzoin + NADP+ | Bacillus cereus Tim-r01 | stereospecific asymmetric reduction of benzil to (S)-benzoin | benzil + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | Q8RJB2 | - |
- |
Bacillus cereus Tim-r01 | Q8RJB2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-benzoin + NADP+ | stereospecific asymmetric reduction of benzil to (S)-benzoin | Bacillus cereus | benzil + NADPH + H+ | - |
r | |
(S)-benzoin + NADP+ | stereospecific asymmetric reduction of benzil to (S)-benzoin | Bacillus cereus Tim-r01 | benzil + NADPH + H+ | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Bacillus cereus | |
NADPH | - |
Bacillus cereus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the short-chain dehydrogenase/reductase, SDR, family | Bacillus cereus |