Cloned (Comment) | Organism |
---|---|
gene EGS1, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter. Analysis of ObEGS1 and ObEGS4 endogenous promoters shows the presence of different cis regulatory elements, expression pattern. An AC-II element is found in ObEGS1 promoter and a MYB biding site (MBS) is found in ObEGS4 | Ocimum basilicum |
gene EGS2, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter | Ocimum basilicum |
gene EGS3, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter | Ocimum basilicum |
gene EGS4, genotyping and quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter. Analysis of ObEGS1 and ObEGS4 endogenous promoters shows the presence of different cis regulatory elements, expression pattern. An AC-II element is found in ObEGS1 promoter and a MYB biding site (MBS) is found in ObEGS4 | Ocimum basilicum |
gene EGS5, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter | Ocimum basilicum |
gene EGS6, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter | Ocimum basilicum |
gene EGS7, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter | Ocimum basilicum |
gene EGS8, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter | Ocimum basilicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Ocimum basilicum | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coniferyl acetate + NADPH + H+ | Ocimum basilicum | - |
eugenol + acetate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ocimum basilicum | - |
- |
- |
Ocimum basilicum | A0A1B2U6R8 | - |
- |
Ocimum basilicum | A0A1B2U6S4 | - |
- |
Ocimum basilicum | A0A1B2U6T3 | - |
- |
Ocimum basilicum | A0A7S9C1H0 | - |
- |
Ocimum basilicum | A0A7S9C1M1 | - |
- |
Ocimum basilicum | A0A7U3PJH6 | - |
- |
Ocimum basilicum | Q15GI4 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | posttranscriptional modifications can regulate eugenol synthase in the roots of sweet basil | Ocimum basilicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Ocimum basilicum | - |
leaf | leaves and leaves stripped of PGTs | Ocimum basilicum | - |
additional information | isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS1 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves, not in roots and leaves stripped of PGTs | Ocimum basilicum | - |
additional information | isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS2 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves and roots, but not in leaves stripped of PGTs | Ocimum basilicum | - |
additional information | isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS3 is expressed in peltate glandular trichomes (PGTs) and roots, but not in leaves and leaves stripped of PGTs | Ocimum basilicum | - |
additional information | isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS4 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots | Ocimum basilicum | - |
additional information | isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS5 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots, and very low expression levels in all leaves | Ocimum basilicum | - |
additional information | isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS6 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs) and leaves, but no expression in leaves stripped of PGTs | Ocimum basilicum | - |
additional information | isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS7 is expressed in all tissues analyzed with highest expression levels in leaves and leaves stripped of peltate glandular trichomes | Ocimum basilicum | - |
additional information | isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS8 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs), but no expression in leaves and leaves stripped of PGTs | Ocimum basilicum | - |
peltate glandular trichome | PGT | Ocimum basilicum | - |
root | the amount of eugenol produced in roots of aseptically-grown plants under elicitation is much lower than the amount produced in roots of soil-grown plants. This might be because of the different external factors and multiple stress conditions experienced in soil as compared to aseptic conditions | Ocimum basilicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaryl acetate + NADPH + H+ | - |
Ocimum basilicum | chavicol + acetate + NADP+ | - |
r | |
coniferyl acetate + NADPH + H+ | - |
Ocimum basilicum | eugenol + acetate + NADP+ | - |
r | |
additional information | GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes | Ocimum basilicum | ? | - |
- |
|
additional information | no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes | Ocimum basilicum | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
eugenol synthase 1 | - |
Ocimum basilicum |
eugenol synthase 2 | - |
Ocimum basilicum |
eugenol synthase 3 | - |
Ocimum basilicum |
eugenol synthase 4 | - |
Ocimum basilicum |
eugenol synthase 5 | - |
Ocimum basilicum |
eugenol synthase 6 | - |
Ocimum basilicum |
eugenol synthase 7 | - |
Ocimum basilicum |
eugenol synthase 8 | - |
Ocimum basilicum |
ObEGS1 | - |
Ocimum basilicum |
ObEGS2 | - |
Ocimum basilicum |
ObEGS3 | - |
Ocimum basilicum |
ObEGS4 | - |
Ocimum basilicum |
ObEGS5 | - |
Ocimum basilicum |
ObEGS6 | - |
Ocimum basilicum |
ObEGS7 | - |
Ocimum basilicum |
ObEGS8 | - |
Ocimum basilicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Ocimum basilicum | |
NADPH | - |
Ocimum basilicum |
Organism | Comment | Expression |
---|---|---|
Ocimum basilicum | methyl jasmonate, pectinase, and infection by a fungus can induce eugenol production in aseptically grown roots | up |
Ocimum basilicum | methyl jasmonate, pectinase, and infection by fungus can induce eugenol production in aseptically grown roots | up |
General Information | Comment | Organism |
---|---|---|
evolution | Ocimum basilicum contains 8 isozymes of eugenol synthase, EGS. The enzymes belong to the PIP family, named after the first three identified members, pinoresinollariciresinol reductase (PLR), isoflavone reductase (IFR), and phenylcoumaran benzylic ether reductase (PCBER) | Ocimum basilicum |