BRENDA - Enzyme Database
show all sequences of 1.1.1.317

Expression, purification, crystallization and preliminary X-ray analysis of perakine reductase, a new member of the aldo-keto reductase enzyme superfamily from higher plants

Rosenthal, C.; Mueller, U.; Panjikar, S.; Sun, L.; Ruppert, M.; Zhao, Y.; Stöckigt, J.; Acta Crystallogr. Sect. F 62, 1286-1289 (2006)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
heterologous expression of a triple mutant (K98A/K242A/K294A) of perakine reductase in Escherichia coli
Rauvolfia serpentina
Crystallization (Commentary)
Crystallization (Commentary)
Organism
crystals of the purified and methylated enzyme are obtained by the hanging-drop vapour diffusion technique at 20°C with 100 mM sodium citrate pH 5.6 and 27% PEG 4000 as precipitant. Crystals belong to space group C2221 and diffract to 2.0 A, with unit-cell parameters a = 58.9, b = 93.0, c = 143.4 A
Rauvolfia serpentina
Organism
Organism
UniProt
Commentary
Textmining
Rauvolfia serpentina
Q3L181
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Rauvolfia serpentina
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
probable dual functionality of perakine reductase, which may not only include alkaloid biosynthesis but may also include function in phenylpropanoid metabolism, generating cinnamic alcohols
713623
Rauvolfia serpentina
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
heterologous expression of a triple mutant (K98A/K242A/K294A) of perakine reductase in Escherichia coli
Rauvolfia serpentina
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystals of the purified and methylated enzyme are obtained by the hanging-drop vapour diffusion technique at 20°C with 100 mM sodium citrate pH 5.6 and 27% PEG 4000 as precipitant. Crystals belong to space group C2221 and diffract to 2.0 A, with unit-cell parameters a = 58.9, b = 93.0, c = 143.4 A
Rauvolfia serpentina
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rauvolfia serpentina
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
probable dual functionality of perakine reductase, which may not only include alkaloid biosynthesis but may also include function in phenylpropanoid metabolism, generating cinnamic alcohols
713623
Rauvolfia serpentina
?
-
-
-
-
Other publictions for EC 1.1.1.317
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725466
Sun
Crystal structure of perakine ...
Rauvolfia serpentina
J. Biol. Chem.
287
11213-11221
2012
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1
1
2
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1
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2
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1
1
1
1
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1
1
1
2
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1
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1
1
1
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1
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3
3
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716558
Sun
Purification, cloning, functio ...
Rauvolfia serpentina
Plant Mol. Biol.
67
455-467
2008
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1
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5
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2
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2
1
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5
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1
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4
1
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2
2
1
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1
1
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5
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2
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1
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1
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4
1
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2
2
1
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1
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2
2
713623
Rosenthal
-
Expression, purification, crys ...
Rauvolfia serpentina
Acta Crystallogr. Sect. F
62
1286-1289
2006
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1
1
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1
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