Literature summary for 1.1.1.316 extracted from

Jiang, Z.Y.; Zhong, Y.; Zheng, J.; Ali, M.; Liu, G.D.; Zheng, X.L.
L-ascorbic acid metabolism in an ascorbate-rich kiwifruit (Actinidia eriantha Benth.) cv. White during postharvest (2018), Plant Physiol. Biochem., 124, 20-28 .

Search for more literature for 1.1.1.316

Cloned(Commentary)

Cloned (Comment)	Organism
quantitative real-time PCR enzyme expression analysis in Actinidia eriantha cv. White	Actinidia eriantha

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Actinidia eriantha	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-galactose + NAD+	Actinidia eriantha	-	L-galactono-1,4-lactone + NADH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Actinidia eriantha	C0LVA5	cv. White, Actinidia fulvicoma var. lanata	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit	-	Actinidia eriantha	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-galactose + NAD+	-	Actinidia eriantha	L-galactono-1,4-lactone + NADH + H+	-	r

Synonyms

Synonyms	Comment	Organism
GALDH	-	Actinidia eriantha
L-galactose dehydrogenase	-	Actinidia eriantha

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Actinidia eriantha

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	assay at	Actinidia eriantha

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Actinidia eriantha
NADH	-	Actinidia eriantha

General Information

General Information	Comment	Organism
metabolism	L-ascorbic acid (AsA) biosynthesis through the L-galactose pathway supplemented by D-galacturonic acid pathway and AsA recycling collectively contributes to accumulating and remaining higher AsA level in kiwifruit cv. White during postharvest. L-Galactose dehydrogenase (GalDH) activity and relative expressions of the genes encoding GDP-D-mannose diphosphorylase (GMP), L-galactose-1-P phosphatase (GPP), GDP-L-galactose phosphorylase (GGP), GalDH and GalUR are important for regulation of AsA biosynthesis. The genes including GMP, GME, GGP, GPP, GalDH, and GalLDH involve in L-galactose pathway. The activity and expression of dehydroascorbate reductase (DHAR) are primarily responsible for regulation of AsA recycling in kiwifruit cv. White during postharvest. Changes in activities of enzymes involved in AsA metabolism in the fruit during storage, quantitative real-time PCR expression analysis. GalDH activity is positively correlated with AsA content. GalLDH activity maintains a relatively steady level for the first 17 days of fruit storage, and then decreases a lowest level at 21 days of storage. The expression of GalDH increases sharply during the first 9 days, and then gradually increases to about 4times higher level compared to the initial level whereas, GalLDH is stably expressed with a slight fluctuation during storage	Actinidia eriantha

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Release 2023.1 (January 2023)