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Literature summary for 1.1.1.316 extracted from

  • Wheeler, G.L.; Jones, M.A.; Smirnoff, N.
    The biosynthetic pathway of vitamin C in higher plants (1998), Nature, 393, 365-369.
    View publication on PubMed
Search for more literature for 1.1.1.316

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 L-galactose pH 7.5, temperature not specified in the publication Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-galactose + NAD+ Pisum sativum a step in the Smirnoff-Wheeler pathway, ascorbate biosynthesis in higher plants L-galactono-1,4-lactone + NADH + H+
-
 ?
L-galactose + NAD+ Arabidopsis thaliana a step in the Smirnoff-Wheeler pathway, ascorbate biosynthesis in higher plants L-galactono-1,4-lactone + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
embryonic axis
-
 Pisum sativum
-
leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-galactose + NAD+
-
 Pisum sativum L-galactono-1,4-lactone + NADH + H+
-
 ?
L-galactose + NAD+
-
 Arabidopsis thaliana L-galactono-1,4-lactone + NADH + H+
-
 ?
L-galactose + NAD+ a step in the Smirnoff-Wheeler pathway, ascorbate biosynthesis in higher plants Pisum sativum L-galactono-1,4-lactone + NADH + H+
-
 ?
L-galactose + NAD+ a step in the Smirnoff-Wheeler pathway, ascorbate biosynthesis in higher plants Arabidopsis thaliana L-galactono-1,4-lactone + NADH + H+
-
 ?
additional information no activity with D-galactose, D-glucose, D-mannose, L-fucose and D-arabinose. The enzyme oxidizes L-sorbosone with very low affinity Pisum sativum ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+ the reduction activity with NADP+ is 10% of that with NAD+ Pisum sativum
NADP+ the reduction activity with NADP+ is 10% of that with NAD+ Pisum sativum