Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
His-tagged version expressed in Escherichia coli BL21(DE3)lysS, expressed in Nicotiana tabacum SRI | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-galactono-1,4-lactone | uncompetitive | Pisum sativum | |
N-ethylmaleimide | 1 mM, 90% inhibition; 90% inhibition with 1 mM | Pisum sativum | |
oxidized glutathione | 5 mM, 65% inhibition; 65% inhibition with 5 mM | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0203 | - |
NAD+ | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
0.085 | - |
L-galactose | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
0.43 | - |
L-galactose | pH 7.5, 22°C | Pisum sativum | |
0.43 | - |
L-galactose | pH 7.5, temperature not specified in the publication | Pisum sativum | |
0.59 | - |
NADP+ | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
3.7 | - |
L-gulose | pH 7.5, 22°C | Pisum sativum | |
3.7 | - |
L-gulose | pH 7.5, temperature not specified in the publication | Pisum sativum | |
56.3 | - |
L-fucose | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, SDS-PAGE | Pisum sativum |
40000 | - |
4 * 40000, gel filtration and SDS-PAGE, N-terminal sequencing | Pisum sativum |
42200 | - |
2 * 42200, SDS-PAGE and gel filtration | Arabidopsis thaliana |
42200 | - |
2 * 42200, SDS-PAGE | Arabidopsis thaliana |
87500 | - |
gel filtration | Arabidopsis thaliana |
87500 | - |
gel filtration, two peaks of activity at 42400 Da and 87500 Da | Arabidopsis thaliana |
156000 | - |
gel filtration | Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactose + NAD+ | Pisum sativum | a step in the Smirnoff-Wheeler pathway, ascorbate biosynthesis in higher plants | L-galactono-1,4-lactone + NADH + H+ | - |
ir | |
L-galactose + NAD+ | Arabidopsis thaliana | a step in the Smirnoff-Wheeler pathway, ascorbate biosynthesis in higher plants | L-galactono-1,4-lactone + NADH + H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O81884 | - |
- |
Pisum sativum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pisum sativum |
- |
Arabidopsis thaliana |
ammonium sulfate precipitation, hydrophobic interaction chromatography, anion exchange chromatography, Blue Sepharose affinity chromatography and gel filtration | Pisum sativum |
immobilized metal ion affinity chromatography (Ni2+) and gel filtration | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryonic axis | - |
Pisum sativum | - |
embryonic axis | - |
Arabidopsis thaliana | - |
seedling | - |
Pisum sativum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-fucose + NAD+ | - |
Arabidopsis thaliana | ? | - |
? | |
L-fucose + NAD+ | - |
Arabidopsis thaliana | ? + NADH + H+ | - |
? | |
L-galactose + NAD+ | - |
Pisum sativum | L-galactono-1,4-lactone + NADH + H+ | - |
ir | |
L-galactose + NAD+ | - |
Arabidopsis thaliana | L-galactono-1,4-lactone + NADH + H+ | - |
ir | |
L-galactose + NAD+ | activity with 0.1 mM NADP+ is 6% of that with NAD+, lower activity with L-sorbosone, no activity with D-arabinose and L-fucose | Pisum sativum | L-galactono-1,4-lactone + NADH + H+ | - |
ir | |
L-galactose + NAD+ | NAD+ preferred cofactor, lower activity with L-sorbosone, no activity with D-arabinose, D-galactose, D-glucose and D-mannose | Arabidopsis thaliana | L-galactono-1,4-lactone + NADH + H+ | - |
? | |
L-galactose + NAD+ | a step in the Smirnoff-Wheeler pathway, ascorbate biosynthesis in higher plants | Pisum sativum | L-galactono-1,4-lactone + NADH + H+ | - |
ir | |
L-galactose + NAD+ | a step in the Smirnoff-Wheeler pathway, ascorbate biosynthesis in higher plants | Arabidopsis thaliana | L-galactono-1,4-lactone + NADH + H+ | - |
ir | |
L-galactose + NADP+ | - |
Arabidopsis thaliana | L-galactono-1,4-lactone + NADPH + H+ | - |
? | |
L-gulose + NAD+ | - |
Pisum sativum | ? | - |
? | |
L-gulose + NAD+ | - |
Pisum sativum | ? + NADH + H+ | - |
? | |
L-sorbosone + NAD+ | - |
Arabidopsis thaliana | ? | - |
? | |
additional information | no activity is detectable with D-arabinose, D-galactose, D-glucose and D-mannose | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 42200, SDS-PAGE and gel filtration | Arabidopsis thaliana |
homodimer | 2 * 42200, SDS-PAGE | Arabidopsis thaliana |
homotetramer | 4 * 40000, SDS-PAGE | Pisum sativum |
homotetramer | 4 * 40000, gel filtration and SDS-PAGE, N-terminal sequencing | Pisum sativum |
Synonyms | Comment | Organism |
---|---|---|
L-galactose dehydrogenase | - |
Pisum sativum |
L-galactose dehydrogenase | - |
Arabidopsis thaliana |
L-GalDH | - |
Pisum sativum |
L-GalDH | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pisum sativum |
8.5 | 9 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pisum sativum | |
NAD+ | - |
Arabidopsis thaliana | |
NAD+ | preferred cofactor | Arabidopsis thaliana | |
NAD+ | the activity with 0.1 mM NADP+ is 6% of that with NAD+ | Pisum sativum |
General Information | Comment | Organism |
---|---|---|
metabolism | role in ascorbate biosynthesis | Pisum sativum |
metabolism | role in ascorbate biosynthesis | Arabidopsis thaliana |