Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.316 extracted from

  • Gatzek, S.; Wheeler, G.L.; Smirnoff, N.
    Antisense suppression of L-galactose dehydrogenase in Arabidopsis thaliana provides evidence for its role in ascorbate synthesis and reveals light modulated L-galactose synthesis (2002), Plant J., 30, 541-553.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana
His-tagged version expressed in Escherichia coli BL21(DE3)lysS, expressed in Nicotiana tabacum SRI Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
L-galactono-1,4-lactone uncompetitive Pisum sativum
N-ethylmaleimide 1 mM, 90% inhibition; 90% inhibition with 1 mM Pisum sativum
oxidized glutathione 5 mM, 65% inhibition; 65% inhibition with 5 mM Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0203
-
NAD+ pH 7.5, temperature not specified in the publication Arabidopsis thaliana
0.085
-
L-galactose pH 7.5, temperature not specified in the publication Arabidopsis thaliana
0.43
-
L-galactose pH 7.5, 22°C Pisum sativum
0.43
-
L-galactose pH 7.5, temperature not specified in the publication Pisum sativum
0.59
-
NADP+ pH 7.5, temperature not specified in the publication Arabidopsis thaliana
3.7
-
L-gulose pH 7.5, 22°C Pisum sativum
3.7
-
L-gulose pH 7.5, temperature not specified in the publication Pisum sativum
56.3
-
L-fucose pH 7.5, temperature not specified in the publication Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
4 * 40000, SDS-PAGE Pisum sativum
40000
-
4 * 40000, gel filtration and SDS-PAGE, N-terminal sequencing Pisum sativum
42200
-
2 * 42200, SDS-PAGE and gel filtration Arabidopsis thaliana
42200
-
2 * 42200, SDS-PAGE Arabidopsis thaliana
87500
-
gel filtration Arabidopsis thaliana
87500
-
gel filtration, two peaks of activity at 42400 Da and 87500 Da Arabidopsis thaliana
156000
-
gel filtration Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-galactose + NAD+ Pisum sativum a step in the Smirnoff-Wheeler pathway, ascorbate biosynthesis in higher plants L-galactono-1,4-lactone + NADH + H+
-
ir
L-galactose + NAD+ Arabidopsis thaliana a step in the Smirnoff-Wheeler pathway, ascorbate biosynthesis in higher plants L-galactono-1,4-lactone + NADH + H+
-
ir

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O81884
-
-
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pisum sativum
-
Arabidopsis thaliana
ammonium sulfate precipitation, hydrophobic interaction chromatography, anion exchange chromatography, Blue Sepharose affinity chromatography and gel filtration Pisum sativum
immobilized metal ion affinity chromatography (Ni2+) and gel filtration Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
embryonic axis
-
Pisum sativum
-
embryonic axis
-
Arabidopsis thaliana
-
seedling
-
Pisum sativum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-fucose + NAD+
-
Arabidopsis thaliana ?
-
?
L-fucose + NAD+
-
Arabidopsis thaliana ? + NADH + H+
-
?
L-galactose + NAD+
-
Pisum sativum L-galactono-1,4-lactone + NADH + H+
-
ir
L-galactose + NAD+
-
Arabidopsis thaliana L-galactono-1,4-lactone + NADH + H+
-
ir
L-galactose + NAD+ activity with 0.1 mM NADP+ is 6% of that with NAD+, lower activity with L-sorbosone, no activity with D-arabinose and L-fucose Pisum sativum L-galactono-1,4-lactone + NADH + H+
-
ir
L-galactose + NAD+ NAD+ preferred cofactor, lower activity with L-sorbosone, no activity with D-arabinose, D-galactose, D-glucose and D-mannose Arabidopsis thaliana L-galactono-1,4-lactone + NADH + H+
-
?
L-galactose + NAD+ a step in the Smirnoff-Wheeler pathway, ascorbate biosynthesis in higher plants Pisum sativum L-galactono-1,4-lactone + NADH + H+
-
ir
L-galactose + NAD+ a step in the Smirnoff-Wheeler pathway, ascorbate biosynthesis in higher plants Arabidopsis thaliana L-galactono-1,4-lactone + NADH + H+
-
ir
L-galactose + NADP+
-
Arabidopsis thaliana L-galactono-1,4-lactone + NADPH + H+
-
?
L-gulose + NAD+
-
Pisum sativum ?
-
?
L-gulose + NAD+
-
Pisum sativum ? + NADH + H+
-
?
L-sorbosone + NAD+
-
Arabidopsis thaliana ?
-
?
additional information no activity is detectable with D-arabinose, D-galactose, D-glucose and D-mannose Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 42200, SDS-PAGE and gel filtration Arabidopsis thaliana
homodimer 2 * 42200, SDS-PAGE Arabidopsis thaliana
homotetramer 4 * 40000, SDS-PAGE Pisum sativum
homotetramer 4 * 40000, gel filtration and SDS-PAGE, N-terminal sequencing Pisum sativum

Synonyms

Synonyms Comment Organism
L-galactose dehydrogenase
-
Pisum sativum
L-galactose dehydrogenase
-
Arabidopsis thaliana
L-GalDH
-
Pisum sativum
L-GalDH
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pisum sativum
8.5 9
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pisum sativum
NAD+
-
Arabidopsis thaliana
NAD+ preferred cofactor Arabidopsis thaliana
NAD+ the activity with 0.1 mM NADP+ is 6% of that with NAD+ Pisum sativum

General Information

General Information Comment Organism
metabolism role in ascorbate biosynthesis Pisum sativum
metabolism role in ascorbate biosynthesis Arabidopsis thaliana