Literature summary for 1.1.1.315 extracted from

Wu, Z.; Yang, Y.; Shaw, N.; Bhattacharya, S.; Yan, L.; West, K.; Roth, K.; Noy, N.; Qin, J.; Crabb, J.W.
Mapping the ligand binding pocket in the cellular retinaldehyde binding protein (2003), J. Biol. Chem., 278, 12390-12396.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Hi-5 insect cells	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0025	-	11-cis-retinol-[cellular retinaldehyde binding protein]	wild-type cellular retinaldehyde binding protein, recombinant wild-type RDH5, pH 7.5, temperature not specified in the publication	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q92781	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
11-cis-retinol-[cellular retinaldehyde binding protein] + NAD+	cellular retinaldehyde binding protein CRALBP serves as an 11-cis-retinol acceptor for the enzymatic isomerization of all-trans- to 11-cis-retinol and as a substrate carrier for 11-cis-retinol dehydrogenase RDH5. Altered kinetic parameters are observed for RDH5 oxidation of 11-cis-retinol bound to rCRALBP mutants M222A, M225A, and W244F, supporting impaired substrate carrier function. Data implicate Trp165, Met208, Met222, Met225, and Trp244 as components of the CRALBP ligand binding cavity	Homo sapiens	11-cis-retinal-[cellular retinaldehyde binding protein] + NADH + H+	-	?

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Release 2023.1 (January 2023)