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Literature summary for 1.1.1.315 extracted from

  • Chai, X.; Zhai, Y.; Napoli, J.L.
    cDNA cloning and characterization of a cis-retinol/3alpha-hydroxysterol short-chain dehydrogenase (1997), J. Biol. Chem., 272, 33125-33131.
    View publication on PubMed
Search for more literature for 1.1.1.315

Cloned(Commentary)

Cloned (Comment) Organism
expressed in CHO cells Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
4-Methylpyrazole
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the enzyme exhibits cooperative kinetics for 9-cis-retinol with a K0.5 value of 0.0054 mM and a Hill coefficient of 1.7. The enzyme recognized 11-cis-retinol as substrate with a K0.5 value of 0.0076 and a Hill coefficient of 2.8 Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
9-cis-retinol + NAD+ Mus musculus the multifunctional cis-retinol/3alpha-hydroxysterol short-chain dehydrogenase may catalyze the first step in an enzymatic pathway from 9-cis-retinol to generate the retinoid X receptor ligand 9-cis-retinoic acid and/or may regenerate dihydrotestosterone from its catabolite 5alpha-androstan-3alpha,17beta-diol 9-cis-retinal + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus O54909
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart low mRNA expression Mus musculus
-
kidney mRNA is expressed intensely Mus musculus
-
liver mRNA is expressed intensely Mus musculus
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retina pigment epithelium and sclera, low mRNA expression Mus musculus
-
small intestine low mRNA expression Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-cis-retinol + NAD+ little preference between 9-cis-retinol and 11-cis-retinol. Uses NAD+ as its preferred cofactor Mus musculus 11-cis-retinal + NADH + H+
-
 ?
9-cis-retinol + NAD+ the multifunctional cis-retinol/3alpha-hydroxysterol short-chain dehydrogenase may catalyze the first step in an enzymatic pathway from 9-cis-retinol to generate the retinoid X receptor ligand 9-cis-retinoic acid and/or may regenerate dihydrotestosterone from its catabolite 5alpha-androstan-3alpha,17beta-diol Mus musculus 9-cis-retinal + NADH + H+
-
 ?
9-cis-retinol + NAD+ little preference between 9-cis-retinol and 11-cis-retinol. Uses NAD+ as its preferred cofactor, activity with NADP+ is 4% of the activity with NAD+ Mus musculus 9-cis-retinal + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
cis-retinol/3alpha-hydroxysterol short-chain dehydrogenase multifunctional enzyme Mus musculus
CRAD
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD+ is the preferred cofactor Mus musculus