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Literature summary for 1.1.1.315 extracted from

  • Takahashi, Y.; Moiseyev, G.; Farjo, K.; Ma, J.X.
    Characterization of key residues and membrane association domains in retinol dehydrogenase 10 (2009), Biochem. J., 419, 113-122.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.315

Cloned(Commentary)

Cloned (Comment) Organism
expression in Cos-1 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D169A mutant enzyme completely loses enzymatic activity Homo sapiens
D169N mutant enzyme completely loses enzymatic activity Homo sapiens
G43A/G47A/G49A mutant enzyme completely loses enzymatic activity Homo sapiens
K214A mutant enzyme completely loses enzymatic activity Homo sapiens
K214R mutant enzyme completely loses enzymatic activity Homo sapiens
additional information deletion of the two hydrophobic domains dissociates RDH10 from the membrane and abolishes its activity (mutants DELTA2–23, DELTA293–329 and the double mutant lacking both of these regions) Homo sapiens
S197A mutation does not abolish activity Homo sapiens
S197C mutant enzyme completely loses enzymatic activity Homo sapiens
S197G mutation does not abolish activity Homo sapiens
S197T mutant enzyme completely loses enzymatic activity Homo sapiens
S197V mutant enzyme completely loses enzymatic activity Homo sapiens
Y210A mutant enzyme completely loses enzymatic activity Homo sapiens
Y210F mutant enzyme completely loses enzymatic activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00018
-
 all-trans retinol pH 7.4, 37°, mutynt enzyme S197A Homo sapiens
0.0011
-
 all-trans retinol pH 7.4, 37°C, mutant enzyme S197G Homo sapiens
0.0041
-
 all-trans retinol pH 7.4, 37°C, wild-type enzyme Homo sapiens
0.015
-
 NADPH pH 7.4, 37°C, wild-type enzyme Homo sapiens
0.027
-
 NADP+ pH 7.4, 37°C, wild-type enzyme Homo sapiens
0.036
-
 NAD+ pH 7.4, 37°C, wild-type enzyme Homo sapiens
0.11
-
 NADH pH 7.4, 37°C, wild-type enzyme Homo sapiens
0.57
-
 all-trans retinal pH 7.4, 37°C, wild-type enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8IZV5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans retinol + NAD+ no significant difference in the binding constants of NADP+ and NADPH versus NAD+ and NADH Homo sapiens all-trans-retinal + NADH + H+
-
 r
all-trans retinol + NADP+ no significant difference in the binding constants of NADP+ and NADPH versus NAD+ and NADH Homo sapiens all-trans-retinal + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
RDH10
-
 Homo sapiens
retinol dehydrogenase 10
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+ no significant difference in the binding constants of NADP+ and NADPH versus NAD+ and NADH Homo sapiens
NADH no significant difference in the binding constants of NADP+ and NADPH versus NAD+ and NADH Homo sapiens
NADP+ no significant difference in the binding constants of NADP+ and NADPH versus NAD+ and NADH Homo sapiens
NADPH no significant difference in the binding constants of NADP+ and NADPH versus NAD+ and NADH Homo sapiens