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Literature summary for 1.1.1.315 extracted from
- Activity of human 11-cis-retinol dehydrogenase (Rdh5) with steroids and retinoids and expression of its mRNA in extra-ocular human tissue. (1999), Biochem. J., 338, 23-27.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in CHO cells | Homo sapiens |
| expression in CHO-K1 cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0063 | - |
11-cis-retinol-[cellular retinaldehyde binding protein] | pH 8.0, 37°C | Homo sapiens | |
| 0.0063 | - |
11-cis-retinol | pH 8, 37°C | Homo sapiens |  |
| 0.0066 | - |
9-cis-retinol | pH 8, 37°C | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 11-cis-retinol + NAD+ | Homo sapiens | Rdh5 catalyses 9-cis-retinol metabolism equally efficiently as 11-cis-retinol metabolism. Substrate specificity and expression locus of Rdh5 suggest that it could serve as both an 11-cis-retinol dehydrogenase in the RPE and a 9-cis-retinol dehydrogenase and/or an androgen dehydrogenase outside of the retinal pigment epithelium | 11-cis-retinal + NADH | - |
? | |
| 9-cis-retinol + NAD+ | Homo sapiens | Rdh5 catalyses 9-cis-retinol metabolism equally efficiently as 11-cis-retinol metabolism | 9-cis-retinal + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Homo sapiens | Q92781 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | 17% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| amygdala | 5% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| aorta | 26% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| bladder | 29% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| bone marrow | 11% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| brain | 8% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| caudate nucleus | 9% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| caudate putamen | 9% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| cerebellum | 6% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| cerebral cortex | 5% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| colon | 45% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| colon | 45% of the expression in liver | Homo sapiens | - |
| fetus | fetal brain (5% mRNA expression of Rdh5 compared to adult liver), fetal heart (6% mRNA expression of Rdh5 compared to adultliver), fetal kidney (17% mRNA expression of Rdh5 compared to adult liver), fetal liver (20% mRNA expression of Rdh5 compared to adult liver), fetal spleen (5% mRNA expression of Rdh5 compared to adult liver), fetal thymus (7% mRNA expression of Rdh5 compared to adult liver), fetal lung (14% mRNA expression of Rdh5 compared to liver) | Homo sapiens | - |
| frontal lobe | 3% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| heart | 26% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| hippocampus | 7% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| kidney | 37% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| leukocyte | 9% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| liver | high mRNA expression of Rdh5 | Homo sapiens | - |
| liver | highest expression among extra-ocular tissues tested | Homo sapiens | - |
| lung | 10% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| lymph node | 21% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| mammary gland | 97% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| mammary gland | 97% of the expression in liver | Homo sapiens | - |
| medulla oblongata | 7% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| additional information | mRNA expression is widespread in extra-ocular tissues with human liver and mammary gland showing the most intense signals | Homo sapiens | - |
| occipital lobe | 8% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| ovary | 25% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| pancreas | 28% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| pituitary gland | 20% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| placenta | 9% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| prostate gland | 21% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| salivary gland | 10% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| skeletal muscle | 5% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| small intestine | 39% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| spinal cord | 24% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| spleen | 28% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| stomach | 21% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| substantia nigra | 11% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| subthalamic nucleus | 9% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| temporal lobe | 6% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| testis | 22% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| thalamus | 7% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| thymus | 43% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| thymus | 43% of the expression in liver | Homo sapiens | - |
| thyroid gland | 9% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| trachea | 17% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| uterus | 25% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
| vermiform appendix | 24% mRNA expression of Rdh5 compared to liver | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 11-cis-retinol + NAD+ | - |
Homo sapiens | 11-cis-retinal + NADH | - |
? | |
| 11-cis-retinol + NAD+ | Rdh5 catalyses 9-cis-retinol metabolism equally efficiently as 11-cis-retinol metabolism. Substrate specificity and expression locus of Rdh5 suggest that it could serve as both an 11-cis-retinol dehydrogenase in the RPE and a 9-cis-retinol dehydrogenase and/or an androgen dehydrogenase outside of the retinal pigment epithelium | Homo sapiens | 11-cis-retinal + NADH | - |
? | |
| 11-cis-retinol-[cellular retinaldehyde binding protein] + NAD+ | - |
Homo sapiens | 11-cis-retinal-[cellular retinaldehyde binding protein] + NADH + H+ | - |
? | |
| 13-cis-retinol + NAD+ | - |
Homo sapiens | 13-cis-retinal + NADH | - |
? | |
| 9-cis-retinol + NAD+ | - |
Homo sapiens | 9-cis-retinal + NADH | - |
? | |
| 9-cis-retinol + NAD+ | Rdh5 catalyses 9-cis-retinol metabolism equally efficiently as 11-cis-retinol metabolism | Homo sapiens | 9-cis-retinal + NADH | - |
? | |
| additional information | no significant activity with all-trans-retinol. Rdh5 recognizes 5alpha-androstan-3alpha,17beta-diol (3alpha-adiol) and androsterone as substrates | Homo sapiens | ? | - |
? | |
| additional information | Rdh5 catalyses 9-cis-retinol metabolism equally efficiently as 11-cis-retinol metabolism and recognizes 5alpha-androstan-3alpha,17beta-diol and androsterone as substrates, i.e. 3alpha-hydroxysteroid dehydrogenase activity, but not testosterone, dihydrotestosterone, oestradiol and corticosterone | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 11-cis-retinol dehydrogenase | - |
Homo sapiens |
| RDH5 | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Homo sapiens | |
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