Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Lubrol | the enzyme activity increases in a sigmoidal manner with increasing concentrations, minimum concentration necessary for the increase of enzyme activity is higher than 0.05 mM, effectively eliminates the dye inhibition | Pseudomonas straminea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-pyrone-4,6-dicarboxylic acid | non-competitive inhibition with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde | Pseudomonas straminea | |
Blue dextran | competitive inhibitor with respect to NADP+ | Pseudomonas straminea | |
methoxy Reactive Blue 2 | inhibits the enzyme non-competitively with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde and competitively with respect to NADP+ | Pseudomonas straminea | |
NADH | competitive inhibition with respect to NAD+, non-competitive inhibition with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde | Pseudomonas straminea | |
NADPH | competitive inhibition with respect to NADP, non-competitive inhibition with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde | Pseudomonas straminea | |
Reactive blue 2 | inhibits the enzyme non-competitively with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde and competitively with respect to NADP+ | Pseudomonas straminea | |
Reactive Blue 4 | inhibits the enzyme non-competitively with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde and competitively with respect to NADP+ | Pseudomonas straminea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas straminea | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-carboxy-2-hydroxymuconate semialdehyde hemiacetal + NADP+ = 2-oxo-2H-pyran-4,6-dicarboxylate + NADPH + H+ | ordered bi-bi mechanism | Pseudomonas straminea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ | - |
Pseudomonas straminea | 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH | following intramolecular dehydrogenation to the lactone 2-pyrone-4,6-dicarboxylic acid | ? | |
additional information | interaction with Blue Dextran-2000 and related dyes | Pseudomonas straminea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Pseudomonas straminea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Pseudomonas straminea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00006 | - |
Blue dextran | - |
Pseudomonas straminea | |
0.0002 | - |
Reactive blue 2 | coenzyme NADP+, similar value with coenzyme NAD+ | Pseudomonas straminea | |
0.0002 | - |
methoxy Reactive Blue 2 | coenzyme NADP+, similar value with coenzyme NAD+ | Pseudomonas straminea | |
0.0045 | - |
Reactive Blue 4 | coenzyme NADP+, similar value with coenzyme NAD+ | Pseudomonas straminea | |
0.072 | - |
Reactive blue 2 | - |
Pseudomonas straminea |