Application | Comment | Organism |
---|---|---|
synthesis | application of (S)-1-phenylethanol dehydrogenase for asymmetric reduction of 42 prochiral ketones and 11 beta-keto esters to enantiopure secondary alcohols. The conversions are carried out in a batch reactor using recombinant Escherichia coli as whole-cell catalysts and isopropanol as reaction solvent and cosubstrate for NADH recovery. Ketones are converted to the respective secondary alcohols with excellent enantiomeric excesses and high productivities | Aromatoleum aromaticum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aromatoleum aromaticum |
Crystallization (Comment) | Organism |
---|---|
docking simulation for prochiral ketone and beta-keto ester substrates | Aromatoleum aromaticum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aromatoleum aromaticum | Q5P5I4 | - |
- |
Aromatoleum aromaticum EbN1 | Q5P5I4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2,2-trifluoroacetophenone + NADH + H+ | - |
Aromatoleum aromaticum | (S)-1-phenyl-2,2,2-trifluoroethan-1-ol + NAD+ | 100% enantiomertic excess | ? | |
2,2,2-trifluoroacetophenone + NADH + H+ | - |
Aromatoleum aromaticum EbN1 | (S)-1-phenyl-2,2,2-trifluoroethan-1-ol + NAD+ | 100% enantiomertic excess | ? | |
2,4'-dichloroacetophenone + NADH + H+ | - |
Aromatoleum aromaticum | (S)-2-chloro-1-(4-chloro-phenyl)-ethanol + NAD+ | 100% enantiomeric excess | ? | |
2,4'-dichloroacetophenone + NADH + H+ | - |
Aromatoleum aromaticum EbN1 | (S)-2-chloro-1-(4-chloro-phenyl)-ethanol + NAD+ | 100% enantiomeric excess | ? | |
4'-hydroxyacetophenone + NADH + H+ | - |
Aromatoleum aromaticum | (S)-4-(1-hydroxyethyl)phenol + NAD+ | 90% enantiomeric excess | ? | |
4-acetylbiphenyl + NADH + H+ | - |
Aromatoleum aromaticum | (S)-1-(biphenyl-4-yl)ethanol + NAD+ | 100% enantiomeric excess | ? | |
4-acetylbiphenyl + NADH + H+ | - |
Aromatoleum aromaticum EbN1 | (S)-1-(biphenyl-4-yl)ethanol + NAD+ | 100% enantiomeric excess | ? | |
4-aminoacetophenone + NADH + H+ | - |
Aromatoleum aromaticum | 1-(4-aminophenyl)ethanol + NAD+ | racemic product | ? | |
4-aminoacetophenone + NADH + H+ | - |
Aromatoleum aromaticum EbN1 | 1-(4-aminophenyl)ethanol + NAD+ | racemic product | ? | |
acetophenone + NADH + H+ | - |
Aromatoleum aromaticum | (S)-1-phenylethanol + NAD+ | 100% enantiomeric excess | ? | |
acetophenone + NADH + H+ | - |
Aromatoleum aromaticum EbN1 | (S)-1-phenylethanol + NAD+ | 100% enantiomeric excess | ? | |
methyl 4-fluorobenzoylacetate + NADH + H+ | - |
Aromatoleum aromaticum | (S)-methyl 3-(4-fluorophenyl)-3-hydroxypropanoate + NAD+ | 100% enantiomertic excess | ? |