BRENDA - Enzyme Database
show all sequences of 1.1.1.31

Cloning, expression and characterization of 3-hydroxyisobutyrate dehydrogenase from Pseudomonas denitrificans ATCC 13867

Zhou, S.; Mohan Raj, S.; Ashok, S.; Edwardraja, S.; Lee, S.G.; Park, S.; PLoS ONE 8, e62666 (2013)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
about 182.8% activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
dithiothreitol
about 184.8% activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
EDTA
about 150.9% activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Pseudomonas denitrificans (nom. rej.)
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
complete inhibition at 1 mM
Pseudomonas denitrificans (nom. rej.)
Co2+
about 64.3% residual activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
Cu2+
about 14.5% residual activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
Fe2+
about 9.1% residual activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
Hg2+
complete inhibition at 1 mM
Pseudomonas denitrificans (nom. rej.)
Mg2+
about 88.6% residual activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
Ni2+
about 62.3% residual activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
Zn2+
about 14.5% residual activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.164
-
NAD+
with 3-hydroxypropionic acid as cosubstrate, at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
0.178
-
(S)-3-hydroxyisobutyrate
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
1.055
-
3-hydroxypropionic acid
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
24.18
-
methyl DL-serine
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
25.14
-
methyl (2S)-3-hydroxy-2-methylpropanoate
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
117.5
-
L-serine
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
about 130.7% activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
additional information
not influenced by Na+, K+, NH3+, and Li+
Pseudomonas denitrificans (nom. rej.)
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
x * 30000, SDS-PAGE
Pseudomonas denitrificans (nom. rej.)
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas denitrificans (nom. rej.)
-
-
-
Pseudomonas denitrificans (nom. rej.) ATCC 13867
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni-NTA resin column chromatography
Pseudomonas denitrificans (nom. rej.)
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(S)-3-hydroxyisobutyrate + NAD+
the enzyme is very specific to (S)-3-hydroxyisobutyrate
739465
Pseudomonas denitrificans (nom. rej.)
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
the enzyme is very specific to (S)-3-hydroxyisobutyrate
739465
Pseudomonas denitrificans (nom. rej.) ATCC 13867
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
?
3-hydroxypropionic acid + NAD+
-
739465
Pseudomonas denitrificans (nom. rej.)
malonate semialdehyde + NADH + H+
-
-
-
r
3-hydroxypropionic acid + NAD+
-
739465
Pseudomonas denitrificans (nom. rej.) ATCC 13867
malonate semialdehyde + NADH + H+
-
-
-
r
L-serine + NAD+
-
739465
Pseudomonas denitrificans (nom. rej.)
3-oxo-L-alanine + NADH + H+
-
-
-
r
L-serine + NAD+
-
739465
Pseudomonas denitrificans (nom. rej.) ATCC 13867
3-oxo-L-alanine + NADH + H+
-
-
-
r
methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+
-
739465
Pseudomonas denitrificans (nom. rej.)
methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+
-
-
-
r
methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+
-
739465
Pseudomonas denitrificans (nom. rej.) ATCC 13867
methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+
-
-
-
r
methyl DL-serine + NAD+
-
739465
Pseudomonas denitrificans (nom. rej.)
methyl 3-oxo-DL-alanine + NADH + H+
-
-
-
r
methyl DL-serine + NAD+
-
739465
Pseudomonas denitrificans (nom. rej.) ATCC 13867
methyl 3-oxo-DL-alanine + NADH + H+
-
-
-
r
Subunits
Subunits
Commentary
Organism
?
x * 30000, SDS-PAGE
Pseudomonas denitrificans (nom. rej.)
Synonyms
Synonyms
Commentary
Organism
3HIBDH-IV
-
Pseudomonas denitrificans (nom. rej.)
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Pseudomonas denitrificans (nom. rej.)
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
25
40
more than 80% activity between 25C and 40C
Pseudomonas denitrificans (nom. rej.)
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.62
-
methyl DL-serine
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
6.717
-
NAD+
with 3-hydroxypropionic acid as cosubstrate, at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
10.8
-
3-hydroxypropionic acid
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
35.76
-
methyl (2S)-3-hydroxy-2-methylpropanoate
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
47.55
-
L-serine
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
80.79
-
(S)-3-hydroxyisobutyrate
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Pseudomonas denitrificans (nom. rej.)
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8
10
more than 60% activity between pH 8.0 and 10.0
Pseudomonas denitrificans (nom. rej.)
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
dependent on
Pseudomonas denitrificans (nom. rej.)
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
about 182.8% activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
dithiothreitol
about 184.8% activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
EDTA
about 150.9% activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Pseudomonas denitrificans (nom. rej.)
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
dependent on
Pseudomonas denitrificans (nom. rej.)
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
complete inhibition at 1 mM
Pseudomonas denitrificans (nom. rej.)
Co2+
about 64.3% residual activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
Cu2+
about 14.5% residual activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
Fe2+
about 9.1% residual activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
Hg2+
complete inhibition at 1 mM
Pseudomonas denitrificans (nom. rej.)
Mg2+
about 88.6% residual activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
Ni2+
about 62.3% residual activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
Zn2+
about 14.5% residual activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.164
-
NAD+
with 3-hydroxypropionic acid as cosubstrate, at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
0.178
-
(S)-3-hydroxyisobutyrate
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
1.055
-
3-hydroxypropionic acid
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
24.18
-
methyl DL-serine
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
25.14
-
methyl (2S)-3-hydroxy-2-methylpropanoate
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
117.5
-
L-serine
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
about 130.7% activity at 1 mM
Pseudomonas denitrificans (nom. rej.)
additional information
not influenced by Na+, K+, NH3+, and Li+
Pseudomonas denitrificans (nom. rej.)
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
x * 30000, SDS-PAGE
Pseudomonas denitrificans (nom. rej.)
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA resin column chromatography
Pseudomonas denitrificans (nom. rej.)
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(S)-3-hydroxyisobutyrate + NAD+
the enzyme is very specific to (S)-3-hydroxyisobutyrate
739465
Pseudomonas denitrificans (nom. rej.)
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
the enzyme is very specific to (S)-3-hydroxyisobutyrate
739465
Pseudomonas denitrificans (nom. rej.) ATCC 13867
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
?
3-hydroxypropionic acid + NAD+
-
739465
Pseudomonas denitrificans (nom. rej.)
malonate semialdehyde + NADH + H+
-
-
-
r
3-hydroxypropionic acid + NAD+
-
739465
Pseudomonas denitrificans (nom. rej.) ATCC 13867
malonate semialdehyde + NADH + H+
-
-
-
r
L-serine + NAD+
-
739465
Pseudomonas denitrificans (nom. rej.)
3-oxo-L-alanine + NADH + H+
-
-
-
r
L-serine + NAD+
-
739465
Pseudomonas denitrificans (nom. rej.) ATCC 13867
3-oxo-L-alanine + NADH + H+
-
-
-
r
methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+
-
739465
Pseudomonas denitrificans (nom. rej.)
methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+
-
-
-
r
methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+
-
739465
Pseudomonas denitrificans (nom. rej.) ATCC 13867
methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+
-
-
-
r
methyl DL-serine + NAD+
-
739465
Pseudomonas denitrificans (nom. rej.)
methyl 3-oxo-DL-alanine + NADH + H+
-
-
-
r
methyl DL-serine + NAD+
-
739465
Pseudomonas denitrificans (nom. rej.) ATCC 13867
methyl 3-oxo-DL-alanine + NADH + H+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 30000, SDS-PAGE
Pseudomonas denitrificans (nom. rej.)
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Pseudomonas denitrificans (nom. rej.)
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
25
40
more than 80% activity between 25C and 40C
Pseudomonas denitrificans (nom. rej.)
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.62
-
methyl DL-serine
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
6.717
-
NAD+
with 3-hydroxypropionic acid as cosubstrate, at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
10.8
-
3-hydroxypropionic acid
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
35.76
-
methyl (2S)-3-hydroxy-2-methylpropanoate
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
47.55
-
L-serine
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
80.79
-
(S)-3-hydroxyisobutyrate
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Pseudomonas denitrificans (nom. rej.)
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8
10
more than 60% activity between pH 8.0 and 10.0
Pseudomonas denitrificans (nom. rej.)
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.275
-
methyl DL-serine
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
0.404
-
L-serine
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
1.433
-
methyl (2S)-3-hydroxy-2-methylpropanoate
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
10.23
-
3-hydroxypropionic acid
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
42.44
-
NAD+
with 3-hydroxypropionic acid as cosubstrate, at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
464.9
-
(S)-3-hydroxyisobutyrate
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.275
-
methyl DL-serine
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
0.404
-
L-serine
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
1.433
-
methyl (2S)-3-hydroxy-2-methylpropanoate
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
10.23
-
3-hydroxypropionic acid
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
42.44
-
NAD+
with 3-hydroxypropionic acid as cosubstrate, at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
464.9
-
(S)-3-hydroxyisobutyrate
at 30C and pH 9.0
Pseudomonas denitrificans (nom. rej.)
Other publictions for EC 1.1.1.31
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737620
Park
Structural and biochemical cha ...
Bacillus cereus
Biochem. Biophys. Res. Commun.
474
522-527
2016
-
-
1
-
-
-
-
2
-
-
2
-
-
4
-
-
1
-
-
-
-
-
4
1
1
-
-
-
2
-
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
2
-
-
-
-
1
-
-
-
-
4
1
-
-
-
2
-
1
-
-
-
-
-
-
2
2
737911
Lee
-
3-Hydroxyisobutyrate dehydroge ...
Pseudomonas denitrificans (nom. rej.), Pseudomonas denitrificans (nom. rej.) ATCC 13867
Biotechnol. Bioprocess Eng.
19
1-7
2014
3
-
1
-
-
-
11
5
-
2
1
-
-
3
-
-
1
-
-
-
2
-
11
1
2
1
1
-
5
1
1
-
1
-
-
-
3
-
1
1
-
-
-
-
11
-
5
-
2
1
-
-
-
-
1
-
-
2
-
11
1
1
1
-
5
1
1
-
-
-
-
-
-
5
5
722472
Tasi
Characterization of 3-hydroxyi ...
Homo sapiens
J. Assist. Reprod. Genet.
30
505-512
2013
-
-
-
-
-
-
-
-
2
-
-
1
-
4
-
-
-
-
-
11
-
-
1
-
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
11
-
-
1
-
1
-
-
-
1
-
-
-
2
3
3
2
-
-
739465
Zhou
Cloning, expression and charac ...
Pseudomonas denitrificans (nom. rej.), Pseudomonas denitrificans (nom. rej.) ATCC 13867
PLoS ONE
8
e62666
2013
3
-
1
-
-
-
8
6
-
2
1
-
-
14
-
-
1
-
-
-
-
-
10
1
1
1
1
-
6
1
1
-
1
-
-
-
3
-
1
1
-
-
-
-
8
-
6
-
2
1
-
-
-
-
1
-
-
-
-
10
1
1
1
-
6
1
1
-
-
-
-
-
-
6
6
695710
Yao
The catalytic property of 3-hy ...
Bacillus cereus, Bacillus cereus ATCC 14579
Appl. Biochem. Biotechnol.
160
694-703
2009
1
1
3
-
-
-
3
6
-
2
1
6
-
25
-
-
3
-
-
-
4
1
13
1
2
2
1
2
2
3
2
-
6
-
-
-
1
1
3
6
-
-
-
-
4
-
6
-
2
1
6
-
-
-
3
-
-
4
1
13
1
2
1
2
2
3
2
-
-
-
1
1
-
1
1
688515
Murin
Expression of 3-hydroxyisobuty ...
Rattus norvegicus
J. Neurochem.
105
1176-1186
2008
-
-
-
-
-
-
-
-
2
-
-
1
-
4
-
-
-
-
-
5
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670284
Loupatty
Clinical, biochemical, and mol ...
Homo sapiens
Mol. Genet. Metab.
87
243-248
2006
-
-
1
-
-
-
-
2
-
-
-
-
-
7
-
-
1
-
-
1
3
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
1
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655051
Chowdhury
Cloning and overexpression of ...
Pseudomonas putida, Pseudomonas putida E23
Biosci. Biotechnol. Biochem.
67
438-441
2003
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286550
Hawes
Mammalien 3-hydroxyisobutyrate ...
Oryctolagus cuniculus, Homo sapiens, Rattus norvegicus
Methods Enzymol.
324
218-228
2000
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2
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286551
Chowdhury
3-Hydroxyisobutyrate dehydroge ...
Diutina rugosa, Oryctolagus cuniculus, Pseudomonas putida, Pseudomonas putida E23
Biosci. Biotechnol. Biochem.
60
2043-2047
1996
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7
10
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3
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3
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3
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3
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3
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3
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286552
Hawes
Structural and mechanistic asp ...
Rattus norvegicus
Adv. Exp. Med. Biol.
414
395-402
1996
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1
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10
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286544
Rougraff
Purification and characterizat ...
Oryctolagus cuniculus
J. Biol. Chem.
263
327-331
1988
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2
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1
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6
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1
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1
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286523
Worral
3-Hydroxyisobutyrate dehydroge ...
Luteovulum sphaeroides
Biochem. J.
241
297-300
1987
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2
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4
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286546
Hasegawa
-
Distribution in organisms and ...
Bos taurus, Candida parapsilosis, Diutina rugosa, Corynebacterium hydrocarbons, Tsukamurella paurometabola, Oryctolagus cuniculus, Galactomyces reessii, Micrococcus flavus, Micrococcus luteus, Nocardia lyena, Pseudomonas aeruginosa, Pseudomonas dacunhae, Rhodotorula mucilaginosa, Sus scrofa, Aciculoconidium aculeatum
Agric. Biol. Chem.
45
2899-2901
1981
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16
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4
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16
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3
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16
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1
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4
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16
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286547
Hasegawa
-
Purification, crystallization ...
Diutina rugosa
Agric. Biol. Chem.
45
2805-2814
1981
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3
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286548
De Paul Marshall
Regulation of valine catabolis ...
Pseudomonas putida
J. Bacteriol.
110
1073-1081
1972
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286549
Robinson
The purification and propertie ...
Sus scrofa
J. Biol. Chem.
225
511-521
1956
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3
2
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