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Literature summary for 1.1.1.31 extracted from

  • Park, S.C.; Kim, P.H.; Lee, G.S.; Kang, S.G.; Ko, H.J.; Yoon, S.I.
    Structural and biochemical characterization of the Bacillus cereus 3-hydroxyisobutyrate dehydrogenase (2016), Biochem. Biophys. Res. Commun., 474, 522-527.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.274
-
NAD+ at pH 10.0, temperature not specified in the publication Bacillus cereus
0.652
-
(S)-3-hydroxyisobutyrate at pH 10.0, temperature not specified in the publication Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32700
-
-
Bacillus cereus
65000
-
gel filtration Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxyisobutyrate + NAD+ the enzyme is very specific to (S)-3-hydroxyisobutyrate Bacillus cereus 2-methyl-3-oxopropanoate + NADH + H+
-
?
3-hydroxypropionate + NAD+
-
Bacillus cereus 3-oxopropanoate + NADH + H+
-
?
L-serine + NAD+
-
Bacillus cereus 3-oxo-L-alanine + NADH + H+
-
r
additional information no activity with NADP+, DL-glycerate, (R)-3-hydroxyisobutyrate, L-threonine, D-threonine, and D-serine Bacillus cereus ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 32700, calculated from amino acid sequence Bacillus cereus

Synonyms

Synonyms Comment Organism
HIBADH
-
Bacillus cereus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.1
-
NAD+ at pH 10.0, temperature not specified in the publication Bacillus cereus
24.1
-
(S)-3-hydroxyisobutyrate at pH 10.0, temperature not specified in the publication Bacillus cereus

pH Range

pH Minimum pH Maximum Comment Organism
10 11
-
Bacillus cereus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus cereus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
37.1
-
(S)-3-hydroxyisobutyrate at pH 10.0, temperature not specified in the publication Bacillus cereus
55.1
-
NAD+ at pH 10.0, temperature not specified in the publication Bacillus cereus