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Literature summary for 1.1.1.307 extracted from

  • Nidetzky, B.; Mayr, P.; Hadwiger, P.; Stuetz, A.
    Binding energy and specificity in the catalytic mechanism of yeast aldose reductases (1999), Biochem. J., 344, 101-107 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.307

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 cyclohexanecarboxaldehyde pH 7, 25°C Yamadazyma tenuis
0.5
-
 5,6-dideoxy-D-xylo-hexofuranose pH 7, 25°C Yamadazyma tenuis
0.6
-
 5-fluoro-5-deoxy-D-xylofuranose pH 7, 25°C Yamadazyma tenuis
0.7
-
 6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose pH 7, 25°C Yamadazyma tenuis
0.9
-
 5-deoxy-D-xylofuranose pH 7, 25°C Yamadazyma tenuis
1.2
-
 5,6-dideoxy-5-fluoro-D-glucofuranose pH 7, 25°C Yamadazyma tenuis
1.3
-
 5-deoxy-D-xylofuranose pH 7, 25°C [Candida] intermedia
1.4
-
 5,6-dideoxy-5,6-difluoro-D-glucofuranose pH 7, 25°C Yamadazyma tenuis
1.7
-
 5,6-dideoxy-D-xylohexofuranose pH 7, 25°C [Candida] intermedia
2.4
-
 5-fluoro-5-deoxy-D-xylofuranose pH 7, 25°C [Candida] intermedia
2.5
-
 6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose pH 7, 25°C [Candida] intermedia
2.6
-
 5,6-dideoxy-6-fluoro-D-xylo-hexofuranose pH 7, 25°C Yamadazyma tenuis
4.5
-
 5,6-dideoxy-5,6-difluoro-D-glucofuranose pH 7, 25°C [Candida] intermedia
4.7
-
 oenanthaldehyde pH 7, 25°C [Candida] intermedia
5.4
-
 oenanthaldehyde pH 7, 25°C Yamadazyma tenuis
8.9
-
 5,6-dideoxy-5-fluoro-D-glucofuranose pH 7, 25°C [Candida] intermedia
14.7
-
 5,6-dideoxy-6-fluoro-D-xylohexofuranose pH 7, 25°C [Candida] intermedia
50
-
 D-xylose pH 7, 25°C [Candida] intermedia
64
-
 D-xylose pH 7, 25°C Yamadazyma tenuis
74
-
 D-galactose pH 7, 25°C [Candida] intermedia
228
-
 D-galactose pH 7, 25°C Yamadazyma tenuis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NADH + H+ Yamadazyma tenuis
-
 xylitol + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Yamadazyma tenuis
-
-
-
[Candida] intermedia A0A1L0DQY1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6-dideoxy-5,6-difluoro-D-glucofuranose + NADH + H+
-
 Yamadazyma tenuis ? + NAD+
-
 ?
5,6-dideoxy-5,6-difluoro-D-glucofuranose + NADH + H+
-
 [Candida] intermedia ? + NAD+
-
 ?
5,6-dideoxy-5-fluoro-D-glucofuranose + NADH + H+
-
 Yamadazyma tenuis ? + NAD+
-
 ?
5,6-dideoxy-5-fluoro-D-glucofuranose + NADH + H+
-
 [Candida] intermedia ? + NAD+
-
 ?
5,6-dideoxy-6-fluoro-D-xylo-hexofuranose + NADH + H+
-
 Yamadazyma tenuis ? + NAD+
-
 ?
5,6-dideoxy-6-fluoro-D-xylohexofuranose + NADH + H+
-
 [Candida] intermedia ? + NAD+
-
 ?
5,6-dideoxy-D-xylo-hexofuranose + NADH + H+
-
 Yamadazyma tenuis ? + NAD+
-
 ?
5,6-dideoxy-D-xylohexofuranose + NADH + H+
-
 [Candida] intermedia ? + NAD+
-
 ?
5-azido-5-deoxy-D-xylofuranose + NADH + H+
-
 Yamadazyma tenuis ? + NAD+
-
 ?
5-azido-5-deoxy-D-xylofuranose + NADH + H+
-
 [Candida] intermedia ? + NAD+
-
 ?
5-deoxy-D-xylofuranose + NADH + H+
-
 Yamadazyma tenuis ? + NAD+
-
 ?
5-deoxy-D-xylofuranose + NADPH + H+
-
 [Candida] intermedia ? + NADP+
-
 ?
5-fluoro-5-deoxy-D-xylofuranose + NADH + H+
-
 Yamadazyma tenuis ? + NAD+
-
 ?
5-fluoro-5-deoxy-D-xylofuranose + NADH + H+
-
 [Candida] intermedia ? + NAD+
-
 ?
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose + NADH + H+
-
 Yamadazyma tenuis ? + NAD+
-
 ?
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose + NADH + H+
-
 [Candida] intermedia ? + NAD+
-
 ?
6-azido-5,6-dideoxy-D-xylo-hexofuranose + NADH + H+
-
 Yamadazyma tenuis ? + NAD+
-
 ?
6-azido-5,6-dideoxy-D-xylohexofuranose + NADH + H+
-
 [Candida] intermedia ? + NAD+
-
 ?
cyclohexanecarboxaldehyde + NADH + H+
-
 Yamadazyma tenuis ? + NAD+
-
 ?
cyclohexanecarboxaldehyde + NADH + H+
-
 [Candida] intermedia ? + NAD+
-
 ?
D-galactose + NADH + H+
-
 Yamadazyma tenuis ? + NAD+
-
 ?
D-galactose + NADH + H+
-
 [Candida] intermedia ? + NAD+
-
 ?
D-xylose + NADH + H+
-
 Yamadazyma tenuis xylitol + NAD+
-
 ?
D-xylose + NADPH + H+
-
 [Candida] intermedia xylitol + NADP+
-
 ?
oenanthaldehyde + NADH + H+
-
 Yamadazyma tenuis ? + NAD+
-
 ?
oenanthaldehyde + NADH + H+
-
 [Candida] intermedia ? + NAD+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.6
-
 oenanthaldehyde pH 7, 25°C [Candida] intermedia
5.9
-
 D-galactose pH 7, 25°C [Candida] intermedia
10.5
-
 D-xylose pH 7, 25°C [Candida] intermedia
12
-
 D-galactose pH 7, 25°C Yamadazyma tenuis
12
-
 5,6-dideoxy-5,6-difluoro-D-glucofuranose pH 7, 25°C [Candida] intermedia
13
-
 5,6-dideoxy-5-fluoro-D-glucofuranose pH 7, 25°C [Candida] intermedia
13.5
-
 5-deoxy-D-xylofuranose pH 7, 25°C [Candida] intermedia
14
-
 D-xylose pH 7, 25°C Yamadazyma tenuis
14
-
 6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose pH 7, 25°C [Candida] intermedia
14
-
 5,6-dideoxy-D-xylohexofuranose pH 7, 25°C [Candida] intermedia
15.5
-
 5-fluoro-5-deoxy-D-xylofuranose pH 7, 25°C [Candida] intermedia
16
-
 6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose pH 7, 25°C Yamadazyma tenuis
18
-
 5-deoxy-D-xylofuranose pH 7, 25°C Yamadazyma tenuis
18
-
 5,6-dideoxy-5,6-difluoro-D-glucofuranose pH 7, 25°C Yamadazyma tenuis
18
-
 5,6-dideoxy-5-fluoro-D-glucofuranose pH 7, 25°C Yamadazyma tenuis
18
-
 5,6-dideoxy-6-fluoro-D-xylo-hexofuranose pH 7, 25°C Yamadazyma tenuis
18
-
 5,6-dideoxy-D-xylo-hexofuranose pH 7, 25°C Yamadazyma tenuis
19
-
 5-fluoro-5-deoxy-D-xylofuranose pH 7, 25°C Yamadazyma tenuis
20
-
 cyclohexanecarboxaldehyde pH 7, 25°C Yamadazyma tenuis
21
-
 oenanthaldehyde pH 7, 25°C Yamadazyma tenuis
21
-
 5,6-dideoxy-6-fluoro-D-xylohexofuranose pH 7, 25°C [Candida] intermedia

Cofactor

Cofactor Comment Organism Structure
NADH enzyme has dual coenzyme specificity, accepting both NADH and NADPH Yamadazyma tenuis
NADH enzyme has dual coenzyme specificity, accepting both NADH and NADPH [Candida] intermedia
NADPH enzyme has dual coenzyme specificity, accepting both NADH and NADPH Yamadazyma tenuis
NADPH enzyme has dual coenzyme specificity, accepting both NADH and NADPH [Candida] intermedia

General Information

General Information Comment Organism
metabolism derivatives of D-xylose and D-glucose, in which the hydroxy groups at C-5, and C-5 and C-6 are replaced by fluorine, hydrogen and azide are reduced with up to 3000fold increased catalytic efficiencies. Azide introduced at C-5 or C-6 destabilizes the transition state of reduction of the corresponding hydrogen-substituted aldoses by approx. 4 kJ/mol Yamadazyma tenuis
metabolism derivatives of D-xylose and D-glucose, in which the hydroxy groups at C-5, and C-5 and C-6 are replaced by fluorine, hydrogen and azide are reduced with up to 3000fold increased catalytic efficiencies. Azide introduced at C-5 or C-6 destabilizes the transition state of reduction of the corresponding hydrogen-substituted aldoses by approx. 4 kJ/mol [Candida] intermedia
physiological function Candida intermedia produces two different isoforms. Isoform I is strictly specific for NADPH, isoform II shows similar specificity constants for NADPH and NADH [Candida] intermedia

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.053
-
 D-galactose pH 7, 25°C Yamadazyma tenuis
0.08
-
 D-galactose pH 7, 25°C [Candida] intermedia
0.15
-
 6-azido-5,6-dideoxy-D-xylohexofuranose pH 7, 25°C [Candida] intermedia
0.21
-
 D-xylose pH 7, 25°C [Candida] intermedia
0.22
-
 D-xylose pH 7, 25°C Yamadazyma tenuis
0.35
-
 5-azido-5-deoxy-D-xylofuranose pH 7, 25°C [Candida] intermedia
0.98
-
 oenanthaldehyde pH 7, 25°C [Candida] intermedia
1.08
-
 cyclohexanecarboxaldehyde pH 7, 25°C [Candida] intermedia
1.43
-
 5,6-dideoxy-6-fluoro-D-xylohexofuranose pH 7, 25°C [Candida] intermedia
1.46
-
 5,6-dideoxy-5-fluoro-D-glucofuranose pH 7, 25°C [Candida] intermedia
1.7
-
 5-azido-5-deoxy-D-xylofuranose pH 7, 25°C Yamadazyma tenuis
2.67
-
 5,6-dideoxy-5,6-difluoro-D-glucofuranose pH 7, 25°C [Candida] intermedia
3.9
-
 oenanthaldehyde pH 7, 25°C Yamadazyma tenuis
5.6
-
 6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose pH 7, 25°C [Candida] intermedia
6.46
-
 5-fluoro-5-deoxy-D-xylofuranose pH 7, 25°C [Candida] intermedia
6.9
-
 5,6-dideoxy-6-fluoro-D-xylo-hexofuranose pH 7, 25°C Yamadazyma tenuis
7.14
-
 cyclohexanecarboxaldehyde pH 7, 25°C Yamadazyma tenuis
8.24
-
 5,6-dideoxy-D-xylohexofuranose pH 7, 25°C [Candida] intermedia
10.38
-
 5-deoxy-D-xylofuranose pH 7, 25°C [Candida] intermedia
12.86
-
 5,6-dideoxy-5,6-difluoro-D-glucofuranose pH 7, 25°C Yamadazyma tenuis
15
-
 5,6-dideoxy-5-fluoro-D-glucofuranose pH 7, 25°C Yamadazyma tenuis
20
-
 5-deoxy-D-xylofuranose pH 7, 25°C Yamadazyma tenuis
22.86
-
 6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose pH 7, 25°C Yamadazyma tenuis
31.7
-
 5-fluoro-5-deoxy-D-xylofuranose pH 7, 25°C Yamadazyma tenuis
36
-
 5,6-dideoxy-D-xylo-hexofuranose pH 7, 25°C Yamadazyma tenuis
297
-
 NADH pH 7, 25°C [Candida] intermedia
548
-
 NADPH pH 7, 25°C [Candida] intermedia