Application | Comment | Organism |
---|---|---|
synthesis | the microalga Chlorella sorokiniana and provide a target for genetic engineering to improve D-xylose utilization for microalgal lipid production | Chlorella sorokiniana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NADP+ | Chlorella sorokiniana | - |
D-xylose + NADPH + H+ | - |
r | |
xylitol + NADP+ | Chlorella sorokiniana UTEX 1602 | - |
D-xylose + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorella sorokiniana | - |
- |
- |
Chlorella sorokiniana UTEX 1602 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Chlorella sorokiniana | - |
additional information | D-xylose utilization by the oleaginous microalga Chlorella sorokiniana. Compared with the culture in the dark, the consumption of D-xylose increased 2fold under light | Chlorella sorokiniana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.021 | - |
crude enzyme extract, induced cells, pH 6.0, 40°C | Chlorella sorokiniana |
0.027 | - |
crude enzyme extract, induced cells, pH 6.0, 70°C | Chlorella sorokiniana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADH + H+ | - |
Chlorella sorokiniana | xylitol + NAD+ | - |
r | |
D-xylose + NADH + H+ | - |
Chlorella sorokiniana UTEX 1602 | xylitol + NAD+ | - |
r | |
D-xylose + NADPH + H+ | preferred substrates | Chlorella sorokiniana | xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | preferred substrates | Chlorella sorokiniana UTEX 1602 | xylitol + NADP+ | - |
r | |
xylitol + NADP+ | - |
Chlorella sorokiniana | D-xylose + NADPH + H+ | - |
r | |
xylitol + NADP+ | - |
Chlorella sorokiniana UTEX 1602 | D-xylose + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NAD(P)H-linked xylose reductase | - |
Chlorella sorokiniana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Chlorella sorokiniana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Chlorella sorokiniana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | about 90% of maximal activity at pH 5.5, 60% at pH 9.0, and maximal activity at pH 6.0 | Chlorella sorokiniana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme uses both NADPH and NADH but prefers NADPH, the activity with NADPH is about 5fold higher than that with NADH | Chlorella sorokiniana | |
NAD+ | - |
Chlorella sorokiniana | |
NADH | - |
Chlorella sorokiniana | |
NADH | 5fold higher activity with NADPH than with NADH | Chlorella sorokiniana | |
NADP+ | - |
Chlorella sorokiniana | |
NADPH | - |
Chlorella sorokiniana | |
NADPH | 5fold higher activity with NADPH than with NADH | Chlorella sorokiniana |
Organism | Comment | Expression |
---|---|---|
Chlorella sorokiniana | D-xylose uptake induces the enzyme, together with the NADP+-linked xylitol dehydrogenase, EC 1.1.1.10 | up |
Chlorella sorokiniana | induction by presence of D-xylose in culture medium | up |
General Information | Comment | Organism |
---|---|---|
metabolism | D-glucose-induced algal cells exhibit a remarkably increased D-xylose uptake rate. The uptake of D-xylose activates the related metabolic pathway, and the activities of a NAD(P)H-linked xylose reductase XR and NADP+-linked xylitol dehydrogenase XDH are detected in C. sorokiniana. Compared with the culture in the dark, the consumption of D-xylose increases 2fold under light but decreases to the same level with addition of 3-(3,4-dichlorophenyl)-1,1-dimethylurea | Chlorella sorokiniana |
additional information | the transportation of D-xylose across the cell membrane of Chlorella sorokiniana is realized by an inducible hexose symporter. The uptake of D-xylose subsequently activates the expression of key catalytic enzymes that enables D-xylose entering central metabolism | Chlorella sorokiniana |