Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | 1 mg/ml, 20% increase of activity | Candida tropicalis | |
DTT | 1 mM, 27% increase of activity | Candida tropicalis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3), subloned into the pYES2 vector and transformed into Saccharomyces cerevisiae W303-1A | Candida tropicalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0455 | - |
NADPH | pH 5.5, 45°C | Candida tropicalis | |
0.1619 | - |
NADH | pH 5.5, 45°C | Candida tropicalis | |
31.5 | - |
D-xylose | pH 5.5, 45°C | Candida tropicalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no increase in activity in presence of 1 mM NaCl and 1 mM MgSO4 | Candida tropicalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37100 | - |
x * 37100, SDS-PAGE | Candida tropicalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADH + H+ | Candida tropicalis | - |
xylitol + NAD+ | - |
? | |
D-xylose + NADH + H+ | Candida tropicalis SCTCC 300249 | - |
xylitol + NAD+ | - |
? | |
D-xylose + NADPH + H+ | Candida tropicalis | key enzyme in xylose metabolism | xylitol + NADP+ | - |
? | |
D-xylose + NADPH + H+ | Candida tropicalis SCTCC 300249 | key enzyme in xylose metabolism | xylitol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida tropicalis | A9QVV8 | SCTCC 300249 | - |
Candida tropicalis SCTCC 300249 | A9QVV8 | SCTCC 300249 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Candida tropicalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
251.5 | - |
cofactor: NADPH | Candida tropicalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinose + NADPH + H+ | about 60% of the activity compared to D-xylose | Candida tropicalis | ? | - |
? | |
D-arabinose + NADPH + H+ | about 60% of the activity compared to D-xylose | Candida tropicalis SCTCC 300249 | ? | - |
? | |
D-galactose + NADPH + H+ | about 70% of the activity compared to D-xylose | Candida tropicalis | ? | - |
? | |
D-glucose + NADPH + H+ | about 15% of the activity compared to D-xylose | Candida tropicalis | ? | - |
? | |
D-ribose + NADPH + H+ | about 90% of the activity compared to D-xylose | Candida tropicalis | ? | - |
? | |
D-xylose + NADH + H+ | - |
Candida tropicalis | xylitol + NAD+ | - |
? | |
D-xylose + NADH + H+ | dual coenzyme specificity, Km for NADPH: 0.0455 mM, Km for NADH: 0.162 mM | Candida tropicalis | xylitol + NAD+ | - |
? | |
D-xylose + NADH + H+ | - |
Candida tropicalis SCTCC 300249 | xylitol + NAD+ | - |
? | |
D-xylose + NADH + H+ | dual coenzyme specificity, Km for NADPH: 0.0455 mM, Km for NADH: 0.162 mM | Candida tropicalis SCTCC 300249 | xylitol + NAD+ | - |
? | |
D-xylose + NADPH + H+ | key enzyme in xylose metabolism | Candida tropicalis | xylitol + NADP+ | - |
? | |
D-xylose + NADPH + H+ | dual coenzyme specificity, Km for NADPH: 0.0455 mM, Km for NADH: 0.162 mM | Candida tropicalis | xylitol + NADP+ | - |
? | |
D-xylose + NADPH + H+ | key enzyme in xylose metabolism | Candida tropicalis SCTCC 300249 | xylitol + NADP+ | - |
? | |
D-xylose + NADPH + H+ | dual coenzyme specificity, Km for NADPH: 0.0455 mM, Km for NADH: 0.162 mM | Candida tropicalis SCTCC 300249 | xylitol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37100, SDS-PAGE | Candida tropicalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Candida tropicalis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | 30°C: about 70% of maximal activity, 60°C: about 45% of maximal activity | Candida tropicalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 h, 80% loss of activity, half-life is around 15 min | Candida tropicalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
240 | - |
D-xylose | - |
Candida tropicalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Candida tropicalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | pH 4.0: about 55% of maximal activity, pH 6.5: about 60% of maximal activity | Candida tropicalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | dual coenzyme specificity, Km for NADPH: 0.0455 mM, Km for NADH: 0.162 mM | Candida tropicalis | |
NADPH | dual coenzyme specificity, Km for NADPH: 0.0455 mM, Km for NADH: 0.162 mM | Candida tropicalis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
27430 | - |
D-xylose | pH 5.5, 45°C | Candida tropicalis |