Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | 1 mg/ml, 30% activation | Neurospora crassa | |
EDTA | 1 mM, 5% activation | Neurospora crassa |
Application | Comment | Organism |
---|---|---|
synthesis | this enzyme is one of the most active xylose reductases and may be used for the in vitro production of xylitol | Neurospora crassa |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a His6-tagged fusion protein in high yield | Neurospora crassa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect 0.1% w/v sodium azide | Neurospora crassa | |
NaCl | 50 mM, 25% inhibition | Neurospora crassa | |
sodium phosphite | 200 mM, 37% inhibition | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
NADPH | pH 6.3, 25°C | Neurospora crassa | |
0.016 | - |
NADH | pH 6.3, 25°C | Neurospora crassa | |
34 | - |
D-xylose | pH 6.3, 25°C, cosubstrate: NADPH | Neurospora crassa | |
37 | - |
D-xylose | pH 6.3, 25°C, cosubstrate: NADH | Neurospora crassa | |
40 | - |
L-arabinose | pH 6.3, 25°C | Neurospora crassa | |
70 | - |
D-ribose | pH 6.3, 25°C | Neurospora crassa | |
180 | - |
D-galactose | pH 6.3, 25°C | Neurospora crassa | |
360 | - |
D-glucose | pH 6.3, 25°C | Neurospora crassa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect: 1 mM CuSO4 | Neurospora crassa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38400 | - |
2 * 38400, SDS-PAGE | Neurospora crassa |
53000 | - |
gel filtration | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Neurospora crassa |
Storage Stability | Organism |
---|---|
4°C, stable for several months | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + NADPH + H+ | catalytic efficiency is 9.1% of the catalytic efficiency for D-xylose | Neurospora crassa | ? | - |
? | |
D-glucose + NADPH + H+ | catalytic efficiency is 3.3% of the catalytic efficiency for D-xylose | Neurospora crassa | ? | - |
? | |
D-ribose + NADPH + H+ | catalytic efficiency is 41% of the catalytic efficiency for D-xylose | Neurospora crassa | ? | - |
? | |
D-xylose + NADH + H+ | catalytic efficiency for NADPH is more than 100fold higher than the catalytic efficiency for NADH | Neurospora crassa | xylitol + NAD+ | - |
? | |
D-xylose + NADPH + H+ | catalytic efficiency for NADPH is more than 100fold higher than the catalytic efficiency for NADH | Neurospora crassa | xylitol + NADP+ | - |
? | |
L-arabinose + NADPH + H+ | catalytic efficiency is 41% of the catalytic efficiency for D-xylose | Neurospora crassa | L-arabinitol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38400, SDS-PAGE | Neurospora crassa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 55 | - |
Neurospora crassa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 65 | 25°C: about 55% of maximal activity, 65°C: about 65% of maximal activity | Neurospora crassa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
21 | - |
at room temperature stable for more than 1 month | Neurospora crassa |
40 | - |
half-life: 94 min | Neurospora crassa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
310 | - |
NADH | pH 6.3, 25°C | Neurospora crassa | |
310 | - |
D-xylose | pH 6.3, 25°C, cosubstrate: NADH | Neurospora crassa | |
1320 | - |
D-glucose | pH 6.3, 25°C | Neurospora crassa | |
1800 | - |
D-galactose | pH 6.3, 25°C | Neurospora crassa | |
1800 | - |
L-arabinose | pH 6.3, 25°C | Neurospora crassa | |
3120 | - |
D-ribose | pH 6.3, 25°C | Neurospora crassa | |
3600 | - |
NADPH | pH 6.3, 25°C | Neurospora crassa | |
3600 | - |
D-xylose | pH 6.3, 25°C, cosubstrate: NADPH | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Neurospora crassa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | pH 4.0: about 40% of maximal activity, pH 7.0: about 50% of maximal activity | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | catalytic efficiency for NADPH is more than 100fold higher than the catalytic efficiency for NADH | Neurospora crassa | |
NADPH | catalytic efficiency for NADPH is more than 100fold higher than the catalytic efficiency for NADH | Neurospora crassa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
D-glucose | pH 6.3, 25°C | Neurospora crassa | |
0.16 | - |
D-galactose | pH 6.3, 25°C | Neurospora crassa | |
0.75 | - |
L-arabinose | pH 6.3, 25°C | Neurospora crassa | |
0.75 | - |
D-ribose | pH 6.3, 25°C | Neurospora crassa | |
1.8 | - |
D-xylose | pH 6.3, 25°C, cosubstrate: NADPH | Neurospora crassa | |
33.3 | - |
NADPH | pH 6.3, 25°C | Neurospora crassa |