KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0173 | - |
mycothiol | pH 8.7, 30°C | Mycolicibacterium smegmatis | |
0.354 | - |
NAD+ | pH 8.7, 30°C | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38263 | - |
x * 38263, electrospray mass spectrometry | Mycolicibacterium smegmatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Mycolicibacterium smegmatis mc2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycolicibacterium smegmatis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Mycolicibacterium smegmatis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
22.64 | - |
- |
Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-hydroxymethylmycothiol + NAD+ | rapid-equilibrium ordered mechanism | Mycolicibacterium smegmatis | formic acid + mycothiol + NADH + ? | - |
? | |
S-hydroxymethylmycothiol + NAD+ | rapid-equilibrium ordered mechanism | Mycolicibacterium smegmatis mc2 | formic acid + mycothiol + NADH + ? | - |
? | |
S-nitrosomycothiol + NADH | decomposition with a sequential mechanism, enzyme reduces the nitroso group to the oxidation level of nitroxyl | Mycolicibacterium smegmatis | ? | - |
? | |
S-nitrosomycothiol + NADH | decomposition with a sequential mechanism, enzyme reduces the nitroso group to the oxidation level of nitroxyl | Mycolicibacterium smegmatis mc2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38263, electrospray mass spectrometry | Mycolicibacterium smegmatis |
Synonyms | Comment | Organism |
---|---|---|
MSH-dependent formaldehyde dehydrogenase | - |
Mycolicibacterium smegmatis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | optimal in Na-diphosphate buffer, buffers containing amino groups give poor activity, possibly because of Schiff base formation with formaldehyde | Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mycolicibacterium smegmatis |