Activating Compound | Comment | Organism | Structure |
---|---|---|---|
factor | unidentified factor that is required for maximal activity with formaldehyde as substrate, only 4% activity in its absence, oxidation of alcohols do not require the factor | Amycolatopsis methanolica | |
methanol | can replace an unidentified activating factor | Amycolatopsis methanolica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 80% inhibition at 5 mM | Amycolatopsis methanolica | |
2-(cyclohexylamino)ethanesulfonic acid-NaOH | 25 mM at pH 9.0: 80% of the activity compared to 0.1 M diphosphate, pH 9.0 | Amycolatopsis methanolica | |
acetaldehyde | 15% inhibition at 1 mM | Amycolatopsis methanolica | |
Cu2+ | complete inhibition at 1 mM | Amycolatopsis methanolica | |
glycine-NaOH | 100 mM at pH 9.0: 40% of the activity compared to 0.1 M diphosphate, pH 9.0 | Amycolatopsis methanolica | |
Hg2+ | complete inhibition at 0.1 mM | Amycolatopsis methanolica | |
KCN | 55% inhibition at 2 mM | Amycolatopsis methanolica | |
Na2B4O7-HCl | 100 mM at pH 9.0: no activity | Amycolatopsis methanolica | |
Tris-HCl | 50 mM at pH 9.0: 80% of the activity compared to 0.1 M diphosphate, pH 9.0 | Amycolatopsis methanolica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
1-Octanol | - |
Amycolatopsis methanolica | |
1.7 | - |
12-Hydroxydodecanoic acid | - |
Amycolatopsis methanolica | |
3.4 | - |
1-Hexanol | - |
Amycolatopsis methanolica | |
9.6 | - |
formaldehyde | - |
Amycolatopsis methanolica | |
26.5 | - |
1-Pentanol | - |
Amycolatopsis methanolica | |
84 | - |
1-butanol | - |
Amycolatopsis methanolica | |
324 | - |
1-propanol | - |
Amycolatopsis methanolica | |
343 | - |
ethanol | - |
Amycolatopsis methanolica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 6 atoms Zn/enzyme molecule | Amycolatopsis methanolica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
3 * 40000, SDS-PAGE | Amycolatopsis methanolica |
120000 | - |
gel filtration | Amycolatopsis methanolica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + 1-O-(2'-[N-acetyl-L-cysteinyl]amido-2'-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol + NAD+ | Amycolatopsis methanolica | - |
S-formylmycothiol + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis methanolica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Amycolatopsis methanolica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | induction of enzyme and factor synthesis only if cells grown in methanol-containing medium | Amycolatopsis methanolica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.03 | - |
cell free extract, in the absence of an unknown activating factor | Amycolatopsis methanolica |
0.08 | - |
cell free extract, in the presence of an unknown activating factor | Amycolatopsis methanolica |
6.9 | - |
substrate: ethanol | Amycolatopsis methanolica |
15 | - |
substrate: formaldehyde | Amycolatopsis methanolica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + NAD+ | - |
Amycolatopsis methanolica | n-butanal + NADH | - |
? | |
1-hexanol + NAD+ | - |
Amycolatopsis methanolica | hexanal + NADH + H+ | - |
r | |
1-octanol + NAD+ | - |
Amycolatopsis methanolica | octanal + NADH + H+ | - |
r | |
1-pentanol + NAD+ | - |
Amycolatopsis methanolica | pentanal + NADH + H+ | - |
r | |
1-propanol + NAD+ | - |
Amycolatopsis methanolica | propanal + NADH + H+ | - |
r | |
12-hydroxydodecanoic acid + NADH | - |
Amycolatopsis methanolica | ? + NAD+ | - |
? | |
ethanol + NAD+ | - |
Amycolatopsis methanolica | ethanal + NADH + H+ | - |
r | |
formaldehyde + 1-O-(2'-[N-acetyl-L-cysteinyl]amido-2'-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol + NAD+ | - |
Amycolatopsis methanolica | S-formylmycothiol + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 40000, SDS-PAGE | Amycolatopsis methanolica |
Synonyms | Comment | Organism |
---|---|---|
FD-FA1DH | - |
Amycolatopsis methanolica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
formaldehyde as substrate | Amycolatopsis methanolica |
10.2 | - |
alcohols as substrates | Amycolatopsis methanolica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Amycolatopsis methanolica |