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Literature summary for 1.1.1.304 extracted from
- Purification and classification of diacetyl-reducing enzymes from Staphylococcus aureus (1988), Biochem. J., 251, 461-466.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diacetyl | substrate inhibition at concentrations above 80-90 mM | Staphylococcus aureus |  |
| ethyl pyruvate | substrate inhibition at concentrations above 80-90 mM | Staphylococcus aureus | |
| Methyl pyruvate | substrate inhibition at concentrations above 80-90 mM | Staphylococcus aureus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
NADH | 25°C, pH 6.0, cosubstrate 2,3-pentanedione | Staphylococcus aureus | |
| 0.045 | - |
NADH | 25°C, pH 6.0, cosubstrate diacetyl | Staphylococcus aureus | |
| 0.095 | - |
NADH | 25°C, pH 6.0, cosubstrate methyl pyruvate | Staphylococcus aureus | |
| 0.11 | - |
NADH | 25°C, pH 6.0, cosubstrate ethyl pyruvate | Staphylococcus aureus | |
| 6 | - |
2,3-Pentanedione | 25°C, pH 6.0 | Staphylococcus aureus | |
| 15 | - |
diacetyl | 25°C, pH 6.0 | Staphylococcus aureus | |
| 16 | - |
Methyl pyruvate | 25°C, pH 6.0 | Staphylococcus aureus | |
| 24 | - |
ethyl pyruvate | 25°C, pH 6.0 | Staphylococcus aureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
gel filtration | Staphylococcus aureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-pentanedione + beta-NADH + H+ | - |
Staphylococcus aureus | L-3-hydroxy-2-pentanone + beta-NAD+ | - |
ir | |
| diacetyl + beta-NADH + H+ | 86.9% of the activity with pentane-2,3-dione | Staphylococcus aureus | (S)-acetoin + beta-NAD+ | - |
ir | |
| ethyl pyruvate + beta-NADH + H+ | 38.4% of the activity with pentane-2,3-dione | Staphylococcus aureus | ? + beta-NAD+ | - |
ir | |
| methyl pyruvate + beta-NADH + H+ | 22.8% of the activity with pentane-2,3-dione | Staphylococcus aureus | ? + beta-NAD+ | - |
ir | |
| additional information | no activity with alpha-NADH or NADPH | Staphylococcus aureus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 68000, SDS-PAGE | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Staphylococcus aureus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with alpha-NADH or NADPH | Staphylococcus aureus | |
| NADH | beta-NADH | Staphylococcus aureus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 150 | - |
ethyl pyruvate | 25°C, pH 6.0 | Staphylococcus aureus | |
| 150 | - |
Methyl pyruvate | 25°C, pH 6.0 | Staphylococcus aureus | |
| 300 | - |
diacetyl | 25°C, pH 6.0 | Staphylococcus aureus | |
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