Cloned (Comment) | Organism |
---|---|
recombinant enzyme expression in Escherichia coli | Neisseria gonorrhoeae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacetyl + NADH + H+ | Neisseria gonorrhoeae | - |
(R)-acetoin + NAD+ | - |
ir | |
diacetyl + NADH + H+ | Neisseria gonorrhoeae FA1090 | - |
(R)-acetoin + NAD+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria gonorrhoeae | - |
- |
- |
Neisseria gonorrhoeae FA1090 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacetyl + NADH + H+ | - |
Neisseria gonorrhoeae | (R)-acetoin + NAD+ | - |
ir | |
diacetyl + NADH + H+ | 60% activity compared to main activity of 2,3-butanediol dehydrogenase, i.e. oxidation of (2R,3R)-2,3-butanediol and reduction of (R)-acetoin, EC 1.1.1.4 | Neisseria gonorrhoeae | (R)-acetoin + NAD+ | - |
ir | |
diacetyl + NADH + H+ | - |
Neisseria gonorrhoeae FA1090 | (R)-acetoin + NAD+ | - |
ir | |
diacetyl + NADH + H+ | 60% activity compared to main activity of 2,3-butanediol dehydrogenase, i.e. oxidation of (2R,3R)-2,3-butanediol and reduction of (R)-acetoin, EC 1.1.1.4 | Neisseria gonorrhoeae FA1090 | (R)-acetoin + NAD+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Neisseria gonorrhoeae |
Synonyms | Comment | Organism |
---|---|---|
acetoin/diacetyl reductase | - |
Neisseria gonorrhoeae |
gutB | - |
Neisseria gonorrhoeae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Neisseria gonorrhoeae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Neisseria gonorrhoeae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Neisseria gonorrhoeae |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is a 2,3-butanediol dehydrogenase (BDH, EC 1.1.1.4) and also a acetoin/diacetyl reductase, that catalyzes the stereospecific oxidation of (2R,3R)-2,3-butanediol and the reverse reduction of (R)-acetoin to (2R,3R)-2,3-butanediol, as well as the reduction of diacetyl to (R)-acetoin. It is a pivotal enzyme for the formation of 2,3-butanediol (2,3-BD), a chiral compound with potential roles in the virulence of certain pathogens | Neisseria gonorrhoeae |