Cloned (Comment) | Organism |
---|---|
expression in HEK-293 cell | Danio rerio |
expression in HEK-293 cell | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
M144G | gain-of-function mutant, enables estrone to bind and be reduced as an additional substrate | Homo sapiens |
M146G | mutation in isoform prRDH1, gain-of-function mutant, enables estrone to bind and be reduced as an additional substrate | Danio rerio |
M147G | mutation in isoform prRDH2, gain-of-function mutant, enables estrone to bind and be reduced as an additional substrate | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00126 | - |
all-trans-retinal | wild-type, pH 7.3, 37°C | Homo sapiens | |
0.0017 | - |
all-trans-retinal | mutant M146G, pH 7.3, 37°C | Danio rerio | |
0.0023 | - |
all-trans-retinal | mutant M144G, pH 7.3, 37°C | Homo sapiens | |
0.003 | - |
all-trans-retinal | mutant M147G, pH 7.3, 37°C | Danio rerio | |
0.0031 | - |
all-trans-retinal | wild-type isoform prRDH1, pH 7.3, 37°C | Danio rerio | |
0.0044 | - |
all-trans-retinal | wild-type isoform prRDH1, pH 7.3, 37°C | Danio rerio | |
0.0096 | - |
estrone | mutant M144G, pH 7.3, 37°C | Homo sapiens | |
0.0233 | - |
estrone | mutant M147G, pH 7.3, 37°C | Danio rerio | |
0.0307 | - |
estrone | mutant M146G, pH 7.3, 37°C | Danio rerio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | - |
- |
- |
Homo sapiens | Q9NYR8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans-retinal + NADH + H+ | - |
Danio rerio | all-trans-retinol + NAD+ | - |
? | |
all-trans-retinal + NADH + H+ | - |
Homo sapiens | all-trans-retinol + NAD+ | - |
? | |
estrone + NADH + H+ | no substrate for wild-type isoforms prRDH1 and prRDH2, but substrate for mutants M146G of prRDH1 and M147G of prRDH2 | Danio rerio | estradiol + NAD+ | - |
? | |
estrone + NADH + H+ | no substrate for wild-type, but substrate for mutant M144G | Homo sapiens | estradiol + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
photoreceptor-associated retinol dehydrogenase | - |
Danio rerio |
photoreceptor-associated retinol dehydrogenase | - |
Homo sapiens |
prRDH | - |
Danio rerio |
prRDH | - |
Homo sapiens |