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Literature summary for 1.1.1.3 extracted from

  • Shen, S.; Zhu, Y.; Fang, L.; Xu, J.
    Heterologous expression and characterization of L200F/D215K mutant of homoserine dehydrogenase from Corynebacterium pekinense AS1.299 (2014), Wei Sheng Wu Xue Bao, 54, 1178-1184.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21 Corynebacterium pekinense

Protein Variants

Protein Variants Comment Organism
L200F site-directed mutagenesis, compared to mutant L200F, the double mutant shows 2 degree higher optimum temperature, 1.24 times higher activity, but the same pH optimum of pH 7.5 as mutant L200F. Both mutants L200F/D215K and L200F show good resistance to organic solvents and metal ions Corynebacterium pekinense
L200F/D215A site-directed mutagenesis Corynebacterium pekinense
L200F/D215E site-directed mutagenesis Corynebacterium pekinense
L200F/D215G site-directed mutagenesis Corynebacterium pekinense
L200F/D215K site-directed mutagenesis, compared to mutant L200F, the double mutant shows 2 dgree higher optimum temperature, 1.24 times higher activity, but the same pH optimum of pH 7.5 as mutant L200F. Both mutants L200F/D215K and L200F show good resistance to organic solvents and metal ions Corynebacterium pekinense

Organic Solvent Stability

Organic Solvent Comment Organism
additional information both mutants L200F/D215K and L200F show good resistance to organic solvents and metal ions Corynebacterium pekinense

Organism

Organism UniProt Comment Textmining
Corynebacterium pekinense
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-
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Corynebacterium pekinense AS1.299
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-
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Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
mutant L200F Corynebacterium pekinense
37
-
mutant L200F/D215K Corynebacterium pekinense

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
half-life of mutant L200F/D215K is 4.16 h, of mutant L200F 3.71 h Corynebacterium pekinense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
mutants L200F/D215K and L200F Corynebacterium pekinense