Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-threonine | - |
Glycine max | |
additional information | enzyme is not inhibited by other aspartate-derived amino acids than threonine | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
NADPH | pH 8.0, 25°C | Glycine max | |
0.034 | - |
NADP+ | pH 8.0, 25°C | Glycine max | |
0.039 | - |
NADPH | pH 8.0, 25°C | Glycine max | |
0.098 | - |
L-aspartate 4-semialdehyde | cosubstrate NADPH, pH 8.0, 25°C | Glycine max | |
0.158 | - |
NADH | pH 8.0, 25°C | Glycine max | |
0.19 | - |
NADH | pH 8.0, 25°C | Glycine max | |
0.213 | - |
NADH | pH 8.0, 25°C | Glycine max | |
0.235 | - |
NADP+ | pH 8.0, 25°C | Glycine max | |
0.245 | - |
NADP+ | pH 8.0, 25°C | Glycine max | |
0.275 | - |
L-homoserine | cosubstrate NADP+, pH 8.0, 25°C | Glycine max | |
0.569 | - |
L-aspartate 4-semialdehyde | cosubstrate NADH, pH 8.0, 25°C | Glycine max | |
0.69 | - |
L-homoserine | cosubstrate NADP+, pH 8.0, 25°C | Glycine max | |
0.845 | - |
L-aspartate 4-semialdehyde | cosubstrate NADH, pH 8.0, 25°C | Glycine max | |
1.08 | - |
L-homoserine | cosubstrate NADP+, pH 8.0, 25°C | Glycine max | |
1.19 | - |
L-aspartate 4-semialdehyde | cosubstrate NADH, pH 8.0, 25°C | Glycine max | |
1.25 | - |
L-aspartate 4-semialdehyde | cosubstrate NADPH, pH 8.0, 25°C | Glycine max | |
2.19 | - |
L-aspartate 4-semialdehyde | cosubstrate NADPH, pH 8.0, 25°C | Glycine max | |
2.7 | - |
NAD+ | pH 8.0, 25°C | Glycine max | |
9.57 | - |
L-homoserine | cosubstrate NAD+, pH 8.0, 25°C | Glycine max | |
13.4 | - |
L-homoserine | cosubstrate NAD+, pH 8.0, 25°C | Glycine max | |
17.4 | - |
L-homoserine | cosubstrate NAD+, pH 8.0, 25°C | Glycine max | |
24.1 | - |
NAD+ | pH 8.0, 25°C | Glycine max | |
24.9 | - |
NAD+ | pH 8.0, 25°C | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40600, calculated, 2 * 40000, SDS-PAGE | Glycine max |
40600 | - |
2 * 40600, calculated, 2 * 40000, SDS-PAGE | Glycine max |
70000 | - |
gel filtration | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | O63067 | bifunctional aspartokinase-homoserine dehydrogenase AK-HSD-2 | - |
Glycine max | O65027 | bifunctional aspartokinase-homoserine dehydrogenase AK-HSD-1 | - |
Glycine max | Q3S3F6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Glycine max |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H + H+ | ordered bi bi kinetic mechanism in which nicotinamide cofactor binds first and leaves last in the reaction sequence | Glycine max |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13 | - |
pH 8.0, 25°C | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + NADH | - |
Glycine max | L-homoserine + NAD+ | - |
r | |
L-aspartate 4-semialdehyde + NADPH | - |
Glycine max | L-homoserine + NADP+ | - |
r | |
L-homoserine + NAD+ | - |
Glycine max | L-aspartate 4-semialdehyde + NADH | - |
r | |
L-homoserine + NADP+ | - |
Glycine max | L-aspartate 4-semialdehyde + NADPH | - |
r | |
additional information | enzyme does not show aspartate kinase activity | Glycine max | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40600, calculated, 2 * 40000, SDS-PAGE | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
AK-HSD-1 | - |
Glycine max |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
L-homoserine | cosubstrate NADP+, pH 8.0, 25°C | Glycine max | |
0.052 | - |
L-homoserine | cosubstrate NADP+, pH 8.0, 25°C | Glycine max | |
0.07 | - |
L-homoserine | cosubstrate NADP+, pH 8.0, 25°C | Glycine max | |
1.43 | - |
L-homoserine | cosubstrate NAD+, pH 8.0, 25°C | Glycine max | |
2.8 | - |
L-aspartate 4-semialdehyde | cosubstrate NADPH, pH 8.0, 25°C | Glycine max | |
7 | - |
L-homoserine | cosubstrate NAD+, pH 8.0, 25°C | Glycine max | |
9.68 | - |
L-aspartate 4-semialdehyde | cosubstrate NADH, pH 8.0, 25°C | Glycine max | |
10.15 | - |
L-homoserine | cosubstrate NAD+, pH 8.0, 25°C | Glycine max | |
11.58 | - |
L-aspartate 4-semialdehyde | cosubstrate NADPH, pH 8.0, 25°C | Glycine max | |
12.98 | - |
L-aspartate 4-semialdehyde | cosubstrate NADPH, pH 8.0, 25°C | Glycine max | |
19.3 | - |
L-aspartate 4-semialdehyde | cosubstrate NADH, pH 8.0, 25°C | Glycine max | |
22.58 | - |
L-aspartate 4-semialdehyde | cosubstrate NADH, pH 8.0, 25°C | Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | GmHSD displays a 1.6fold preference for NADPH over NADH as the cofactor in the oxidation reaction. In the reduction reaction NADP+ is favored nearly 4fold as the cofactor | Glycine max | |
NADPH | GmHSD displays a 1.6fold preference for NADPH over NADH as the cofactor in the oxidation reaction. In the reduction reaction NADP+ is favored nearly 4fold as the cofactor | Glycine max |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
160 | 240 | L-threonine | pH 8.0, 25°C | Glycine max |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.039 | - |
L-homoserine | cosubstrate NADP+, pH 8.0, 25°C | Glycine max | |
0.082 | - |
L-homoserine | cosubstrate NAD+, pH 8.0, 25°C | Glycine max | |
0.095 | - |
L-homoserine | cosubstrate NADP+, pH 8.0, 25°C | Glycine max | |
0.18 | - |
NADP+ | pH 8.0, 25°C | Glycine max | |
0.256 | - |
L-homoserine | cosubstrate NADP+, pH 8.0, 25°C | Glycine max | |
0.268 | - |
NADP+ | pH 8.0, 25°C | Glycine max | |
0.281 | - |
NAD+ | pH 8.0, 25°C | Glycine max | |
0.421 | - |
NAD+ | pH 8.0, 25°C | Glycine max | |
0.53 | - |
NAD+ | pH 8.0, 25°C | Glycine max | |
0.731 | - |
L-homoserine | cosubstrate NAD+, pH 8.0, 25°C | Glycine max | |
0.757 | - |
L-homoserine | cosubstrate NAD+, pH 8.0, 25°C | Glycine max | |
2.07 | - |
NADP+ | pH 8.0, 25°C | Glycine max | |
5.29 | - |
L-aspartate 4-semialdehyde | cosubstrate NADPH, pH 8.0, 25°C | Glycine max | |
10.39 | - |
L-aspartate 4-semialdehyde | cosubstrate NADPH, pH 8.0, 25°C | Glycine max | |
16.22 | - |
L-aspartate 4-semialdehyde | cosubstrate NADH, pH 8.0, 25°C | Glycine max | |
17.02 | - |
L-aspartate 4-semialdehyde | cosubstrate NADH, pH 8.0, 25°C | Glycine max | |
26.73 | - |
L-aspartate 4-semialdehyde | cosubstrate NADH, pH 8.0, 25°C | Glycine max | |
28.54 | - |
L-aspartate 4-semialdehyde | cosubstrate NADPH, pH 8.0, 25°C | Glycine max | |
61.29 | - |
NADH | pH 8.0, 25°C | Glycine max | |
90.61 | - |
NADH | pH 8.0, 25°C | Glycine max | |
101.4 | - |
NADPH | pH 8.0, 25°C | Glycine max | |
118.9 | - |
NADH | pH 8.0, 25°C | Glycine max | |
296.2 | - |
NADPH | pH 8.0, 25°C | Glycine max | |
331.2 | - |
NADPH | pH 8.0, 25°C | Glycine max |