Literature summary for 1.1.1.3 extracted from

Yilmaz, E.I.; Caydasi, A.K.; Ozcengiz, G.
Targeted disruption of homoserine dehydrogenase gene and its effect on cephamycin C production in Streptomyces clavuligerus (2008), J. Ind. Microbiol. Biotechnol., 35, 1-7.

Search for more literature for 1.1.1.3

Cloned(Commentary)

Cloned (Comment)	Organism
gene hom, DNA and amino acid sequence determination and analysis, expression in the auxotroph mutant Escherichia coli CGSC 5075	Streptomyces clavuligerus

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a hom disruption mutant by insertional inactivation via double crossover leading to up to 4.3fold and 2fold increases in intracellular free L-lysine concentration and specific cephamycin C production, respectively, during stationary phase in chemically defined medium, overview	Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-homoserine + NAD(P)+	Streptomyces clavuligerus	-	L-aspartate 4-semialdehyde + NAD(P)H	-	r
L-homoserine + NAD(P)+	Streptomyces clavuligerus NRRL 3585	-	L-aspartate 4-semialdehyde + NAD(P)H	-	r

Organism

Organism	UniProt	Comment	Textmining
Streptomyces clavuligerus	Q56R01	gene hom	-
Streptomyces clavuligerus NRRL 3585	Q56R01	gene hom	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-homoserine + NAD(P)+	-	Streptomyces clavuligerus	L-aspartate 4-semialdehyde + NAD(P)H	-	r
L-homoserine + NAD(P)+	-	Streptomyces clavuligerus NRRL 3585	L-aspartate 4-semialdehyde + NAD(P)H	-	r

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Streptomyces clavuligerus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Streptomyces clavuligerus

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Streptomyces clavuligerus
NADPH	-	Streptomyces clavuligerus

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Release 2023.1 (January 2023)