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Literature summary for 1.1.1.3 extracted from

  • Rognes, S.E.; Dewaele, E.; Aas, S.F.; Jacobs, M.; Frankard, V.
    Transcriptional and biochemical regulation of a novel Arabidopsis thaliana bifunctional aspartate kinase-homoserine dehydrogenase gene isolated by functional complementation of a yeast hom6 mutant (2003), Plant Mol. Biol., 51, 281-294.
    View publication on PubMed
Search for more literature for 1.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
gene akthr2, DNA sequence determination and analysis, located on chromosome 4, subcloning in Escherichia coli strain DH5alpha, functional complementation of the Saccharomyces cerevisiae homoserine dehydrogenase-deficient hom6 mutant strain and of the aspartate kinase-deficient hom3 mutant strain, conferring L-threonine and L-methionine prototrophy to the yeast cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of transgenic Arabidopsis thaliana plants by transformation with gene akthr2 via Agrobacterium tumefaciens infection, determination of expression patterns of the gene akthr1 ans akthr2 in the transgenic plants Arabidopsis thaliana
additional information mutant strain M20-20D is deficient in gene HOM6 and shows no activity, the defect can be complemented by recombinant expression of the Arabidopsis thaliana gene akthr2 in the mutant yeast cells Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
L-threonine
-
 Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for aspartate kinase activity Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-homoserine + NADP+ Saccharomyces cerevisiae
-
 L-aspartate 4-semialdehyde + NADPH
-
 ?
L-homoserine + NADP+ Arabidopsis thaliana
-
 L-aspartate 4-semialdehyde + NADPH
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 var. Bensheim showing threonine and methionine prototrophy, bifunctional enzyme showing aspartate dehydrogenase and aspartate kinase activity, gene akthr2
-
Saccharomyces cerevisiae
-
 mutant strain M20-20D and wild-type strain S2207A
-

Reaction

Reaction Comment Organism Reaction ID
L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H + H+ bifunctional enzyme showing aspartate kinase, 2.7.2.4, and homoserine dehydrogenase activities Arabidopsis thaliana
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-
meristem
-
 Arabidopsis thaliana
-
additional information akthr2 is not or time-restricted expressed in stem, gynoecium, and during seed formation Arabidopsis thaliana
-
stamen
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-homoserine + NADP+
-
 Saccharomyces cerevisiae L-aspartate 4-semialdehyde + NADPH
-
 ?
L-homoserine + NADP+
-
 Arabidopsis thaliana L-aspartate 4-semialdehyde + NADPH
-
 ?
L-homoserine + NADP+
-
 Arabidopsis thaliana L-aspartate 4-semialdehyde + NADPH
-
 r

Synonyms

Synonyms Comment Organism
AK-HSDH
-
 Arabidopsis thaliana
aspartate kinase-homoserine dehydrogenase
-
 Arabidopsis thaliana
HSDH
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Saccharomyces cerevisiae
NADP+
-
 Arabidopsis thaliana
NADPH
-
 Arabidopsis thaliana