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Literature summary for 1.1.1.298 extracted from

  • Liang, B.; Sun, G.; Wang, Z.; Xiao, J.; Yang, J.
    Production of 3-hydroxypropionate using a novel malonyl-CoA-mediated biosynthetic pathway in genetically engineered E. coli strain (2019), Green Chem., 21, 6103-6115 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene mcr, recombinant expression of MCR enzyme mutant in Escherichia coli strain CE43(DE3), coexpression with malonyl-CoA synthetase (MatB) from Rhodopseudomonas palustris, native transhydrogenase (PntAB), and NAD kinase (YfjB) Chloroflexus aurantiacus

Protein Variants

Protein Variants Comment Organism
additional information production of 3-hydroxypropionate using a novel malonyl-CoA-mediated biosynthetic pathway in genetically engineered Escherichia coli strain. Heterologously coexpressing the mutant of malonyl-CoA reductase (MCR) from Chloroflexus aurantiacus and malonyl-CoA synthetase (MatB) from Rhodopseudomonas palustris in the Escherichia coli C43 (DE3) strain. To further enhance the production of 3-HP, native transhydrogenase (PntAB) and NAD kinase (YfjB) genes are expressed to increase the NADPH supply in Escherichia coli. The final genetically modified strain SGN78 shows a significant improvement in malonate utilization and produced 1.20 g/l of 3-HP in the flask culture. Identification of suitable malonate transporters in Rhodobacter capsulatus and Sinorhizobium meliloti, and coexpression of transporter MatB in Escherichia coli. The enzyme activity increases when the N-terminal and C-terminal regions of MCR are separated by fusing a flexible linker (GGGGS) between the two enzymatic units. Optimization of fermentation conditions and improvement of NADPH supply increase 3-HP production rate Chloroflexus aurantiacus
N940V/K1106W/S1114R site-directed mutagenesis, mutant N940V/K1106W/S1114R improves the catalytic efficiency by 14.2fold over the wild-type Chloroflexus aurantiacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonate semialdehyde + NADPH + H+ Chloroflexus aurantiacus
-
3-hydroxypropanoate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Chloroflexus aurantiacus Q6QQP7 bifunctional enzyme
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonate semialdehyde + NADPH + H+
-
Chloroflexus aurantiacus 3-hydroxypropanoate + NADP+
-
?

Subunits

Subunits Comment Organism
homodimer
-
Chloroflexus aurantiacus

Synonyms

Synonyms Comment Organism
malonyl-CoA reductase UniProt Chloroflexus aurantiacus
MCR
-
Chloroflexus aurantiacus
More see also EC 1.2.1.75 Chloroflexus aurantiacus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Chloroflexus aurantiacus