Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.298 extracted from

  • Strauss, G.; Fuchs, G.
    Enzymes of a novel autotrophic carbon dioxide fixation pathway in the phototrophic bacterium Chloroflexus aurantiacus, the 3-hydroxypropionate cycle (1993), Eur. J. Biochem., 215, 633-643.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxypropanoate + NADP+ Chloroflexus aurantiacus
-
malonate-semialdehyde + NADPH + H+
-
r
malonate-semialdehyde + NADPH + H+ Chloroflexus aurantiacus
-
3-hydroxypropionate + NADP+
-
r
additional information Chloroflexus aurantiacus enzyme is part of an autotrophic CO2 fixation pathway in which acetyl-CoA is carboxylated and reductively converted via 3-hydroxypropionate to propionyl-CoA. Propionyl-CoA is carboxylated and converted via succinyl-CoA and CoA transfer to malyl-CoA. Malyl-CoA is cleaved to acetyl-CoA and glyoxylate. Thereby, the first CO, acceptor molecule acetyl-CoA is regenerated, completing the cycle and the net CO, fixation product glyoxylate is released ?
-
?

Organism

Organism UniProt Comment Textmining
Chloroflexus aurantiacus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
substrate 3-hydroxypropionate, pH 9.0, 45°C Chloroflexus aurantiacus
0.04
-
substrate malonate-semialdehyde, pH 9.0, 45°C Chloroflexus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypropanoate + NADP+
-
Chloroflexus aurantiacus malonate-semialdehyde + NADPH + H+
-
r
malonate-semialdehyde + NADPH + H+
-
Chloroflexus aurantiacus 3-hydroxypropionate + NADP+
-
r
additional information enzyme is part of an autotrophic CO2 fixation pathway in which acetyl-CoA is carboxylated and reductively converted via 3-hydroxypropionate to propionyl-CoA. Propionyl-CoA is carboxylated and converted via succinyl-CoA and CoA transfer to malyl-CoA. Malyl-CoA is cleaved to acetyl-CoA and glyoxylate. Thereby, the first CO, acceptor molecule acetyl-CoA is regenerated, completing the cycle and the net CO, fixation product glyoxylate is released Chloroflexus aurantiacus ?
-
?

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NAD+ Chloroflexus aurantiacus
NADP+
-
Chloroflexus aurantiacus
NADPH
-
Chloroflexus aurantiacus