BRENDA - Enzyme Database show
show all sequences of 1.1.1.295

Investigating inducible short-chain alcohol dehydrogenases/reductases clarifies rice oryzalexin biosynthesis

Kitaoka, N.; Wu, Y.; Zi, J.; Peters, R.J.; Plant J. 88, 271-279 (2016)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
oryzalexin A
pH 8.0, 30°C; pH 8.0, 30°C
Oryza sativa
0.2
-
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide
pH 8.0, 30°C
Oryza sativa
0.9
-
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide
pH 8.0, 30°C
Oryza sativa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryza sativa
Q7FAE1
-
-
Oryza sativa
Q7FAE2
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2alpha-hydroxy-ent-cassadiene + NAD+
-
741178
Oryza sativa
? + NADH + H+
-
-
-
?
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide + NAD+
-
741178
Oryza sativa
momilactone A + NADH + H+
-
-
-
?
oryzalexin D + NAD+
-
741178
Oryza sativa
oryzalexin A + NADH + H+
oxidation of the 7beta-hydroxyl group of oryzalexin D
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.02
-
oryzalexin A
pH 8.0, 30°C
Oryza sativa
0.023
-
oryzalexin A
pH 8.0, 30°C
Oryza sativa
0.08
-
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide
pH 8.0, 30°C
Oryza sativa
0.4
-
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide
pH 8.0, 30°C
Oryza sativa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
oryzalexin A
pH 8.0, 30°C
Oryza sativa
0.2
-
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide
pH 8.0, 30°C
Oryza sativa
0.9
-
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide
pH 8.0, 30°C
Oryza sativa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2alpha-hydroxy-ent-cassadiene + NAD+
-
741178
Oryza sativa
? + NADH + H+
-
-
-
?
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide + NAD+
-
741178
Oryza sativa
momilactone A + NADH + H+
-
-
-
?
oryzalexin D + NAD+
-
741178
Oryza sativa
oryzalexin A + NADH + H+
oxidation of the 7beta-hydroxyl group of oryzalexin D
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.02
-
oryzalexin A
pH 8.0, 30°C
Oryza sativa
0.023
-
oryzalexin A
pH 8.0, 30°C
Oryza sativa
0.08
-
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide
pH 8.0, 30°C
Oryza sativa
0.4
-
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide
pH 8.0, 30°C
Oryza sativa
General Information
General Information
Commentary
Organism
physiological function
isoform MS2 more efficiently catalyzes the final oxidation to produce momilactone A than isoform MS1
Oryza sativa
General Information (protein specific)
General Information
Commentary
Organism
physiological function
isoform MS2 more efficiently catalyzes the final oxidation to produce momilactone A than isoform MS1
Oryza sativa
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.09
-
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide
pH 8.0, 30°C
Oryza sativa
0.3
-
oryzalexin A
pH 8.0, 30°C
Oryza sativa
0.4
-
oryzalexin A
pH 8.0, 30°C
Oryza sativa
2
-
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide
pH 8.0, 30°C
Oryza sativa
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.09
-
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide
pH 8.0, 30°C
Oryza sativa
0.3
-
oryzalexin A
pH 8.0, 30°C
Oryza sativa
0.4
-
oryzalexin A
pH 8.0, 30°C
Oryza sativa
2
-
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide
pH 8.0, 30°C
Oryza sativa
Other publictions for EC 1.1.1.295
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741178
Kitaoka
Investigating inducible short- ...
Oryza sativa
Plant J.
88
271-279
2016
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3
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2
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6
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4
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4
-
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-
-
6
-
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-
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4
-
-
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-
1
1
-
4
4
680848
Shimura
Identification of a biosynthet ...
Oryza sativa
J. Biol. Chem.
282
34013-34018
2007
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-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
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-
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1
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1
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-
-
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678769
Atawong
Biosynthesis of rice phytoalex ...
Oryza sativa
Biosci. Biotechnol. Biochem.
66
566-570
2002
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-
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1
1
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1
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1
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2
-
1
1
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-
1
1
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2
-
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-
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-
2
-
-
-
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-
1
1
-
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-
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-
1
-
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2
-
1
1
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-
1
1
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