BRENDA - Enzyme Database show
show all sequences of 1.1.1.295

Biosynthesis of rice phytoalexin: enzymatic conversion of 3beta-hydroxy-9beta-pimara-7,15-dien-19,6beta-olide to momilactone A

Atawong, A.; Hasegawa, M.; Kodama, O.; Biosci. Biotechnol. Biochem. 66, 566-570 (2002)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.036
-
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide
cofactor: NAD+
Oryza sativa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Oryza sativa
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryza sativa
-
L. cv. Nipponbare
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
husk
-
Oryza sativa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide + NAD+
-
678769
Oryza sativa
momilactone A + NADH
-
-
-
?
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide + NADP+
reaction with NADP+ is 70% of the activity with NAD+
678769
Oryza sativa
momilactone A + NADPH
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
48
-
-
Oryza sativa
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
36
72
36°C: about 60% of maximal activity, 72°C: about 60% of maximal activity
Oryza sativa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Oryza sativa
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10
pH 6.0: about 50% of maximal activity, pH 10.0: about 60% of maximal activity
Oryza sativa
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Oryza sativa
NADP+
70% of the activity with NAD+
Oryza sativa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Oryza sativa
NADP+
70% of the activity with NAD+
Oryza sativa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.036
-
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide
cofactor: NAD+
Oryza sativa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Oryza sativa
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
husk
-
Oryza sativa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide + NAD+
-
678769
Oryza sativa
momilactone A + NADH
-
-
-
?
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide + NADP+
reaction with NADP+ is 70% of the activity with NAD+
678769
Oryza sativa
momilactone A + NADPH
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
48
-
-
Oryza sativa
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
36
72
36°C: about 60% of maximal activity, 72°C: about 60% of maximal activity
Oryza sativa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Oryza sativa
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10
pH 6.0: about 50% of maximal activity, pH 10.0: about 60% of maximal activity
Oryza sativa
Other publictions for EC 1.1.1.295
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741178
Kitaoka
Investigating inducible short- ...
Oryza sativa
Plant J.
88
271-279
2016
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
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6
-
-
-
-
4
-
-
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-
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-
-
-
-
-
-
-
-
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-
4
-
-
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-
-
-
-
-
-
-
-
6
-
-
-
-
4
-
-
-
-
-
1
1
-
4
4
680848
Shimura
Identification of a biosynthet ...
Oryza sativa
J. Biol. Chem.
282
34013-34018
2007
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
678769
Atawong
Biosynthesis of rice phytoalex ...
Oryza sativa
Biosci. Biotechnol. Biochem.
66
566-570
2002
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
1
1
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
2
-
1
1
-
-
1
1
-
-
-
-
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