BRENDA - Enzyme Database show
show all sequences of 1.1.1.294

Participation of chlorophyll b reductase in the initial step of the degradation of light-harvesting chlorophyll a/b-protein complexes in Arabidopsis

Horie, Y.; Ito, H.; Kusaba, M.; Tanaka, R.; Tanaka, A.; J. Biol. Chem. 284, 17449-17456 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
coding region of chlorophyll b reductase (NOL) lacking its transit peptide amplified and cloned into pET-30a(+) at NspV and HindIII sites, expressed in Escherichia coli Rosetta DE3
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
additional information
Arabidopsis nol/nyc1 double mutant, when the genes for chlorophyll b reductases NOL and NYC1 are disrupted, chlorophyll b and photosystem II are not degraded during senescence, whereas other pigment complexes completely disappear
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
chlorophyll b reductase decreases during senescence and finally disappears after 8-day-dark incubation
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Purification (Commentary)
Commentary
Organism
by centrifugation, sonication, on a nickel column and by ultrafiltration
Arabidopsis thaliana
Storage Stability
Storage Stability
Organism
4C, 24 hours
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
chlorophyll b + NADPH
when purified trimeric photosystem II is incubated with recombinant chlorophyll b reductase (NOL), conversion of chlorophyll b in photosystem II to 7-hydroxymethyl chlorophyll a. Chlorophyll b reductase catalyzes the initial step of photosystem II degradation
698907
Arabidopsis thaliana
7-hydroxymethyl chlorophyll a + NADP+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
coding region of chlorophyll b reductase (NOL) lacking its transit peptide amplified and cloned into pET-30a(+) at NspV and HindIII sites, expressed in Escherichia coli Rosetta DE3
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
Arabidopsis nol/nyc1 double mutant, when the genes for chlorophyll b reductases NOL and NYC1 are disrupted, chlorophyll b and photosystem II are not degraded during senescence, whereas other pigment complexes completely disappear
Arabidopsis thaliana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
chlorophyll b reductase decreases during senescence and finally disappears after 8-day-dark incubation
Arabidopsis thaliana
Purification (Commentary) (protein specific)
Commentary
Organism
by centrifugation, sonication, on a nickel column and by ultrafiltration
Arabidopsis thaliana
Storage Stability (protein specific)
Storage Stability
Organism
4C, 24 hours
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
chlorophyll b + NADPH
when purified trimeric photosystem II is incubated with recombinant chlorophyll b reductase (NOL), conversion of chlorophyll b in photosystem II to 7-hydroxymethyl chlorophyll a. Chlorophyll b reductase catalyzes the initial step of photosystem II degradation
698907
Arabidopsis thaliana
7-hydroxymethyl chlorophyll a + NADP+
-
-
-
?
Other publictions for EC 1.1.1.294
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740819
Jibran
Staying green postharvest: how ...
Arabidopsis thaliana
J. Exp. Bot.
66
6849-6862
2015
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741106
Sato
Chlorophyll b degradation by c ...
Arabidopsis thaliana
Photosyn. Res.
126
249-259
2015
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741177
Jia
Accumulation of the NON-YELLOW ...
Arabidopsis thaliana
Plant J.
81
586-596
2015
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737240
Peng
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Chlorophyll a/b binding protei ...
Citrus reticulata, Citrus reticulata Blanco
Sci. Hortic.
160
37-43
2013
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726228
Nakajima
Chlorophyll b reductase plays ...
Arabidopsis thaliana
Plant Physiol.
160
261-273
2012
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714334
Tanaka
Chlorophyll cycle regulates th ...
Arabidopsis thaliana, Hordeum vulgare, Oryza sativa
Biochim. Biophys. Acta
1807
968-976
2011
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698907
Horie
Participation of chlorophyll b ...
Arabidopsis thaliana
J. Biol. Chem.
284
17449-17456
2009
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700752
Sato
Two short-chain dehydrogenase/ ...
Oryza sativa
Plant J.
57
120-131
2009
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700839
Barry
The stay-green revolution: Rec ...
Arabidopsis thaliana, Oryza sativa
Plant Sci.
176
325-333
2009
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689462
Kusaba
Rice NON-YELLOW COLORING1 is i ...
Oryza sativa
Plant Cell
19
1362-1375
2007
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676326
Ruediger
Biosynthesis of chlorophyll b ...
Hordeum vulgare
Photosyn. Res.
74
187-193
2002
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80748
Scheumann
Chlorophyll b reduction during ...
Hordeum vulgare
Planta
209
364-370
1999
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639802
Vezitskii
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Activity of chlorophyll synthe ...
Helianthus annuus
Russ. J. Plant Physiol.
46
502-506
1999
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673428
Scheumann
Substrate specificity of chlor ...
Hordeum vulgare
Eur. J. Biochem.
242
163-170
1996
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