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show all sequences of 1.1.1.292

Mouse AKR1E1 is an ortholog of pig liver NADPH dependent 1,5-anhydro-D-fructose reductase

Sakuma, M.; Kubota, S.; Biosci. Biotechnol. Biochem. 72, 872-876 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
AKR1E1 gene, cloning from liver RNA, DNA and amino acid sequence determination, analysis, and comparison, expression in Escherichia coli strain BL21 (DE3)
Mus musculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.02
-
1,5-Anhydro-D-fructose
pH 7.0, 37°C, recombinant enzyme
Mus musculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1,5-anhydro-D-mannitol + NADP+
Mus musculus
-
1,5-anhydro-D-fructose + NADPH
-
-
r
1,5-anhydro-D-mannitol + NADP+
Mus musculus C57/BL6J
-
1,5-anhydro-D-fructose + NADPH
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
Q9DCT1
C57BL/6J mice
-
Mus musculus C57/BL6J
Q9DCT1
C57BL/6J mice
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme 17.6fold from Escherichia coli strain BL21 (DE3) by anion exchange and adsorption chromatography
Mus musculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,5-anhydro-D-mannitol + NADP+
-
685692
Mus musculus
1,5-anhydro-D-fructose + NADPH
-
-
-
r
1,5-anhydro-D-mannitol + NADP+
1,5-anhydro-D-fructose is the preferred substrate for the reductase reaction
685692
Mus musculus
1,5-anhydro-D-fructose + NADPH
-
-
-
r
1,5-anhydro-D-mannitol + NADP+
-
685692
Mus musculus C57/BL6J
1,5-anhydro-D-fructose + NADPH
-
-
-
r
1,5-anhydro-D-mannitol + NADP+
1,5-anhydro-D-fructose is the preferred substrate for the reductase reaction
685692
Mus musculus C57/BL6J
1,5-anhydro-D-fructose + NADPH
-
-
-
r
9,10-phenanthrenequinone + NADPH
-
685692
Mus musculus
? + NADP+
-
-
-
?
9,10-phenanthrenequinone + NADPH
-
685692
Mus musculus C57/BL6J
? + NADP+
-
-
-
?
butane-2,3-dione + NADPH
-
685692
Mus musculus
acetoin + NADP+
-
-
-
?
butane-2,3-dione + NADPH
-
685692
Mus musculus C57/BL6J
acetoin + NADP+
-
-
-
?
additional information
D-glucose, D-fructose, glucuronic acid, DL-glyceraldehyde, formaldehyde, and acetone are poor substrates, substrate specificity of the recombinant enzyme, overview
685692
Mus musculus
?
-
-
-
-
additional information
D-glucose, D-fructose, glucuronic acid, DL-glyceraldehyde, formaldehyde, and acetone are poor substrates, substrate specificity of the recombinant enzyme, overview
685692
Mus musculus C57/BL6J
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Mus musculus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
9.3
-
1,5-Anhydro-D-fructose
pH 7.0, 37°C, recombinant enzyme
Mus musculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at, reverse reaction
Mus musculus
Cloned(Commentary) (protein specific)
Commentary
Organism
AKR1E1 gene, cloning from liver RNA, DNA and amino acid sequence determination, analysis, and comparison, expression in Escherichia coli strain BL21 (DE3)
Mus musculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.02
-
1,5-Anhydro-D-fructose
pH 7.0, 37°C, recombinant enzyme
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1,5-anhydro-D-mannitol + NADP+
Mus musculus
-
1,5-anhydro-D-fructose + NADPH
-
-
r
1,5-anhydro-D-mannitol + NADP+
Mus musculus C57/BL6J
-
1,5-anhydro-D-fructose + NADPH
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme 17.6fold from Escherichia coli strain BL21 (DE3) by anion exchange and adsorption chromatography
Mus musculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,5-anhydro-D-mannitol + NADP+
-
685692
Mus musculus
1,5-anhydro-D-fructose + NADPH
-
-
-
r
1,5-anhydro-D-mannitol + NADP+
1,5-anhydro-D-fructose is the preferred substrate for the reductase reaction
685692
Mus musculus
1,5-anhydro-D-fructose + NADPH
-
-
-
r
1,5-anhydro-D-mannitol + NADP+
-
685692
Mus musculus C57/BL6J
1,5-anhydro-D-fructose + NADPH
-
-
-
r
1,5-anhydro-D-mannitol + NADP+
1,5-anhydro-D-fructose is the preferred substrate for the reductase reaction
685692
Mus musculus C57/BL6J
1,5-anhydro-D-fructose + NADPH
-
-
-
r
9,10-phenanthrenequinone + NADPH
-
685692
Mus musculus
? + NADP+
-
-
-
?
9,10-phenanthrenequinone + NADPH
-
685692
Mus musculus C57/BL6J
? + NADP+
-
-
-
?
butane-2,3-dione + NADPH
-
685692
Mus musculus
acetoin + NADP+
-
-
-
?
butane-2,3-dione + NADPH
-
685692
Mus musculus C57/BL6J
acetoin + NADP+
-
-
-
?
additional information
D-glucose, D-fructose, glucuronic acid, DL-glyceraldehyde, formaldehyde, and acetone are poor substrates, substrate specificity of the recombinant enzyme, overview
685692
Mus musculus
?
-
-
-
-
additional information
D-glucose, D-fructose, glucuronic acid, DL-glyceraldehyde, formaldehyde, and acetone are poor substrates, substrate specificity of the recombinant enzyme, overview
685692
Mus musculus C57/BL6J
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Mus musculus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
9.3
-
1,5-Anhydro-D-fructose
pH 7.0, 37°C, recombinant enzyme
Mus musculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at, reverse reaction
Mus musculus
Other publictions for EC 1.1.1.292
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739808
Schu
The structure of substrate-fre ...
Sinorhizobium meliloti
Acta Crystallogr. Sect. F
69
844-849
2013
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1
1
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1
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1
-
1
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685692
Sakuma
Mouse AKR1E1 is an ortholog of ...
Mus musculus, Mus musculus C57/BL6J
Biosci. Biotechnol. Biochem.
72
872-876
2008
-
-
1
-
-
-
-
1
-
-
-
2
-
2
-
-
1
-
-
-
-
-
10
-
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
-
-
-
10
-
1
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1
1
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-
-
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-
-
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-
667264
Kuehn
Catabolism of 1,5-anhydro-D-fr ...
Ensifer adhaerens, Ensifer adhaerens S-30.7.5
Appl. Environ. Microbiol.
72
1248-1257
2006
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-
1
-
-
-
-
3
-
-
3
-
-
2
-
-
1
-
-
-
2
1
11
1
-
-
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3
1
1
-
2
-
1
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-
1
2
-
-
-
-
-
-
3
-
-
3
-
-
-
-
1
-
-
2
1
11
1
-
-
-
3
1
1
-
1
-
-
-
-
-
-
667698
Dambe
Crystal structure of NADP(H)-d ...
Ensifer adhaerens
Biochemistry
45
10030-10042
2006
-
-
1
1
9
-
-
21
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
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13
-
-
-
2
-
-
-
-
-
1
2
1
9
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-
21
-
-
-
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-
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-
2
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13
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