BRENDA - Enzyme Database
show all sequences of 1.1.1.292

Catabolism of 1,5-anhydro-D-fructose in Sinorhizobium morelense S-30.7.5: discovery, characterization, and overexpression of a new 1,5-anhydro-D-fructose reductase and its application in sugar analysis and rare sugar synthesis

Kuehn, A.; Yu, S.; Giffhorn, F.; Appl. Environ. Microbiol. 72, 1248-1257 (2006)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
overexpression in Escherichia coli
Ensifer adhaerens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
NADPH
pH 6.5, 30C
Ensifer adhaerens
8.4
-
1,5-Anhydro-D-fructose
pH 6.5, 30C
Ensifer adhaerens
11
-
D-glucosone
pH 6.5, 30C
Ensifer adhaerens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35100
-
1 * 35100, MALDI-TOF-MS
Ensifer adhaerens
38200
-
gel filtration
Ensifer adhaerens
40000
-
1 * 40000, SDS-PAGE
Ensifer adhaerens
Organism
Organism
UniProt
Commentary
Textmining
Ensifer adhaerens
Q2I8V6
i.e. Ensifer adhaerens
-
Ensifer adhaerens S-30.7.5
Q2I8V6
i.e. Ensifer adhaerens
-
Purification (Commentary)
Purification (Commentary)
Organism
native and recombinant enzyme
Ensifer adhaerens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
288.9
-
native enzyme
Ensifer adhaerens
484
-
recombinant enzyme expressed in Escherichia coli
Ensifer adhaerens
Storage Stability
Storage Stability
Organism
-20C or 0C, 50 days, 50% loss of activity
Ensifer adhaerens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1,5-anhydro-D-fructose + NADPH + H+
-
667264
Ensifer adhaerens
1,5-anhydro-D-mannitol + NADP+
-
-
-
r
1,5-anhydro-D-fructose + NADPH + H+
-
667264
Ensifer adhaerens S-30.7.5
1,5-anhydro-D-mannitol + NADP+
-
-
-
r
1,5-anhydro-D-glucitol + NADP+
23% of the activity with 1,5-anhydro-D-mannitol
667264
Ensifer adhaerens
?
-
-
-
?
1,5-anhydro-D-glucitol + NADP+
23% of the activity with 1,5-anhydro-D-mannitol
667264
Ensifer adhaerens S-30.7.5
?
-
-
-
?
6-deoxy-D-glucosone + NADPH + H+
22% of the activity with 1,5-anhydro-D-fructose
667264
Ensifer adhaerens
6-deoxy-D-mannose + NADP+
-
-
-
?
6-deoxy-D-glucosone + NADPH + H+
22% of the activity with 1,5-anhydro-D-fructose
667264
Ensifer adhaerens S-30.7.5
6-deoxy-D-mannose + NADP+
-
-
-
?
D-allosone + NADPH + H+
10% of the activity with 1,5-anhydro-D-fructose
667264
Ensifer adhaerens
D-altrose + NADP+
-
-
-
?
D-allosone + NADPH + H+
10% of the activity with 1,5-anhydro-D-fructose
667264
Ensifer adhaerens S-30.7.5
D-altrose + NADP+
-
-
-
?
D-glucosone + NADPH + H+
22% of the activity with 1,5-anhydro-D-fructose
667264
Ensifer adhaerens
D-mannose + NADP+
-
-
-
?
D-glucosone + NADPH + H+
22% of the activity with 1,5-anhydro-D-fructose
667264
Ensifer adhaerens S-30.7.5
D-mannose + NADP+
-
-
-
?
D-xylosone + NADPH + H+
17% of the activity with 1,5-anhydro-D-fructose
667264
Ensifer adhaerens
D-lyxose + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 35100, MALDI-TOF-MS; 1 * 40000, SDS-PAGE
Ensifer adhaerens
Synonyms
Synonyms
Commentary
Organism
1,5-anhydro-D-fructose reductase (ambiguous)
-
Ensifer adhaerens
AFR
-
Ensifer adhaerens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
63.2
-
D-glucosone
pH 6.5, 30C
Ensifer adhaerens
286.7
-
1,5-Anhydro-D-fructose
pH 6.5, 30C
Ensifer adhaerens
1300
-
1,5-Anhydro-D-fructose
pH 6.5, 30C
Ensifer adhaerens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Ensifer adhaerens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.2
8.8
about 50% of maximal activity at pH 5.5 and at pH 8.8
Ensifer adhaerens
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Ensifer adhaerens
NADPH
inactive towards NADH
Ensifer adhaerens
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Ensifer adhaerens
isoelectric focusing
-
4.3
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Escherichia coli
Ensifer adhaerens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Ensifer adhaerens
NADPH
inactive towards NADH
Ensifer adhaerens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
NADPH
pH 6.5, 30C
Ensifer adhaerens
8.4
-
1,5-Anhydro-D-fructose
pH 6.5, 30C
Ensifer adhaerens
11
-
D-glucosone
pH 6.5, 30C
Ensifer adhaerens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35100
-
1 * 35100, MALDI-TOF-MS
Ensifer adhaerens
38200
-
gel filtration
Ensifer adhaerens
40000
-
1 * 40000, SDS-PAGE
Ensifer adhaerens
Purification (Commentary) (protein specific)
Commentary
Organism
native and recombinant enzyme
Ensifer adhaerens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
288.9
-
native enzyme
Ensifer adhaerens
484
-
recombinant enzyme expressed in Escherichia coli
Ensifer adhaerens
Storage Stability (protein specific)
Storage Stability
Organism
-20C or 0C, 50 days, 50% loss of activity
Ensifer adhaerens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1,5-anhydro-D-fructose + NADPH + H+
-
667264
Ensifer adhaerens
1,5-anhydro-D-mannitol + NADP+
-
-
-
r
1,5-anhydro-D-fructose + NADPH + H+
-
667264
Ensifer adhaerens S-30.7.5
1,5-anhydro-D-mannitol + NADP+
-
-
-
r
1,5-anhydro-D-glucitol + NADP+
23% of the activity with 1,5-anhydro-D-mannitol
667264
Ensifer adhaerens
?
-
-
-
?
1,5-anhydro-D-glucitol + NADP+
23% of the activity with 1,5-anhydro-D-mannitol
667264
Ensifer adhaerens S-30.7.5
?
-
-
-
?
6-deoxy-D-glucosone + NADPH + H+
22% of the activity with 1,5-anhydro-D-fructose
667264
Ensifer adhaerens
6-deoxy-D-mannose + NADP+
-
-
-
?
6-deoxy-D-glucosone + NADPH + H+
22% of the activity with 1,5-anhydro-D-fructose
667264
Ensifer adhaerens S-30.7.5
6-deoxy-D-mannose + NADP+
-
-
-
?
D-allosone + NADPH + H+
10% of the activity with 1,5-anhydro-D-fructose
667264
Ensifer adhaerens
D-altrose + NADP+
-
-
-
?
D-allosone + NADPH + H+
10% of the activity with 1,5-anhydro-D-fructose
667264
Ensifer adhaerens S-30.7.5
D-altrose + NADP+
-
-
-
?
D-glucosone + NADPH + H+
22% of the activity with 1,5-anhydro-D-fructose
667264
Ensifer adhaerens
D-mannose + NADP+
-
-
-
?
D-glucosone + NADPH + H+
22% of the activity with 1,5-anhydro-D-fructose
667264
Ensifer adhaerens S-30.7.5
D-mannose + NADP+
-
-
-
?
D-xylosone + NADPH + H+
17% of the activity with 1,5-anhydro-D-fructose
667264
Ensifer adhaerens
D-lyxose + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 35100, MALDI-TOF-MS; 1 * 40000, SDS-PAGE
Ensifer adhaerens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
63.2
-
D-glucosone
pH 6.5, 30C
Ensifer adhaerens
286.7
-
1,5-Anhydro-D-fructose
pH 6.5, 30C
Ensifer adhaerens
1300
-
1,5-Anhydro-D-fructose
pH 6.5, 30C
Ensifer adhaerens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Ensifer adhaerens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.2
8.8
about 50% of maximal activity at pH 5.5 and at pH 8.8
Ensifer adhaerens
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Ensifer adhaerens
isoelectric focusing
-
4.3
Other publictions for EC 1.1.1.292
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739808
Schu
The structure of substrate-fre ...
Sinorhizobium meliloti
Acta Crystallogr. Sect. F
69
844-849
2013
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685692
Sakuma
Mouse AKR1E1 is an ortholog of ...
Mus musculus, Mus musculus C57/BL6J
Biosci. Biotechnol. Biochem.
72
872-876
2008
-
-
1
-
-
-
-
1
-
-
-
2
-
2
-
-
1
-
-
-
-
-
10
-
4
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
-
-
-
10
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
667264
Kuehn
Catabolism of 1,5-anhydro-D-fr ...
Ensifer adhaerens, Ensifer adhaerens S-30.7.5
Appl. Environ. Microbiol.
72
1248-1257
2006
-
-
1
-
-
-
-
3
-
-
3
-
-
2
-
-
1
-
-
-
2
1
11
1
2
-
-
-
3
1
1
-
2
-
1
-
-
-
1
2
-
-
-
-
-
-
3
-
-
3
-
-
-
-
1
-
-
2
1
11
1
-
-
-
3
1
1
-
1
-
-
-
-
-
-
667698
Dambe
Crystal structure of NADP(H)-d ...
Ensifer adhaerens
Biochemistry
45
10030-10042
2006
-
-
1
1
9
-
-
21
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
13
-
-
-
2
-
-
-
-
-
1
2
1
9
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-