Cloned (Comment) | Organism |
---|---|
genes sldSLC and sboA, DNA and amino acid sequence determination and analysis, genetic organization, a putational transcription regulator encoded by gene sboR is located upstream, sboRA comprises an operon, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Gluconobacter frateurii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of disruption mutants, a mutant defective in sboA shows significantly reduced growth on L-sorbose, while a sboR mutant grows on L-sorbose even better than the wild-type strain with higher NADPH-SR activity in cytoplasm fractions | Gluconobacter frateurii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Gluconobacter frateurii | 5737 | - |
membrane | - |
Gluconobacter frateurii | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28320 | - |
3 * 29000, recombinant SboA, SDS-PAGE, 3 * 28320, SboA, sequence calculation | Gluconobacter frateurii |
29000 | - |
3 * 29000, recombinant SboA, SDS-PAGE, 3 * 28320, SboA, sequence calculation | Gluconobacter frateurii |
87000 | - |
recombinant SboA, gel filtration | Gluconobacter frateurii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucitol + NADP+ | Gluconobacter frateurii | - |
D-sorbose + NADPH + H+ | - |
r | |
D-glucitol + NADP+ | Gluconobacter frateurii | - |
L-sorbose + NADPH + H+ | - |
r | |
D-glucitol + NADP+ | Gluconobacter frateurii THD32 | - |
D-sorbose + NADPH + H+ | - |
r | |
D-glucitol + NADP+ | Gluconobacter frateurii THD32 | - |
L-sorbose + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter frateurii | - |
genes sldSLC and sboA | - |
Gluconobacter frateurii THD32 | - |
genes sldSLC and sboA | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | SboA is required for effective growth on D- or L-sorbose, the organism also grows on glycerol | Gluconobacter frateurii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucitol + NADP+ | - |
Gluconobacter frateurii | D-sorbose + NADPH + H+ | - |
r | |
D-glucitol + NADP+ | - |
Gluconobacter frateurii THD32 | D-sorbose + NADPH + H+ | - |
r | |
D-glucitol + NADP+ | - |
Gluconobacter frateurii | L-sorbose + NADPH + H+ | - |
r | |
D-glucitol + NADP+ | - |
Gluconobacter frateurii THD32 | L-sorbose + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 29000, recombinant SboA, SDS-PAGE, 3 * 28320, SboA, sequence calculation | Gluconobacter frateurii |
Synonyms | Comment | Organism |
---|---|---|
FAD-dependent D-sorbitol dehydrogenase | - |
Gluconobacter frateurii |
flavin adenine dinucleotide-dependent D-sorbitol dehydrogenase | - |
Gluconobacter frateurii |
NADPH-dependent L-sorbose reductase | - |
Gluconobacter frateurii |
NADPH-SR | - |
Gluconobacter frateurii |
SboA | - |
Gluconobacter frateurii |
SLDH | - |
Gluconobacter frateurii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | oxidation of L-sorbitol | Gluconobacter frateurii |
9 | - |
reduction reaction, assay at | Gluconobacter frateurii |
10 | - |
oxidation of D-sorbitol | Gluconobacter frateurii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dependent on | Gluconobacter frateurii | |
NADP+ | dependent on | Gluconobacter frateurii | |
NADPH | dependent on | Gluconobacter frateurii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Gluconobacter frateurii | SboA, sequence calculation | - |
5.72 |