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show all sequences of 1.1.1.287

Cloning, purification and characterization of an NAD-dependent D-arabitol dehydrogenase from acetic acid bacterium, Acetobacter suboxydans

Cheng, H.; Li, Z.; Jiang, N.; Deng, Z.; Protein J. 28, 263-272 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Gluconobacter oxydans
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
10 mM, complete inhibition. Up to 80% renaturation by 100 mM EDTA
Gluconobacter oxydans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.5
-
D-arabitol
pH 8.5, 25°C
Gluconobacter oxydans
18.5
-
xylitol
pH 8.5, 25°C
Gluconobacter oxydans
78.5
-
D-mannitol
pH 8.5, 25°C
Gluconobacter oxydans
127.3
-
D-sorbitol
pH 8.5, 25°C
Gluconobacter oxydans
133.6
-
glycerol
pH 8.5, 25°C
Gluconobacter oxydans
242.8
-
D-ribitol
pH 8.5, 25°C
Gluconobacter oxydans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ba2+
up to 200 mM, 2fold increase in activity
Gluconobacter oxydans
Ca2+
up to 200 mM, 2fold increase in activity
Gluconobacter oxydans
additional information
no effect by addition of Mn2+, Ni2+, Mg2+
Gluconobacter oxydans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28500
-
2 * 28500, SDS-PAGE and calculated, recombinant enzyme; 4 * 28500, SDS-PAGE and calculated, native enzyme
Gluconobacter oxydans
70000
-
gel filtration, recombinant enzyme
Gluconobacter oxydans
140000
-
gel filtration, native enzyme
Gluconobacter oxydans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconobacter oxydans
Q308C1
-
-
Purification (Commentary)
Commentary
Organism
native enzyme
Gluconobacter oxydans
Renatured (Commentary)
Commentary
Organism
inhibition by Cu2+ can partly be restored by EDTA
Gluconobacter oxydans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
12.6
-
substrate D-xylitol, pH 8.5, 25°C
Gluconobacter oxydans
16.4
-
substrate D-xylulose, pH 5.5, 25°C
Gluconobacter oxydans
68.5
-
substrate D-arabitol, pH 8.5, 25°C
Gluconobacter oxydans
Storage Stability
Storage Stability
Organism
4°C, 100 mM Tris/HCl with 2 mM 2-mercaptoethanol, 50 days, 50% oxidative activity
Gluconobacter oxydans
4°C, 100 mM Tris/HCl with 2 mM 2-mercaptoethanol, 80 days, 5% oxidative activity
Gluconobacter oxydans
4°C, 100 mM Tris/HCl without 2 mM 2-mercaptoethanol, 20 days, complete loss of activity
Gluconobacter oxydans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabitol + NAD+
-
701082
Gluconobacter oxydans
D-xylulose + NADH + H+
-
-
-
?
D-arabitol + NADP+
-
701082
Gluconobacter oxydans
D-xylulose + NADPH + H+
-
-
-
r
D-mannitol + NAD+
-
701082
Gluconobacter oxydans
D-fructose + NADH + H+
-
-
-
?
D-ribitol + NAD+
-
701082
Gluconobacter oxydans
D-ribulose + NADH + H+
-
-
-
?
D-sorbitol + NAD+
-
701082
Gluconobacter oxydans
sorbose + NADH + H+
-
-
-
?
D-xylulose + NAD+
-
701082
Gluconobacter oxydans
D-arabitol + NADH
-
-
-
r
glycerol + NAD+
-
701082
Gluconobacter oxydans
dihydroxyacetone + NADH
-
-
-
?
xylitol + NAD+
-
701082
Gluconobacter oxydans
D-xylulose + NADH
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 28500, SDS-PAGE and calculated, recombinant enzyme
Gluconobacter oxydans
tetramer
4 * 28500, SDS-PAGE and calculated, native enzyme
Gluconobacter oxydans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Gluconobacter oxydans
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
50
-
2% of maximum activity
Gluconobacter oxydans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
13.4
-
D-ribitol
pH 8.5, 25°C
Gluconobacter oxydans
16.3
-
glycerol
pH 8.5, 25°C
Gluconobacter oxydans
19.7
-
D-sorbitol
pH 8.5, 25°C
Gluconobacter oxydans
35.3
-
D-mannitol
pH 8.5, 25°C
Gluconobacter oxydans
177.2
-
xylitol
pH 8.5, 25°C
Gluconobacter oxydans
876
-
D-arabitol
pH 8.5, 25°C
Gluconobacter oxydans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
-
ketone reduction
Gluconobacter oxydans
8.5
-
polyol oxidation
Gluconobacter oxydans
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
NADH is preferred over NADPH
Gluconobacter oxydans
NADPH
NADH is preferred over NADPH
Gluconobacter oxydans
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Gluconobacter oxydans
isoelectric focusing
-
5.9
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Gluconobacter oxydans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
NADH is preferred over NADPH
Gluconobacter oxydans
NADPH
NADH is preferred over NADPH
Gluconobacter oxydans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
10 mM, complete inhibition. Up to 80% renaturation by 100 mM EDTA
Gluconobacter oxydans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.5
-
D-arabitol
pH 8.5, 25°C
Gluconobacter oxydans
18.5
-
xylitol
pH 8.5, 25°C
Gluconobacter oxydans
78.5
-
D-mannitol
pH 8.5, 25°C
Gluconobacter oxydans
127.3
-
D-sorbitol
pH 8.5, 25°C
Gluconobacter oxydans
133.6
-
glycerol
pH 8.5, 25°C
Gluconobacter oxydans
242.8
-
D-ribitol
pH 8.5, 25°C
Gluconobacter oxydans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ba2+
up to 200 mM, 2fold increase in activity
Gluconobacter oxydans
Ca2+
up to 200 mM, 2fold increase in activity
Gluconobacter oxydans
additional information
no effect by addition of Mn2+, Ni2+, Mg2+
Gluconobacter oxydans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28500
-
2 * 28500, SDS-PAGE and calculated, recombinant enzyme; 4 * 28500, SDS-PAGE and calculated, native enzyme
Gluconobacter oxydans
70000
-
gel filtration, recombinant enzyme
Gluconobacter oxydans
140000
-
gel filtration, native enzyme
Gluconobacter oxydans
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme
Gluconobacter oxydans
Renatured (Commentary) (protein specific)
Commentary
Organism
inhibition by Cu2+ can partly be restored by EDTA
Gluconobacter oxydans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
12.6
-
substrate D-xylitol, pH 8.5, 25°C
Gluconobacter oxydans
16.4
-
substrate D-xylulose, pH 5.5, 25°C
Gluconobacter oxydans
68.5
-
substrate D-arabitol, pH 8.5, 25°C
Gluconobacter oxydans
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 100 mM Tris/HCl with 2 mM 2-mercaptoethanol, 50 days, 50% oxidative activity
Gluconobacter oxydans
4°C, 100 mM Tris/HCl with 2 mM 2-mercaptoethanol, 80 days, 5% oxidative activity
Gluconobacter oxydans
4°C, 100 mM Tris/HCl without 2 mM 2-mercaptoethanol, 20 days, complete loss of activity
Gluconobacter oxydans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabitol + NAD+
-
701082
Gluconobacter oxydans
D-xylulose + NADH + H+
-
-
-
?
D-arabitol + NADP+
-
701082
Gluconobacter oxydans
D-xylulose + NADPH + H+
-
-
-
r
D-mannitol + NAD+
-
701082
Gluconobacter oxydans
D-fructose + NADH + H+
-
-
-
?
D-ribitol + NAD+
-
701082
Gluconobacter oxydans
D-ribulose + NADH + H+
-
-
-
?
D-sorbitol + NAD+
-
701082
Gluconobacter oxydans
sorbose + NADH + H+
-
-
-
?
D-xylulose + NAD+
-
701082
Gluconobacter oxydans
D-arabitol + NADH
-
-
-
r
glycerol + NAD+
-
701082
Gluconobacter oxydans
dihydroxyacetone + NADH
-
-
-
?
xylitol + NAD+
-
701082
Gluconobacter oxydans
D-xylulose + NADH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 28500, SDS-PAGE and calculated, recombinant enzyme
Gluconobacter oxydans
tetramer
4 * 28500, SDS-PAGE and calculated, native enzyme
Gluconobacter oxydans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Gluconobacter oxydans
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
50
-
2% of maximum activity
Gluconobacter oxydans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
13.4
-
D-ribitol
pH 8.5, 25°C
Gluconobacter oxydans
16.3
-
glycerol
pH 8.5, 25°C
Gluconobacter oxydans
19.7
-
D-sorbitol
pH 8.5, 25°C
Gluconobacter oxydans
35.3
-
D-mannitol
pH 8.5, 25°C
Gluconobacter oxydans
177.2
-
xylitol
pH 8.5, 25°C
Gluconobacter oxydans
876
-
D-arabitol
pH 8.5, 25°C
Gluconobacter oxydans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
-
ketone reduction
Gluconobacter oxydans
8.5
-
polyol oxidation
Gluconobacter oxydans
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Gluconobacter oxydans
isoelectric focusing
-
5.9
Expression
Organism
Commentary
Expression
Gluconobacter oxydans
induced by growth on D-arabinitol
up
Expression (protein specific)
Organism
Commentary
Expression
Gluconobacter oxydans
induced by growth on D-arabinitol
up
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.06
-
D-ribitol
pH 8.5, 25°C
Gluconobacter oxydans
0.12
-
glycerol
pH 8.5, 25°C
Gluconobacter oxydans
0.15
-
D-sorbitol
pH 8.5, 25°C
Gluconobacter oxydans
0.45
-
D-mannitol
pH 8.5, 25°C
Gluconobacter oxydans
9.58
-
xylitol
pH 8.5, 25°C
Gluconobacter oxydans
194.7
-
D-arabitol
pH 8.5, 25°C
Gluconobacter oxydans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.06
-
D-ribitol
pH 8.5, 25°C
Gluconobacter oxydans
0.12
-
glycerol
pH 8.5, 25°C
Gluconobacter oxydans
0.15
-
D-sorbitol
pH 8.5, 25°C
Gluconobacter oxydans
0.45
-
D-mannitol
pH 8.5, 25°C
Gluconobacter oxydans
9.58
-
xylitol
pH 8.5, 25°C
Gluconobacter oxydans
194.7
-
D-arabitol
pH 8.5, 25°C
Gluconobacter oxydans
Other publictions for EC 1.1.1.287
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
701082
Cheng
Cloning, purification and char ...
Gluconobacter oxydans
Protein J.
28
263-272
2009
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-
1
-
-
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1
6
-
3
3
-
-
1
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-
1
-
1
-
3
3
8
2
1
1
-
6
-
2
-
2
-
1
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-
1
2
-
-
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-
1
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6
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3
3
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-
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-
1
1
-
3
3
8
2
1
1
-
6
-
2
-
1
1
-
-
1
6
6
657867
Link
Characterization of a novel NA ...
Uromyces viciae-fabae
Biochem. J.
389
289-295
2005
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1
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11
1
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1
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5
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1
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2
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5
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2
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2
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1
2
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11
1
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1
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1
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2
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5
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2
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658781
Cheng
Molecular cloning and function ...
Gluconobacter oxydans, Gluconobacter oxydans CGMCC 1.110
FEMS Microbiol. Lett.
252
35-42
2005
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-
1
-
-
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-
7
1
-
1
2
-
2
-
-
1
-
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1
7
-
12
1
1
-
-
7
2
-
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1
-
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-
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-
1
1
-
-
-
-
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-
7
1
-
1
2
-
-
-
1
-
1
7
-
12
1
1
-
-
7
2
-
-
-
-
-
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