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show all sequences of 1.1.1.287

Molecular cloning and functional expression of D-arabitol dehydrogenase gene from Gluconobacter oxydans in Escherichia coli

Cheng, H.; Jiang, N.; Shen, A.; Feng, Y.; FEMS Microbiol. Lett. 252, 35-42 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene ArDH, DNA and amino acid sequence determination and analysis, gene ArDH is part of an operon with several components of a sugar transporter system, subcloning in Escherichia coli strain TG-1, functional expression in Escherichia coli strain BL21(DE3) as His6-tagged enzyme
Gluconobacter oxydans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
12.5
-
D-arabitol
pH 8.5, 25°C
Gluconobacter oxydans
18.5
-
D-mannitol
pH 8.5, 25°C
Gluconobacter oxydans
25
-
D-xylulose
pH 6.0, 25°C
Gluconobacter oxydans
47
-
D-fructose
pH 6.0, 25°C
Gluconobacter oxydans
125
-
D-sorbitol
pH 8.5, 25°C
Gluconobacter oxydans
266
-
meso-erythritol
pH 8.5, 25°C
Gluconobacter oxydans
384
-
glycerol
pH 8.5, 25°C
Gluconobacter oxydans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
bound
Gluconobacter oxydans
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
x * 29000, recombinant enzyme, SDS-PAGE
Gluconobacter oxydans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-arabitinol + NADP+
Gluconobacter oxydans
-
D-xylulose + NADPH
-
-
?
D-arabitinol + NADP+
Gluconobacter oxydans CGMCC 1.110
-
D-xylulose + NADPH
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconobacter oxydans
Q58LW6
-
-
Gluconobacter oxydans CGMCC 1.110
Q58LW6
-
-
Purification (Commentary)
Commentary
Organism
recombinant His6-tagged enzyme from Escherichia coli
Gluconobacter oxydans
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:arabitol-grown cell
-
Gluconobacter oxydans
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8
-
purified recombinant enzyme, substrate glycerol
Gluconobacter oxydans
3.2
-
purified recombinant enzyme, substrate meso-erythritol
Gluconobacter oxydans
6.4
-
purified recombinant enzyme, substrate D-sorbitol
Gluconobacter oxydans
7.9
-
purified recombinant enzyme, substrate D-fructose
Gluconobacter oxydans
11.4
-
purified recombinant enzyme, substrate D-xylulose
Gluconobacter oxydans
23.5
-
purified recombinant enzyme, substrate D-mannitol
Gluconobacter oxydans
27.6
-
purified recombinant enzyme, substrate D-arabitol
Gluconobacter oxydans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabitinol + NADP+
-
658781
Gluconobacter oxydans
D-xylulose + NADPH
-
-
-
?
D-arabitinol + NADP+
-
658781
Gluconobacter oxydans CGMCC 1.110
D-xylulose + NADPH
-
-
-
?
D-arabitol + NADP+
preferred substrate for the forward and reverse reaction, respectively
658781
Gluconobacter oxydans
D-xylulose + NADPH + H+
-
-
-
r
D-arabitol + NADP+
preferred substrate for the forward and reverse reaction, respectively
658781
Gluconobacter oxydans CGMCC 1.110
D-xylulose + NADPH + H+
-
-
-
r
D-mannitol + NADP+
preferred substrate for the forward and reverse reaction, respectively
658781
Gluconobacter oxydans
D-fructose + NADPH + H+
-
-
-
r
D-mannitol + NADP+
preferred substrate for the forward and reverse reaction, respectively
658781
Gluconobacter oxydans CGMCC 1.110
D-fructose + NADPH + H+
-
-
-
r
D-sorbitol + NADP+
-
658781
Gluconobacter oxydans
sorbose + NADPH
-
-
-
r
D-sorbitol + NADP+
-
658781
Gluconobacter oxydans CGMCC 1.110
sorbose + NADPH
-
-
-
r
glycerol + NADP+
-
658781
Gluconobacter oxydans
? + NADPH
-
-
-
r
meso-erythritol + NADP+
-
658781
Gluconobacter oxydans
L-erythrose + NADPH
-
-
-
r
additional information
substrate specificity for different sugars, overview, no activity with xylitol, L-arabitol, and L-sorbitol
658781
Gluconobacter oxydans
?
-
-
-
-
additional information
substrate specificity for different sugars, overview, no activity with xylitol, L-arabitol, and L-sorbitol
658781
Gluconobacter oxydans CGMCC 1.110
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 29000, recombinant enzyme, SDS-PAGE
Gluconobacter oxydans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Gluconobacter oxydans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.28
-
meso-erythritol
pH 8.5, 25°C
Gluconobacter oxydans
1.33
-
glycerol
pH 8.5, 25°C
Gluconobacter oxydans
2.57
-
D-sorbitol
pH 8.5, 25°C
Gluconobacter oxydans
196
-
D-xylulose
pH 6.0, 25°C
Gluconobacter oxydans
224
-
D-fructose
pH 6.0, 25°C
Gluconobacter oxydans
243
-
D-mannitol
pH 8.5, 25°C
Gluconobacter oxydans
258
-
D-arabitol
pH 8.5, 25°C
Gluconobacter oxydans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
reverse reaction
Gluconobacter oxydans
8.5
-
forward reaction
Gluconobacter oxydans
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
dependent on
Gluconobacter oxydans
Cloned(Commentary) (protein specific)
Commentary
Organism
gene ArDH, DNA and amino acid sequence determination and analysis, gene ArDH is part of an operon with several components of a sugar transporter system, subcloning in Escherichia coli strain TG-1, functional expression in Escherichia coli strain BL21(DE3) as His6-tagged enzyme
Gluconobacter oxydans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
dependent on
Gluconobacter oxydans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
12.5
-
D-arabitol
pH 8.5, 25°C
Gluconobacter oxydans
18.5
-
D-mannitol
pH 8.5, 25°C
Gluconobacter oxydans
25
-
D-xylulose
pH 6.0, 25°C
Gluconobacter oxydans
47
-
D-fructose
pH 6.0, 25°C
Gluconobacter oxydans
125
-
D-sorbitol
pH 8.5, 25°C
Gluconobacter oxydans
266
-
meso-erythritol
pH 8.5, 25°C
Gluconobacter oxydans
384
-
glycerol
pH 8.5, 25°C
Gluconobacter oxydans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
bound
Gluconobacter oxydans
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
x * 29000, recombinant enzyme, SDS-PAGE
Gluconobacter oxydans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-arabitinol + NADP+
Gluconobacter oxydans
-
D-xylulose + NADPH
-
-
?
D-arabitinol + NADP+
Gluconobacter oxydans CGMCC 1.110
-
D-xylulose + NADPH
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His6-tagged enzyme from Escherichia coli
Gluconobacter oxydans
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:arabitol-grown cell
-
Gluconobacter oxydans
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8
-
purified recombinant enzyme, substrate glycerol
Gluconobacter oxydans
3.2
-
purified recombinant enzyme, substrate meso-erythritol
Gluconobacter oxydans
6.4
-
purified recombinant enzyme, substrate D-sorbitol
Gluconobacter oxydans
7.9
-
purified recombinant enzyme, substrate D-fructose
Gluconobacter oxydans
11.4
-
purified recombinant enzyme, substrate D-xylulose
Gluconobacter oxydans
23.5
-
purified recombinant enzyme, substrate D-mannitol
Gluconobacter oxydans
27.6
-
purified recombinant enzyme, substrate D-arabitol
Gluconobacter oxydans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabitinol + NADP+
-
658781
Gluconobacter oxydans
D-xylulose + NADPH
-
-
-
?
D-arabitinol + NADP+
-
658781
Gluconobacter oxydans CGMCC 1.110
D-xylulose + NADPH
-
-
-
?
D-arabitol + NADP+
preferred substrate for the forward and reverse reaction, respectively
658781
Gluconobacter oxydans
D-xylulose + NADPH + H+
-
-
-
r
D-arabitol + NADP+
preferred substrate for the forward and reverse reaction, respectively
658781
Gluconobacter oxydans CGMCC 1.110
D-xylulose + NADPH + H+
-
-
-
r
D-mannitol + NADP+
preferred substrate for the forward and reverse reaction, respectively
658781
Gluconobacter oxydans
D-fructose + NADPH + H+
-
-
-
r
D-mannitol + NADP+
preferred substrate for the forward and reverse reaction, respectively
658781
Gluconobacter oxydans CGMCC 1.110
D-fructose + NADPH + H+
-
-
-
r
D-sorbitol + NADP+
-
658781
Gluconobacter oxydans
sorbose + NADPH
-
-
-
r
D-sorbitol + NADP+
-
658781
Gluconobacter oxydans CGMCC 1.110
sorbose + NADPH
-
-
-
r
glycerol + NADP+
-
658781
Gluconobacter oxydans
? + NADPH
-
-
-
r
meso-erythritol + NADP+
-
658781
Gluconobacter oxydans
L-erythrose + NADPH
-
-
-
r
additional information
substrate specificity for different sugars, overview, no activity with xylitol, L-arabitol, and L-sorbitol
658781
Gluconobacter oxydans
?
-
-
-
-
additional information
substrate specificity for different sugars, overview, no activity with xylitol, L-arabitol, and L-sorbitol
658781
Gluconobacter oxydans CGMCC 1.110
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 29000, recombinant enzyme, SDS-PAGE
Gluconobacter oxydans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Gluconobacter oxydans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.28
-
meso-erythritol
pH 8.5, 25°C
Gluconobacter oxydans
1.33
-
glycerol
pH 8.5, 25°C
Gluconobacter oxydans
2.57
-
D-sorbitol
pH 8.5, 25°C
Gluconobacter oxydans
196
-
D-xylulose
pH 6.0, 25°C
Gluconobacter oxydans
224
-
D-fructose
pH 6.0, 25°C
Gluconobacter oxydans
243
-
D-mannitol
pH 8.5, 25°C
Gluconobacter oxydans
258
-
D-arabitol
pH 8.5, 25°C
Gluconobacter oxydans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
reverse reaction
Gluconobacter oxydans
8.5
-
forward reaction
Gluconobacter oxydans
Other publictions for EC 1.1.1.287
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
701082
Cheng
Cloning, purification and char ...
Gluconobacter oxydans
Protein J.
28
263-272
2009
-
-
1
-
-
-
1
6
-
3
3
-
-
1
-
-
1
-
1
-
3
3
8
2
1
1
-
6
-
2
-
2
-
1
-
-
-
1
2
-
-
-
-
1
-
6
-
3
3
-
-
-
-
1
1
-
3
3
8
2
1
1
-
6
-
2
-
1
1
-
-
1
6
6
657867
Link
Characterization of a novel NA ...
Uromyces viciae-fabae
Biochem. J.
389
289-295
2005
-
-
1
-
-
-
-
11
1
-
-
1
-
5
-
-
1
-
-
2
-
-
5
-
-
-
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
11
1
-
-
1
-
-
-
1
-
2
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
658781
Cheng
Molecular cloning and function ...
Gluconobacter oxydans, Gluconobacter oxydans CGMCC 1.110
FEMS Microbiol. Lett.
252
35-42
2005
-
-
1
-
-
-
-
7
1
-
1
2
-
2
-
-
1
-
-
1
7
-
12
1
1
-
-
7
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
7
1
-
1
2
-
-
-
1
-
1
7
-
12
1
1
-
-
7
2
-
-
-
-
-
-
-
-
-