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show all sequences of 1.1.1.287

Characterization of a novel NADP(+)-dependent D-arabitol dehydrogenase from the plant pathogen Uromyces fabae

Link, T.; Lohaus, G.; Heiser, I.; Mendgen, K.; Hahn, M.; Voegele, R.T.; Biochem. J. 389, 289-295 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene ARD1, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae strain 23344c, overexpression in Escherichia coli strain BL21(DE3) as N-terminally His6-tagged protein
Uromyces viciae-fabae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Uromyces viciae-fabae
0.07
-
NADP+
pH 9.0, with D-mannitol
Uromyces viciae-fabae
0.08
-
NADP+
pH 9.0, with D-arabitinol
Uromyces viciae-fabae
0.16
-
NADPH
pH 6.0, with D-fructose
Uromyces viciae-fabae
0.2
-
NADPH
pH 6.0, with D-ribulose
Uromyces viciae-fabae
0.26
-
NADPH
pH 6.0, with D-xylulose
Uromyces viciae-fabae
7.8
-
D-xylulose
pH 6.0
Uromyces viciae-fabae
53.9
-
D-ribulose
pH 6.0
Uromyces viciae-fabae
160
-
D-fructose
pH 6.0
Uromyces viciae-fabae
425
-
D-mannitol
pH 9.0
Uromyces viciae-fabae
834
-
D-arabitinol
pH 9.0
Uromyces viciae-fabae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Uromyces viciae-fabae
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Uromyces viciae-fabae
the enzyme activity is highly increased during infection processes and pathogenesis, development after infection, overview
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Uromyces viciae-fabae
Q4R0J7
cultivated on artificial surfaces in in vitro infection structures
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
Uromyces viciae-fabae
Source Tissue
Source Tissue
Commentary
Organism
Textmining
haustorium
exclusively, in the lumen
Uromyces viciae-fabae
-
additional information
cultivated on artificial surfaces in in vitro infection structures
Uromyces viciae-fabae
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabitinol + NADP+
-
657867
Uromyces viciae-fabae
D-xylulose + NADPH
-
-
-
r
D-arabitinol + NADP+
-
657867
Uromyces viciae-fabae
D-ribulose + NADPH
-
-
-
r
D-mannitol + NADP+
-
657867
Uromyces viciae-fabae
D-fructose + NADPH + H+
-
-
-
r
additional information
the enzyme activity is highly increased during infection processes and pathogenesis, development after infection, overview
657867
Uromyces viciae-fabae
?
-
-
-
-
additional information
the enzyme shows high substrate specificity, the reverse reaction is preferred
657867
Uromyces viciae-fabae
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
assay at, reverse reaction
Uromyces viciae-fabae
9
-
assay at, forward reaction
Uromyces viciae-fabae
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
dependent on, absolutely specific for, forward reaction
Uromyces viciae-fabae
NADPH
dependent on, absolutely specific for, reverse reaction
Uromyces viciae-fabae
Cloned(Commentary) (protein specific)
Commentary
Organism
gene ARD1, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae strain 23344c, overexpression in Escherichia coli strain BL21(DE3) as N-terminally His6-tagged protein
Uromyces viciae-fabae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
dependent on, absolutely specific for, forward reaction
Uromyces viciae-fabae
NADPH
dependent on, absolutely specific for, reverse reaction
Uromyces viciae-fabae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Uromyces viciae-fabae
0.07
-
NADP+
pH 9.0, with D-mannitol
Uromyces viciae-fabae
0.08
-
NADP+
pH 9.0, with D-arabitinol
Uromyces viciae-fabae
0.16
-
NADPH
pH 6.0, with D-fructose
Uromyces viciae-fabae
0.2
-
NADPH
pH 6.0, with D-ribulose
Uromyces viciae-fabae
0.26
-
NADPH
pH 6.0, with D-xylulose
Uromyces viciae-fabae
7.8
-
D-xylulose
pH 6.0
Uromyces viciae-fabae
53.9
-
D-ribulose
pH 6.0
Uromyces viciae-fabae
160
-
D-fructose
pH 6.0
Uromyces viciae-fabae
425
-
D-mannitol
pH 9.0
Uromyces viciae-fabae
834
-
D-arabitinol
pH 9.0
Uromyces viciae-fabae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Uromyces viciae-fabae
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Uromyces viciae-fabae
the enzyme activity is highly increased during infection processes and pathogenesis, development after infection, overview
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
Uromyces viciae-fabae
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
haustorium
exclusively, in the lumen
Uromyces viciae-fabae
-
additional information
cultivated on artificial surfaces in in vitro infection structures
Uromyces viciae-fabae
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabitinol + NADP+
-
657867
Uromyces viciae-fabae
D-xylulose + NADPH
-
-
-
r
D-arabitinol + NADP+
-
657867
Uromyces viciae-fabae
D-ribulose + NADPH
-
-
-
r
D-mannitol + NADP+
-
657867
Uromyces viciae-fabae
D-fructose + NADPH + H+
-
-
-
r
additional information
the enzyme activity is highly increased during infection processes and pathogenesis, development after infection, overview
657867
Uromyces viciae-fabae
?
-
-
-
-
additional information
the enzyme shows high substrate specificity, the reverse reaction is preferred
657867
Uromyces viciae-fabae
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
assay at, reverse reaction
Uromyces viciae-fabae
9
-
assay at, forward reaction
Uromyces viciae-fabae
Other publictions for EC 1.1.1.287
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
701082
Cheng
Cloning, purification and char ...
Gluconobacter oxydans
Protein J.
28
263-272
2009
-
-
1
-
-
-
1
6
-
3
3
-
-
1
-
-
1
-
1
-
3
3
8
2
1
1
-
6
-
2
-
2
-
1
-
-
-
1
2
-
-
-
-
1
-
6
-
3
3
-
-
-
-
1
1
-
3
3
8
2
1
1
-
6
-
2
-
1
1
-
-
1
6
6
657867
Link
Characterization of a novel NA ...
Uromyces viciae-fabae
Biochem. J.
389
289-295
2005
-
-
1
-
-
-
-
11
1
-
-
1
-
5
-
-
1
-
-
2
-
-
5
-
-
-
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
11
1
-
-
1
-
-
-
1
-
2
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
658781
Cheng
Molecular cloning and function ...
Gluconobacter oxydans, Gluconobacter oxydans CGMCC 1.110
FEMS Microbiol. Lett.
252
35-42
2005
-
-
1
-
-
-
-
7
1
-
1
2
-
2
-
-
1
-
-
1
7
-
12
1
1
-
-
7
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
7
1
-
1
2
-
-
-
1
-
1
7
-
12
1
1
-
-
7
2
-
-
-
-
-
-
-
-
-