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Literature summary for 1.1.1.284 extracted from

  • Justino, M.C.; Parente, M.R.; Boneca, I.G.; Saraiva, L.M.
    FrxA is an S-nitrosoglutathione reductase enzyme that contributes to Helicobacter pylori pathogenicity (2014), FEBS J., 281, 4495-4505.
    View publication on PubMed
Search for more literature for 1.1.1.284

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0041
-
 S-nitrosoglutathione pH 7.5, 37°C Helicobacter pylori
0.0072
-
 S-nitrosocysteine pH 7.5, 37°C Helicobacter pylori
0.0143
-
 nitrofurazone pH 7.5, 37°C Helicobacter pylori
0.0224
-
 metronidazole pH 7.5, 37°C Helicobacter pylori
0.0423
-
 Nitrofurantoin pH 7.5, 37°C Helicobacter pylori
0.0626
-
 3-nitrotyrosine pH 7.5, 37°C Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 2 * 26000, SDS-PAGE Helicobacter pylori
52000
-
 gel filtration Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-nitrotyrosine + NADPH + H+
-
 Helicobacter pylori ? + NADP+
-
 ?
metronidazole + NADPH + H+
-
 Helicobacter pylori ? + NADP+
-
 ?
nitrofurantoin + NADPH + H+
-
 Helicobacter pylori ? + NADP+
-
 ?
nitrofurazone + NADPH + H+
-
 Helicobacter pylori ? + NADP+
-
 ?
S-nitrosocysteine + NADPH + H+
-
 Helicobacter pylori ? + NADP+
-
 ?
S-nitrosoglutathione + NADPH + H+
-
 Helicobacter pylori ? + NADP+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 26000, SDS-PAGE Helicobacter pylori

Synonyms

Synonyms Comment Organism
FrxA
-
 Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 3-nitrotyrosine pH 7.5, 37°C Helicobacter pylori
0.5
-
 S-nitrosoglutathione pH 7.5, 37°C Helicobacter pylori
0.5
-
 Nitrofurantoin pH 7.5, 37°C Helicobacter pylori
0.6
-
 metronidazole pH 7.5, 37°C Helicobacter pylori
0.6
-
 S-nitrosocysteine pH 7.5, 37°C Helicobacter pylori
1
-
 nitrofurazone pH 7.5, 37°C Helicobacter pylori

Cofactor

Cofactor Comment Organism Structure
FMN 0.2 FMN molecules per FrxA polypeptide chain Helicobacter pylori

Expression

Organism Comment Expression
Helicobacter pylori transcription of FrxA is induced by nitrosative stress up

General Information

General Information Comment Organism
physiological function inactivation of the FrxA-encoding gene results in a strain more sensitive to S-nitrosoglutathione. FrxA contributes to the proliferation of Helicobacter pylori in macrophages and FrxA supports the virulence of the pathogen upon mouse infection Helicobacter pylori

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7
-
 3-nitrotyrosine pH 7.5, 37°C Helicobacter pylori
11
-
 Nitrofurantoin pH 7.5, 37°C Helicobacter pylori
25
-
 metronidazole pH 7.5, 37°C Helicobacter pylori
71
-
 nitrofurazone pH 7.5, 37°C Helicobacter pylori
89
-
 S-nitrosocysteine pH 7.5, 37°C Helicobacter pylori
126
-
 S-nitrosoglutathione pH 7.5, 37°C Helicobacter pylori