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Literature summary for 1.1.1.284 extracted from

  • Huang, C.; Ken, C.; Wen, L.; Lin, C.
    An enzyme possessing both glutathione-dependent formaldehyde dehydrogenase and S-nitrosoglutathione reductase from Antrodia camphorata (2009), Food Chem., 112, 795-802.
    View publication on PubMed
Search for more literature for 1.1.1.284

Cloned(Commentary)

Cloned (Comment) Organism
subcloned into NdeI and NotI sites of pET-20b(+) vector and overexpressed in Escherichia coli BL21(DE)pLysS Taiwanofungus camphoratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 S-nitrosoglutathione pH 8.0, 25°C Taiwanofungus camphoratus
1.2
-
 S-(hydroxymethyl)glutathione pH 8.0, 25°C Taiwanofungus camphoratus

Organism

Organism UniProt Comment Textmining
Taiwanofungus camphoratus D2CFJ2
-
-

Purification (Commentary)

Purification (Comment) Organism
by Ni-NTA affinity chromatography Taiwanofungus camphoratus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-(hydroxymethyl)glutathione + NAD+
-
 Taiwanofungus camphoratus S-formylglutathione + NADH + H+
-
 ?
S-nitrosoglutathione + NADH + H+
-
 Taiwanofungus camphoratus ?
-
 ?

Synonyms

Synonyms Comment Organism
GFD
-
 Taiwanofungus camphoratus
Glutathione-dependent formaldehyde dehydrogenase
-
 Taiwanofungus camphoratus
GSH-FDH
-
 Taiwanofungus camphoratus
GSNOR
-
 Taiwanofungus camphoratus
S-nitrosoglutathione reductase
-
 Taiwanofungus camphoratus

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Taiwanofungus camphoratus

General Information

General Information Comment Organism
physiological function the enzyme GFD is bifunctional. In addition to the glutathione-dependent formaldehyde dehydrogenase activity, it also functions as an effective S-nitrosoglutathione reductase presumably to safeguard against nitrosative stress Taiwanofungus camphoratus