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Literature summary for 1.1.1.284 extracted from
- Formaldehyde dehydrogenase from human liver. Purification, properties, and evidence for the formation of glutathione thiol esters by the enzyme (1974), J. Biol. Chem., 249, 7653-7663.
General Stability
| General Stability | Organism |
|---|---|
| one freezing at -20°C results in full inactivation | Homo sapiens |
| overnight dialysis to remove mercaptoethanol destroys 60% of its original activity | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | NADH protects more effectively than NAD+ | Homo sapiens |  |
| formaldehyde | - |
Homo sapiens | |
| Hg2+ | NADH protects more effectively than NAD+ | Homo sapiens | |
| iodoacetate | NADH protects more effectively than NAD+ | Homo sapiens | |
| methylglyoxal | - |
Homo sapiens | |
| NAD+ | inhibits reverse reaction with NAD+ or NADP+ | Homo sapiens | |
| NADH | inhibits reaction with formaldehyde, glutathione and NAD+ | Homo sapiens | |
| NADPH | inhibits reaction with formaldehyde, glutathione and NAD+ | Homo sapiens | |
| S-formylglutathione | inhibits reaction with formaldehyde, glutathione and NAD+ | Homo sapiens | |
| S-formylglutathione | product inhibition with S-hydroxymethylglutathione | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0042 | - |
NADH | pH 5.7 | Homo sapiens | |
| 0.0081 | - |
glutathione | pH 8.0 | Homo sapiens | |
| 0.009 | - |
NAD+ | pH 8.0 | Homo sapiens | |
| 0.0136 | - |
NAD+ | pH 5.7 | Homo sapiens | |
| 0.016 | - |
NADH | pH 8.0 | Homo sapiens | |
| 0.045 | - |
NADPH | pH 5.7 | Homo sapiens | |
| 0.28 | - |
S-formylglutathione | pH 5.7, with NADPH as cofactor | Homo sapiens | |
| 0.3 | - |
S-formylglutathione | pH 5.7, with NADH as cofactor | Homo sapiens | |
| 0.57 | - |
NADP+ | pH 5.7 | Homo sapiens | |
| 4 | - |
pyruvylglutathione | pH 5.7, with NADH as cofactor | Homo sapiens | |
| 9.5 | - |
NADP+ | pH 8.0 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 81400 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
| Ovis aries | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Bos taurus | - |
| ascites tumor cell | - |
Mus musculus | - |
| brain | - |
Homo sapiens | - |
| brain | - |
Rattus norvegicus | - |
| brain | - |
Bos taurus | - |
| brain | - |
Oryctolagus cuniculus | - |
| cardiac muscle | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Mus musculus | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Bos taurus | - |
| liver | - |
Oryctolagus cuniculus | - |
| liver | - |
Ovis aries | - |
| skeletal muscle | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.2 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, in presence of 2 mM mercaptoethanol, 50% loss of activity | Homo sapiens |
| 0°C, 50% loss of activity after 1 month | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-ethoxy-2-hydroxybutyraldehyde + glutathione + NAD+ | - |
Homo sapiens | S-(3-ethoxy-2-hydroxybutyryl)-glutathione + NADH + H+ | - |
? | |
| formaldehyde + glutathione + NAD+ | - |
Mus musculus | S-formylglutathione + NADH + H+ | - |
? | |
| formaldehyde + glutathione + NAD+ | - |
Homo sapiens | S-formylglutathione + NADH + H+ | - |
r | |
| formaldehyde + glutathione + NAD+ | - |
Rattus norvegicus | S-formylglutathione + NADH + H+ | - |
r | |
| formaldehyde + glutathione + NAD+ | - |
Bos taurus | S-formylglutathione + NADH + H+ | - |
? | |
| formaldehyde + glutathione + NAD+ | - |
Oryctolagus cuniculus | S-formylglutathione + NADH + H+ | - |
? | |
| formaldehyde + glutathione + NAD+ | - |
Ovis aries | S-formylglutathione + NADH + H+ | - |
? | |
| formaldehyde + glutathione + NADP+ | - |
Homo sapiens | S-formylglutathione + NADPH + H+ | - |
r | |
| formaldehyde + glutathione + NADP+ | - |
Rattus norvegicus | S-formylglutathione + NADPH + H+ | - |
r | |
| glyoxal + glutathione + NAD+ | - |
Homo sapiens | S-oxoacetylglutathione + NADH | - |
? | |
| hydroxypyruvaldehyde + glutathione + NAD+ | - |
Homo sapiens | S-hydroxypyruvylglutathione + NADH | - |
? | |
| methylglyoxal + glutathione + NAD+ | - |
Homo sapiens | S-pyruvylglutathione + NADH | - |
? | |
| methylglyoxal + glutathione + NAD+ | - |
Bos taurus | S-pyruvylglutathione + NADH | - |
? | |
| pyruvylglutathione + NADH + H+ | - |
Homo sapiens | methylglyoxal + NAD+ | - |
r | |
| S-formylglutathione + NADH | - |
Homo sapiens | formaldehyde + glutathione + NAD+ | - |
r | |
| S-formylglutathione + NADH | - |
Rattus norvegicus | formaldehyde + glutathione + NAD+ | - |
r | |
| S-formylglutathione + NADPH | - |
Homo sapiens | formaldehyde + glutathione + NADP+ | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
15 min, without NADH, 40% loss of activity | Homo sapiens |
| 64 | - |
15 min, in presence of NADH, 40% loss of activity | Homo sapiens |
| 68 | - |
15 min, in presence of NADH, 80% loss of activity | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | - |
reaction with S-formylglutathione and NADH | Homo sapiens |
| 6 | - |
reaction with NADP+ as cofactor | Homo sapiens |
| 8 | - |
reaction with NAD+ as cofactor | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Mus musculus | |
| NAD+ | - |
Homo sapiens | |
| NAD+ | - |
Rattus norvegicus | |
| NAD+ | - |
Bos taurus | |
| NAD+ | - |
Oryctolagus cuniculus | |
| NAD+ | - |
Ovis aries | |
| NADH | - |
Homo sapiens | |
| NADPH | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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