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Literature summary for 1.1.1.283 extracted from
- N5,N10-methylenetetrahydromethanopterin reductase from Methanocaldococcus jannaschii also serves as a methylglyoxal reductase (2017), FEBS Lett., 591, 2269-2278 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
NADPH | pH 7, temperature not specified in the publication | Methanocaldococcus jannaschii |  |
| 1.6 | - |
methylglyoxal | pH 7, temperature not specified in the publication | Methanocaldococcus jannaschii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.73 | - |
pH 7, temperature not specified in the publication | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methylglyoxal + NADPH + H+ | - |
Methanocaldococcus jannaschii | lactaldehyde + NADP+ | - |
? | |
| additional information | recombinantly overexpressed F420-dependent N5,N10-methylenetetrahydromethanopterin reductase Mer, EC 1.5.98.2, is able to use NADPH and methylglyoxal to produce lactaldehyde. Mer does not catalyze the reduction of methylglyoxal to lactaldehyde in the presence of reduced Fo, the precursor of cofactor F420 | Methanocaldococcus jannaschii | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Mer | cf. EC 1.5.98.2 | Methanocaldococcus jannaschii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0022 | - |
methylglyoxal | pH 7, temperature not specified in the publication | Methanocaldococcus jannaschii | |
| 0.12 | - |
NADPH | pH 7, temperature not specified in the publication | Methanocaldococcus jannaschii | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0014 | - |
methylglyoxal | pH 7, temperature not specified in the publication | Methanocaldococcus jannaschii | |
| 0.12 | - |
NADPH | pH 7, temperature not specified in the publication | Methanocaldococcus jannaschii | |
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