Application | Comment | Organism |
---|---|---|
synthesis | engineeering of Sacharomyces cerevisiae for specific reduction of a variety of carbonyl compounds by altering the levels of fatty acid synthase Fas, aldo-keto reductase Ypr1 and R-acetoxy ketone reductase Gre2. By choosing the appropriate engineered yeast strain, it is possible to prepare many of the alcohols in high stereochemical purities and good chemical yields. For example, in the reductions of some alpha-unsubstituted beta-keto esters, genetically disabling Fas virtually eliminates production of (3R)-alcohols. Overexpressing (3S)-selective reductase Gre2 in the Fas knockout strain gives yeast cells that reduce these ketones to the corresponding (3S)-alcohols in >98% ee in each case | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q12068 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethyl (2R)-2-methyl-3-oxobutanoate + NADPH + H+ | - |
Saccharomyces cerevisiae | ethyl (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+ | 86% yield, 70% (2R,3S)-enantiomer in a strain lacking fatty acid synthase activity and overexpressing Gre2 | ? | |
ethyl 3-oxobutanoate + NADPH + H+ | - |
Saccharomyces cerevisiae | ethyl (3S)-3-hydroxybutanoate + NADP+ | 83% yield, 98% S-enantiomer in a strain lacking fatty acid synthase activity and overexpressing Gre2 | ? | |
ethyl 3-oxohexanoate + NADPH + H+ | - |
Saccharomyces cerevisiae | ethyl (3S)-3-hydroxyhexanoate + NADP+ | 90% yield, 98% S-enantiomer in a strain lacking fatty acid synthase activity and overexpressing Gre2 | ? | |
ethyl 3-oxopentanoate + NADPH + H+ | - |
Saccharomyces cerevisiae | ethyl (3S)-3-hydroxypentanoate + NADP+ | 87% yield, 98% S-enantiomer in a strain lacking fatty acid synthase activity and overexpressing Gre2 | ? | |
methyl 3-oxobutanoate + NADPH + H+ | - |
Saccharomyces cerevisiae | methyl (3S)-3-hydroxybutanoate + NADP+ | 76% yield, 98% S-enantiomer in a strain lacking fatty acid synthase activity and overexpressing Gre2 | ? | |
methyl 3-oxopentanoate + NADPH + H+ | - |
Saccharomyces cerevisiae | methyl (3S)-3-hydroxypentanoate + NADP+ | 85% yield, 98% S-enantiomer in a strain lacking fatty acid synthase activity and overexpressing Gre2 | ? |
Synonyms | Comment | Organism |
---|---|---|
Gre2 | - |
Saccharomyces cerevisiae |