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show all sequences of 1.1.1.283

Molecular cloning and characterization of two YGL039w genes encoding broad specificity NADPH-dependent aldehyde reductases from Kluyveromyces marxianus strain DMB1

Akita, H.; Watanabe, M.; Suzuki, T.; Nakashima, N.; Hoshino, T.; FEMS Microbiol. Lett. 362, fnv116 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli; expression in Escherichia coli
Kluyveromyces marxianus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.028
-
NADPH
pH 6.0, 25°
Kluyveromyces marxianus
0.034
-
NADPH
pH 6.0, 25°
Kluyveromyces marxianus
0.43
-
methyl glyoxal
pH 6.0, 25°
Kluyveromyces marxianus
1.6
-
methyl glyoxal
pH 6.0, 25°
Kluyveromyces marxianus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35800
-
gel filtration
Kluyveromyces marxianus
36900
-
gel filtration
Kluyveromyces marxianus
37000
-
1 * 37000, SDS-PAGE; 1 * 37000, SDS-PAGE
Kluyveromyces marxianus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Kluyveromyces marxianus
A0A0E4AX59
-
-
Kluyveromyces marxianus DMB1
A0A0E4AX59
-
-
Kluyveromyces marxianus
A0A0E4AY21
-
-
Kluyveromyces marxianus DMB1
A0A0E4AY21
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.96
-
substrate methylglyoxal, pH 6.0, 25°C
Kluyveromyces marxianus
1.1
-
substrate p-anisaldehyde, pH 6.0, 25°C
Kluyveromyces marxianus
2.1
-
substrate octanal, pH 6.0, 25°C; substrate valeraldehyde, pH 6.0, 25°C
Kluyveromyces marxianus
3
-
substrate heptanal, pH 6.0, 25°C
Kluyveromyces marxianus
3.2
-
substrate methyl glyoxal, pH 6.0, 25°C
Kluyveromyces marxianus
3.3
-
substrate isovaleraldehyde, pH 6.0, 25°C
Kluyveromyces marxianus
4.2
-
substrate isovaleraldehyde, pH 6.0, 25°C
Kluyveromyces marxianus
4.5
-
substrate p-anisaldehyde, pH 6.0, 25°C
Kluyveromyces marxianus
5.3
-
substrate octanal, pH 6.0, 25°C
Kluyveromyces marxianus
5.9
-
substrate valeraldehyde, pH 6.0, 25°C
Kluyveromyces marxianus
6.9
-
substrate heptanal, pH 6.0, 25°C
Kluyveromyces marxianus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
heptanal + NADPH + H+
-
736122
Kluyveromyces marxianus
heptan-1-ol + NADP+
-
-
-
ir
heptanal + NADPH + H+
-
736122
Kluyveromyces marxianus DMB1
heptan-1-ol + NADP+
-
-
-
ir
isovaleraldehyde + NADPH + H+
-
736122
Kluyveromyces marxianus
isoamyl alcohol + NADP+
-
-
-
ir
isovaleraldehyde + NADPH + H+
-
736122
Kluyveromyces marxianus DMB1
isoamyl alcohol + NADP+
-
-
-
ir
methyl glyoxal + NADPH + H+
-
736122
Kluyveromyces marxianus
? + NADP+
-
-
-
ir
methyl glyoxal + NADPH + H+
-
736122
Kluyveromyces marxianus DMB1
? + NADP+
-
-
-
ir
additional information
enzyme shows strong activities toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine, D-lactate, L-lactate or pyruvate. Enzyme has no NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol
736122
Kluyveromyces marxianus
?
-
-
-
-
additional information
enzyme shows strong activities toward linear aldehydes, such as heptanal, valeraldehyde and octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine, D-lactate, L-lactate or pyruvate. Enzyme has no NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol
736122
Kluyveromyces marxianus
?
-
-
-
-
additional information
enzyme shows strong activities toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine, D-lactate, L-lactate or pyruvate. Enzyme has no NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol
736122
Kluyveromyces marxianus DMB1
?
-
-
-
-
additional information
enzyme shows strong activities toward linear aldehydes, such as heptanal, valeraldehyde and octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine, D-lactate, L-lactate or pyruvate. Enzyme has no NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol
736122
Kluyveromyces marxianus DMB1
?
-
-
-
-
octanal + NADPH + H+
-
736122
Kluyveromyces marxianus
octan-1-ol + NADP+
-
-
-
ir
octanal + NADPH + H+
-
736122
Kluyveromyces marxianus DMB1
octan-1-ol + NADP+
-
-
-
ir
p-anisaldehyde + NADPH + H+
-
736122
Kluyveromyces marxianus
p-anisalcohol + NADP+
-
-
-
ir
pentanal + NADPH + H+
-
736122
Kluyveromyces marxianus
pentan-1-ol + NADP+
-
-
-
ir
valeraldehyde + NADPH + H+
-
736122
Kluyveromyces marxianus
amyl alcohol + NADP+
-
-
-
ir
Subunits
Subunits
Commentary
Organism
monomer
1 * 37000, SDS-PAGE; 1 * 37000, SDS-PAGE
Kluyveromyces marxianus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Kluyveromyces marxianus
50
-
-
Kluyveromyces marxianus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
below 40°C, 30 min, 80% residual activity; below 40°C, 30 min, 80% residual activity
Kluyveromyces marxianus
50
-
30 min, 26% residual activity; 30 min, complete loss of activity
Kluyveromyces marxianus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.5
-
methyl glyoxal
pH 6.0, 25°
Kluyveromyces marxianus
2.67
-
NADPH
pH 6.0, 25°
Kluyveromyces marxianus
3.67
-
NADPH
pH 6.0, 25°
Kluyveromyces marxianus
4
-
methyl glyoxal
pH 6.0, 25°
Kluyveromyces marxianus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
;
Kluyveromyces marxianus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
NADH is inert; no activity with NADH
Kluyveromyces marxianus
NADPH
;
Kluyveromyces marxianus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Kluyveromyces marxianus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
NADH is inert
Kluyveromyces marxianus
additional information
no activity with NADH
Kluyveromyces marxianus
NADPH
-
Kluyveromyces marxianus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.028
-
NADPH
pH 6.0, 25°
Kluyveromyces marxianus
0.034
-
NADPH
pH 6.0, 25°
Kluyveromyces marxianus
0.43
-
methyl glyoxal
pH 6.0, 25°
Kluyveromyces marxianus
1.6
-
methyl glyoxal
pH 6.0, 25°
Kluyveromyces marxianus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35800
-
gel filtration
Kluyveromyces marxianus
36900
-
gel filtration
Kluyveromyces marxianus
37000
-
1 * 37000, SDS-PAGE
Kluyveromyces marxianus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.96
-
substrate methylglyoxal, pH 6.0, 25°C
Kluyveromyces marxianus
1.1
-
substrate p-anisaldehyde, pH 6.0, 25°C
Kluyveromyces marxianus
2.1
-
substrate octanal, pH 6.0, 25°C; substrate valeraldehyde, pH 6.0, 25°C
Kluyveromyces marxianus
3
-
substrate heptanal, pH 6.0, 25°C
Kluyveromyces marxianus
3.2
-
substrate methyl glyoxal, pH 6.0, 25°C
Kluyveromyces marxianus
3.3
-
substrate isovaleraldehyde, pH 6.0, 25°C
Kluyveromyces marxianus
4.2
-
substrate isovaleraldehyde, pH 6.0, 25°C
Kluyveromyces marxianus
4.5
-
substrate p-anisaldehyde, pH 6.0, 25°C
Kluyveromyces marxianus
5.3
-
substrate octanal, pH 6.0, 25°C
Kluyveromyces marxianus
5.9
-
substrate valeraldehyde, pH 6.0, 25°C
Kluyveromyces marxianus
6.9
-
substrate heptanal, pH 6.0, 25°C
Kluyveromyces marxianus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
heptanal + NADPH + H+
-
736122
Kluyveromyces marxianus
heptan-1-ol + NADP+
-
-
-
ir
heptanal + NADPH + H+
-
736122
Kluyveromyces marxianus DMB1
heptan-1-ol + NADP+
-
-
-
ir
isovaleraldehyde + NADPH + H+
-
736122
Kluyveromyces marxianus
isoamyl alcohol + NADP+
-
-
-
ir
isovaleraldehyde + NADPH + H+
-
736122
Kluyveromyces marxianus DMB1
isoamyl alcohol + NADP+
-
-
-
ir
methyl glyoxal + NADPH + H+
-
736122
Kluyveromyces marxianus
? + NADP+
-
-
-
ir
methyl glyoxal + NADPH + H+
-
736122
Kluyveromyces marxianus DMB1
? + NADP+
-
-
-
ir
additional information
enzyme shows strong activities toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine, D-lactate, L-lactate or pyruvate. Enzyme has no NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol
736122
Kluyveromyces marxianus
?
-
-
-
-
additional information
enzyme shows strong activities toward linear aldehydes, such as heptanal, valeraldehyde and octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine, D-lactate, L-lactate or pyruvate. Enzyme has no NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol
736122
Kluyveromyces marxianus
?
-
-
-
-
additional information
enzyme shows strong activities toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine, D-lactate, L-lactate or pyruvate. Enzyme has no NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol
736122
Kluyveromyces marxianus DMB1
?
-
-
-
-
additional information
enzyme shows strong activities toward linear aldehydes, such as heptanal, valeraldehyde and octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine, D-lactate, L-lactate or pyruvate. Enzyme has no NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol
736122
Kluyveromyces marxianus DMB1
?
-
-
-
-
octanal + NADPH + H+
-
736122
Kluyveromyces marxianus
octan-1-ol + NADP+
-
-
-
ir
octanal + NADPH + H+
-
736122
Kluyveromyces marxianus DMB1
octan-1-ol + NADP+
-
-
-
ir
p-anisaldehyde + NADPH + H+
-
736122
Kluyveromyces marxianus
p-anisalcohol + NADP+
-
-
-
ir
pentanal + NADPH + H+
-
736122
Kluyveromyces marxianus
pentan-1-ol + NADP+
-
-
-
ir
valeraldehyde + NADPH + H+
-
736122
Kluyveromyces marxianus
amyl alcohol + NADP+
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 37000, SDS-PAGE
Kluyveromyces marxianus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Kluyveromyces marxianus
50
-
-
Kluyveromyces marxianus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
below 40°C, 30 min, 80% residual activity
Kluyveromyces marxianus
50
-
30 min, complete loss of activity
Kluyveromyces marxianus
50
-
30 min, 26% residual activity
Kluyveromyces marxianus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.5
-
methyl glyoxal
pH 6.0, 25°
Kluyveromyces marxianus
2.67
-
NADPH
pH 6.0, 25°
Kluyveromyces marxianus
3.67
-
NADPH
pH 6.0, 25°
Kluyveromyces marxianus
4
-
methyl glyoxal
pH 6.0, 25°
Kluyveromyces marxianus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Kluyveromyces marxianus
Other publictions for EC 1.1.1.283
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736122
Akita
Molecular cloning and characte ...
Kluyveromyces marxianus, Kluyveromyces marxianus DMB1
FEMS Microbiol. Lett.
362
fnv116
2015
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1
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4
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3
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9
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-
11
-
26
1
2
-
2
4
1
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2
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2
4
-
-
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4
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4
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-
11
-
26
2
2
-
4
4
2
-
-
-
-
-
-
-
-
-
737753
Guo
Structural insights into the c ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1844
1486-1492
2014
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-
1
1
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2
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1
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1
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741962
Guo
Structural insights into the ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1844
1486-1492
2014
-
-
1
1
1
-
-
-
-
-
-
2
-
2
-
-
1
1
-
-
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-
5
1
1
-
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-
1
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2
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-
1
2
1
1
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-
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-
-
-
-
2
-
-
-
1
-
-
-
-
5
1
1
-
-
-
1
-
-
-
-
3
3
-
-
-
710768
Breicha
Crystallization and preliminar ...
Saccharomyces cerevisiae
Acta Crystallogr. Sect. F
66
838-841
2010
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
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-
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-
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1
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1
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-
-
-
-
692329
Greig
A comparative study of methylg ...
Leishmania major, Leishmania major Friedlin, Trypanosoma brucei, Trypanosoma cruzi
FEBS J.
276
376-386
2009
-
-
-
-
-
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8
-
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3
-
4
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3
-
4
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-
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-
-
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-
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-
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-
686820
Hauser
A transcriptome analysis of is ...
Saccharomyces cerevisiae
FEMS Yeast Res.
7
84-92
2007
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-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
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-
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-
1
-
1
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670170
Xu
Methylglyoxal detoxification b ...
Synechococcus sp.
Microbiology
152
2013-2021
2006
-
-
-
-
-
-
-
11
-
-
-
-
-
3
-
-
-
-
-
-
17
-
18
-
-
-
-
11
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
17
-
18
-
-
-
-
11
1
-
1
-
-
-
-
-
-
-
739760
Warringer
Involvement of yeast YOL151W/G ...
Saccharomyces cerevisiae
Yeast
23
389-398
2006
-
-
-
-
-
-
-
-
-
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1
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-
-
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-
-
-
-
-
-
-
-
1
1
-
-
-
657418
Chen
Associating protein activities ...
Saccharomyces cerevisiae
Yeast
20
545-554
2003
-
-
1
-
-
-
-
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1
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6
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1
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6
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Purification and characterizat ...
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1
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1
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1
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3
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1
1
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1
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1
1
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7
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1
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1
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2
1
6
1
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2
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1
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3
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1
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17
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7
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1
3
1
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1
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2
1
6
1
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2
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Murata
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Metabolism of alpha-ketoglutar ...
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207971
Murata
Metabolism of 2-oxoaldehyde in ...
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6
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8
3
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1
1
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1
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1
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1
1
4
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6
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8
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3
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1
1
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1
1
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1
1
4
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207972
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Phenotypic character of the me ...
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