BRENDA - Enzyme Database show
show all sequences of 1.1.1.283

Associating protein activities with their genes: rapid identification of a gene encoding a methylglyoxal reductase in the yeast Saccharomyces cerevisiae

Chen, C.N.; Porubleva, L.; Shearer, G.; Svrakic, M.; Holden, L.G.; Dover, J.L.; Johnston, M.; Chitnis, P.R.; Kohl, D.H.; Yeast 20, 545-554 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
GRE2
Saccharomyces cerevisiae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.35
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methylglyoxal + NADPH + H+
-
657418
Saccharomyces cerevisiae
(R)-lactataldehyde + NADP+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
GRE2
Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.35
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methylglyoxal + NADPH + H+
-
657418
Saccharomyces cerevisiae
(R)-lactataldehyde + NADP+
-
-
-
?
Other publictions for EC 1.1.1.283
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736122
Akita
Molecular cloning and characte ...
Kluyveromyces marxianus, Kluyveromyces marxianus DMB1
FEMS Microbiol. Lett.
362
fnv116
2015
-
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1
-
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-
4
-
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3
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9
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11
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26
1
2
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2
4
1
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2
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2
4
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4
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4
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11
-
26
2
2
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4
4
2
-
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-
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-
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-
737753
Guo
Structural insights into the c ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1844
1486-1492
2014
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1
1
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741962
Guo
Structural insights into the ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1844
1486-1492
2014
-
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1
1
1
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2
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2
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1
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5
1
1
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1
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3
3
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710768
Breicha
Crystallization and preliminar ...
Saccharomyces cerevisiae
Acta Crystallogr. Sect. F
66
838-841
2010
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1
1
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692329
Greig
A comparative study of methylg ...
Leishmania major, Leishmania major Friedlin, Trypanosoma brucei, Trypanosoma cruzi
FEBS J.
276
376-386
2009
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8
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4
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686820
Hauser
A transcriptome analysis of is ...
Saccharomyces cerevisiae
FEMS Yeast Res.
7
84-92
2007
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1
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3
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670170
Xu
Methylglyoxal detoxification b ...
Synechococcus sp.
Microbiology
152
2013-2021
2006
-
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11
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3
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17
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18
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11
1
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1
1
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1
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11
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17
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18
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11
1
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1
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739760
Warringer
Involvement of yeast YOL151W/G ...
Saccharomyces cerevisiae
Yeast
23
389-398
2006
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657418
Chen
Associating protein activities ...
Saccharomyces cerevisiae
Yeast
20
545-554
2003
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1
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738443
Rodriguez
Highly stereoselective reagent ...
Saccharomyces cerevisiae
J. Am. Chem. Soc.
123
1547-1555
2001
-
1
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1
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6
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6
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207977
Inoue
-
Purification and characterizat ...
Cyberlindnera mrakii
J. Ferment. Bioeng.
71
134-136
1991
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3
2
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1
1
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1
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1
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207974
Inoue
Metabolism of 2-oxoaldehyde in ...
Aspergillus niger
Eur. J. Biochem.
171
213-218
1988
3
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17
7
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1
6
1
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2
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207973
Murata
-
Metabolism of alpha-ketoglutar ...
Saccharomyces cerevisiae
J. Ferment. Technol.
64
1-4
1986
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207971
Murata
Metabolism of 2-oxoaldehyde in ...
Saccharomyces cerevisiae
Eur. J. Biochem.
151
631-636
1985
6
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8
3
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1
1
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1
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1
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1
1
4
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6
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8
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3
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1
1
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1
1
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1
1
4
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207972
Murata
Phenotypic character of the me ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
50
1200-1207
1985
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