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Literature summary for 1.1.1.28 extracted from

  • Chen, C.M.; Chen, S.M.; Chien, P.J.; Yu, H.Y.
    Development of an enzymatic assay system of D-lactate using D-lactate dehydrogenase and a UV-LED fluorescent spectrometer (2015), J. Pharm. Biomed. Anal., 116, 150-155.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-lactate + NAD+ Rattus norvegicus
-
pyruvate + NADH + H+
-
r
(R)-lactate + NAD+ Rattus norvegicus Wistar
-
pyruvate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus A0A0G2K1W9
-
-
Rattus norvegicus Wistar A0A0G2K1W9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
blood serum
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-lactate + NAD+
-
Rattus norvegicus pyruvate + NADH + H+
-
r
(R)-lactate + NAD+ development of an assay system of D-lactate with good precision, accuracy, and sensitivity for the determination of D-lactate concentrations in rat serum. D-Lactate dehydrogenase (D-LDH) is utilized to catalyze D-lactate and NAD+ to pyruvate and NADH, analysis of D-lactate by HPLC, respectively. Structure of the enzymatic assay system with a UV-LED fluorescent spectrometer, and method optimization and validation, overview Rattus norvegicus pyruvate + NADH + H+
-
r
(R)-lactate + NAD+
-
Rattus norvegicus Wistar pyruvate + NADH + H+
-
r
(R)-lactate + NAD+ development of an assay system of D-lactate with good precision, accuracy, and sensitivity for the determination of D-lactate concentrations in rat serum. D-Lactate dehydrogenase (D-LDH) is utilized to catalyze D-lactate and NAD+ to pyruvate and NADH, analysis of D-lactate by HPLC, respectively. Structure of the enzymatic assay system with a UV-LED fluorescent spectrometer, and method optimization and validation, overview Rattus norvegicus Wistar pyruvate + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
D-LDH
-
Rattus norvegicus
ldhd
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
cytochrome c
-
Rattus norvegicus
FAD
-
Rattus norvegicus
NAD+
-
Rattus norvegicus