Cloned (Comment) | Organism |
---|---|
gene ldhD, subcloning in Escherichia coli straiin DH5alpha, functional overexpression of phenyllactic acid-overproducing enzyme mutant in Escherichia coli strain BL21 (DE3), optimal induction at 25°C | Lactiplantibacillus pentosus |
Protein Variants | Comment | Organism |
---|---|---|
Y52A | site-directed mutagenesis, enhancement of phenyllactic acid biosynthesis by recognition site replacement of D-lactate dehydrogenase from Lactobacillus pentosus | Lactiplantibacillus pentosus |
Y52L | site-directed mutagenesis, enhancement of phenyllactic acid biosynthesis by recognition site replacement of D-lactate dehydrogenase from Lactobacillus pentosus | Lactiplantibacillus pentosus |
Y52V | site-directed mutagenesis, enhancement of phenyllactic acid biosynthesis by recognition site replacement of D-lactate dehydrogenase from Lactobacillus pentosus. Escherichia coli pET-28a-d-ldh produces 12.2 g phenyllactate/l in 3 h with a molar conversion rate of 61%, while Escherichia coli pET-28a-d-ldhY52V produces 15.6 g phenyllactate/l with a molar conversion rate of 77%. Site-directed mutagenesis of d-ldh markedly improves D-phenyllactate yield and substrate conversion rate. Phenyllactate yield does not show a significant increase when higher glucose amounts are added. Method optimization, overview | Lactiplantibacillus pentosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-3-phenylpyruvate + NADH + H+ | Lactiplantibacillus pentosus | - |
D-3-phenyllactate + NAD+ | i.e. 2-hydroxy-3-phenylpropanoic acid | r | |
pyruvate + NADH + H+ | Lactiplantibacillus pentosus | - |
(R)-lactate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus pentosus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-3-phenylpyruvate + NADH + H+ | - |
Lactiplantibacillus pentosus | D-3-phenyllactate + NAD+ | i.e. 2-hydroxy-3-phenylpropanoic acid | r | |
additional information | D-LDH is an NADH-dependent reductase, substrate reduction is accompanied by the oxidation of NADH to NAD? | Lactiplantibacillus pentosus | ? | - |
? | |
pyruvate + NADH + H+ | - |
Lactiplantibacillus pentosus | (R)-lactate + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
D-LDH | - |
Lactiplantibacillus pentosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Lactiplantibacillus pentosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Lactiplantibacillus pentosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Lactiplantibacillus pentosus | |
NADH | - |
Lactiplantibacillus pentosus |
General Information | Comment | Organism |
---|---|---|
additional information | the side chain of residue Tyr52 locates very near the C3 substituent group of 2-ketoacid substrate and is associated with the recognition of substrate side chain | Lactiplantibacillus pentosus |